Reconstructing the perfused-decellularized full-thickness small intestine extracellular matrix

doi:10.3969/j.issn.2095-4344.2013.16.014

Abstract

Abstract:

BACKGROUND: Immersing method is difficult to construct the ideal acellular matrix for the complex organ.
OBJECTIVE: To investigate the effect of preparing the acellular full-thickness small intestine scaffold by
perfusion method.
METHODS: All the small intestines were excised in a sterile fashion in adult male New Zealand rabbits and
the acellular small intestine scaffolds were obtained by perfusing ionic detergents through the superior
mesenteric arteries. Relative growth rates were detected using 3-(4,5-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide method after bone marrow mesenchymal stem cells from 1-month-old NewZealand rabbits were co-cultured with decellularized full-thickness small intestine extracellular matrix
(experimental group) or Dulbecco’s modified Eagle’s medium containing 20% fetal bovine serum (control group).
RESULTS AND CONCLUSION: The macroscopic view showed that the perfused-decellularized small intestine
artery became translucent after 30 minutes of perfusion, and the small intestine segment became translucent
after 2 hours of perfusion. The vessels were seen distinctly. Histological view and scanning electron microscope
results indicated that the perfused-decellularized full-thickness small intestine showed perfect acellular effect.
Many pores and collagen fibers were retained and the porosity was (86.72±2.98)%. 3-(4,5-Dimethylthiazol-2-yl)-
2,5-diphenyltetrazolium bromide assay showed that the relative growth rate of the experimental group overtopped
1. The absorbance values in the experimental group were significantly higher than those in the control group at 2,
4 and 7 days of coculture (P < 0.05). These findings suggest that the perfusion method is a better way to
construct acellular full-thickness small intestine scaffold because it can achieve better acellular effect and retain
scaffold vitality at the greatest extent in the acellular small intestine scaffold that can promote the growth of bone
marrow mesenchymal stem cells and not add toxicity to the cells.

Key words: biomaterials, extracellular matrix materials, perfusion method, decellularized technique, small
intestine, mucous membrane, extracellular matrix, laryngotracheal, bone marrow mesenchymal stem cells;
relative growth rate, provincial grants-supported paper

CLC Number:

R318

Hou Nan, Zhu Li, Wen Ke. Reconstructing the perfused-decellularized full-thickness small intestine extracellular matrix[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(16): 2950-2955.

Figures/Tables 3

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