Layer-by-layer self-assembly multifunctional heparin surface modification

doi:10.3969/j.issn.2095-4344.2013.16.018

Abstract

Abstract:

BACKGROUND: Surface coating can improve material surface properties and hemocompatibility without changing the original material, and thus reduce complications resulting from implantable devices.
OBJECTIVE: To observe the hemocompatibility and anticoagulation properties of layer-by-layer self-assembly multifunctional heparin coating.
METHODS: Layer-by-layer self-assembly was used to form the self-assembled multilayer coating with two function layers on the surface and the bottom. Surface coating was heparin/Fe ³⁺ multi-layer self-assembly coating and the bottom coating was chitosan, both of which were fastened to the surface of titanium using a covalent bond. There were five layers of heparin. The heparin/chitosan layer-by-layer self-assembly coating with the same number of layers was considered as the multi-layer control group, and the single layer coating of heparin and chitosan blending solution was considered as the single-layer control group; the bare titanium pieces were regarded as the blank control group.
RESULTS AND CONCLUSION: Examination of hemocompatibility showed that the hemolysis rate of the self-assembled multilayer coating was lower than that of the multi-layer control group; toluidine blue staining showed that the total amount of heparin and the heparin dissolved and released amount at 4, 24 and 48 hours of the self-assembled multilayer coating were significantly higher than those of multi-layer control group and single- layer control group; dynamic clotting experimental results showed the self-assembled multilayer coating had the best anticoagulation properties. The results indicate that layer-by-layer self-assembly multifunctional heparin coating has the excellent hemocompatibility and coagulation properties.

Key words: biomaterials, material mechanics and surface modification, layer-by-layer self-assembly, heparin, chitosan, coating, hemocompatibility, anticoagulation property, graft, adverse reaction

CLC Number:

:R318

Wei Wei, Li Guo-rong. Layer-by-layer self-assembly multifunctional heparin surface modification[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(16): 2973-2980.

Figures/Tables 6

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