Acellular tendon of mini-pig inbred-lines is used for Achilles tendon repair in a rabbit model

doi:10.3969/j.issn.2095-4344.2013.16.017

Abstract

Abstract:

BACKGROUND: Allogeneic tendon graft is an ideal method to repair tendon defects. But post-transplantation rejections limit the use of allogeneic tendons.
OBJECTIVE: To investigate the effect of acellular tendon of Banna mini-pig inbred-lines on repair of rabbit Achiles tendon, and then to evaluate the feasibility and validity of porcine-derived xenogeneic graft.
METHODS: Forty Japanese white rabbits were enrolled to prepare Achilles tendon defect models of the both hind legs, and then were randomly divided into two groups: acellular porcine tendon group treated with acellular tendon from Banna mini-pig inbred-lines and autologous tendon group treated with rabbit autologous tendon. End-to-end anastomosis was performed with a 3-0 tendon thread using improved HEMI-KESSLER method.
RESULTS AND CONCLUSION: Within 2 weeks post transplantation, there were no significant differences in white blood cell count and C-reactive protein level between the two groups (P > 0.05). Local reactions in the two groups after transplantation were few, the wound healed at stage Ⅰ, and flexor ankle function returned to normal. Histological results exhibited no lymphocytic infiltration, but interconnected collagen fibers at tendon suture site. These findings indicate that acellular tendons from Banna mini-pig inbred-lines can successfully reconstruct defected rabbit Achilles tendon with good histocompatibility and little rejection.

Key words: biomaterials, extracellular matrix materials, acellular, porcine, tendon grafts, allogeneic tendons, autologous tendons, tendon defects, rabbits, other grants-supported paper

CLC Number:

R318

Jin Xian-hui, Zhang Qing-sheng, Yang Jian-bo, Liu Gui-hua, Wei Wei, Gao Chun-guang, Lin Yue-qiu. Acellular tendon of mini-pig inbred-lines is used for Achilles tendon repair in a rabbit model[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(16): 2968-2972.

Figures/Tables 5

References

[1]Sharma P, Maffulli N.Tendon injury and tendinopathy: healing and repair. J Bone Joint Surg Am. 2005;87(1):187-202.

[2]Zhang YL.Zhonghua Shouwaike Zazhi. 2006;22(3):129-130.张友乐.异体肌腱移植的研究方向与现状[J].中华手外科杂志, 2006,22(3):129-130.

[3]Minami A, Usui M, Ishii S,et al.The in vivo effects of various immunoreactive treatments on allogeneic tendon grafts.J Hand Surg Am. 1983;8(6):888-893.

[4]Tsukazaki S, Kikuchi T, Fujikawa K,et al. Comparative study of the covered area of Leeds-Keio (LK) artificial ligament and radio frequency generated glow discharge treated Leeds-Keio (Bio-LK) ligament with synovial cells. J Long Term Eff Med Implants. 2003;13(4):355-362.

[5]Oberpenning F, Meng J, Yoo JJ, et al. De novo reconstitution of a functional mammalian urinary bladder by tissue engineering.Nat Biotechnol. 1999;17(2):149-155.

[6]Atala A, Freeman MR, Vacanti JP,et al. Implantation in vivo and retrieval of artificial structures consisting of rabbit and human urothelium and human bladder muscle.J Urol. 1993;150(2 Pt 2):608-612.

[7]Hu YY.Zhonghua Guke Zazhi. 2000；20（9）:517-518.胡蕴玉. 把握契机加快我国组织工程学的应用研究[J].中华骨科杂志,2000,20(9):517-518.

[8]Yang ZM. Zhonghua Guke Zazhi. 2000;20(9):519-520.杨志明.加快组织工程学临床基础研究的步伐[J].中华骨科杂志, 2000,20(9):519-520.

[9]Kropp BP, Cheng EY.Bioengineering organs using small intestinal submucosa scaffolds: in vivo tissue-engineering technology.J Endourol. 2000;14(1):59-62.

[10]Arnoczky SP, Warren RF, Ashlock MA. Replacement of the anterior cruciate ligament using a patellar tendon allograft. An experimental study. J Bone Joint Surg Am. 1986;68(3): 376-385.

