Safety of true bone ceramics

doi:10.3969/j.issn.2095-4344.2013.16.004

Abstract

Abstract:

BACKGROUND: True bone ceramic treated with high temperature has continuous microporous structure, good biological compatibility and degradation, which are analogous to nature bone.
OBJECTIVE: To observe the biocompatibility, cellular compatibility and acute toxicity of implanted composite made from periosteal-derived osteoblasts and sintered bovine bone (true bone ceramics).
METHODS: Experiment of cellular compatibility: bovine true bone ceramics were compound cultured with induced passage 3 bone marrow mesenchymal stem cells of Wistar rats. Hemolysis test: the true bone ceramic water extract, normal saline and double-distilled water were added into rabbit blood. Blood coagulation test: the true bone ceramics were added into rabbit blood plasma. Systemic acute toxicity test: true bone ceramic water extract and normal saline were injected into the Kunming mice via the tail vein respectively. Micronucleus test: true bone ceramic water extract, normal saline and cyclophosphamide were given into the mice through intraperitoneal injection respectively. Local irritation reaction: true bone ceramic water extract and normal saline were injected at the two sides of the spine respectively. Pyrogen test: true bone ceramic water extract was injected via the ear vein. The subcutaneous implantation test: the true bone ceramic materials were implanted dorsal subcutaneously.
RESULTS AND CONCLUSION: True bone ceramics had no cell toxicity, but had good cellular and blood compatibility; had no stimulation to muscle and skin; had no toxicity on heart, liver and kidney; had no stimulation to the surrounding tissues after subcutaneous implantation, and part of the materials were degraded and replaced by newly developed fibrous tissue; had no pyrogenic action and had no influence on coagulation; had no inhibition and toxic effect on bone marrow cells.

Key words: biomaterials, tissue-engineered bone materials, true bone ceramic, cellular compatibility, biocompatibility, toxicity, bone marrow mesenchymal stem cells, water extract, tissue-engineered bone, bone, provincial grants-supported paper

CLC Number:

R318

Liu BY, Guo MF, Xing YX, Ma CG. Safety of true bone ceramics[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(16): 2874-2882.

Figures/Tables 9

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