[11]Nurminskaya MV, Birk DE. Differential expression of genes associated with collagen fibril growth in the chicken tendon: identification of structural and regulatory genes by subtractive hybridization. Arch Biochem Biophys. 1998;350(1):1-9.

[12]Chong AK, Riboh J, Smith RL,et al. Flexor tendon tissue engineering: acellularized and reseeded tendon constructs.Plast Reconstr Surg. 2009;123(6):1759-1766.

[13]Park SN, Lee HJ, Lee KH,et al. Biological characterization of EDC-crosslinked collagen-hyaluronic acid matrix in dermal tissue restoration. Biomaterials. 2003;24(9):1631-1641.

[1]	Duan Liyun, Cao Xiaocang. Human placenta mesenchymal stem cells-derived extracellular vesicles regulate collagen deposition in intestinal mucosa of mice with colitis [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(7): 1026-1031.
[2]	Zou Gang, Xu Zhi, Liu Ziming, Li Yuwan, Yang Jibin, Jin Ying, Zhang Jun, Ge Zhen, Liu Yi. Human acellular amniotic membrane scaffold promotes ligament differentiation of human amniotic mesenchymal stem cells modified by Scleraxis in vitro [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(7): 1037-1044.
[3]	Li Li, Ma Li. Immobilization of lactase on magnetic chitosan microspheres and its effect on enzymatic properties [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(4): 576-581.
[4]	Wang Hao, Chen Mingxue, Li Junkang, Luo Xujiang, Peng Liqing, Li Huo, Huang Bo, Tian Guangzhao, Liu Shuyun, Sui Xiang, Huang Jingxiang, Guo Quanyi, Lu Xiaobo. Decellularized porcine skin matrix for tissue-engineered meniscus scaffold [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(22): 3473-3478.
[5]	Chen Lei, Zheng Rui, Jie Yongsheng, Qi Hui, Sun Lei, Shu Xiong. In vitro evaluation of adipose-derived stromal vascular fraction combined with osteochondral integrated scaffold [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(22): 3487-3492.
[6]	Zhou Anqi, Tang Yufei, Wu Bingfeng, Xiang Lin. Designing of periosteum tissue engineering: combination of generality and individuality [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(22): 3551-3557.
[7]	Lang Limin, He Sheng, Jiang Zengyu, Hu Yiyi, Zhang Zhixing, Liang Minqian. Application progress of conductive composite materials in the field of tissue engineering treatment of myocardial infarction [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(22): 3584-3590.
[8]	Xie Jian, Su Jiansheng. Advantages and characteristics of electrospun aligned nanofibers as scaffolds for tissue engineering [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(16): 2575-2581.
[9]	Ji Qi, Yu Zhengwen, Zhang Jian. Problems and trends of technique and clinical application of metallic biomaterials prepared by three-dimensional printing technology [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(16): 2597-2604.
[10]	Qian Nannan, Zhang Qian, Yang Rui, Ao Jun, Zhang Tao. Mesenchymal stem cells in the treatment of spinal cord injury: cell therapy and combination of new drugs and biomaterials [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(13): 2114-2120.
[11]	Liu Tao, Zhang Nini, Huang Guilin . Relationship between extracellular vesicles and radiation-induced tissue injury [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(13): 2121-2126.
[12]	Liu Zhigang, Guo Qinggong, Chen Jingtao. Effect of Capparis spinosa total alkaloid on proliferation and apoptosis of nucleus pulposus cells in an intervertebral disc degeneration rat model [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(11): 1699-1704.
[13]	Luo Yaxin, Bi Haoran, Chen Xiaoxu, Yang Kun. Extracellular matrix and tissue engineering regeneration and repair [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(11): 1785-1790.
[14]	Jia Wei, Zhang Mandong, Chen Weiyi, Wang Chenyan, Guo Yuan. Effects of femoral prosthetic materials on artificial knee arthroplasty performance [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(10): 1477-1481.
[15]	Li Junqi, Tian Guangzhao, Chen Mingxue, Wang Hao, Liu Shuyun, Sui Xiang, Huang Jingxiang, Li Ming, Guo Quanyi. Regulatory effect of acellular cartilage extracellular matrix on phenotype of mouse macrophage line [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(10): 1512-1516.