Silk-poly(lactic-co-glycolic acid) copolymer composite scaffold: Mechanical properties and cytocompatibility

doi:10.3969/j.issn.2095-4344.2013.16.013

Abstract

Abstract:

BACKGROUND: Compared with the usual synthetic fibers, silk has better mechanical properties with a certain degree of ductility, which is a good scaffold material for construction of tissue engineered ligament/tendon. The degradation speed of silk fibroin fibers, however, is too low to correspond to tissue regeneration rate.
OBJECTIVE: To evaluate the mechanical property of a rope-shaped scaffold braided by silk-poly(lactic-co-glycolic acid) and its cytocompatibility with rabbit bone marrow mesenchymal stem cells.
METHODS: Firstly, the hybrid rope-shaped silk-poly(lactic-co-glycolic acid) scaffold was prepared by twisting weave using a mixture of silkworm silk fibroin fiber and PLGA filament, and then modified by fibronectin. Secondly, rabbit bone marrow mesenchymal stem cells were isolated and proliferated in vitro. Then, they were seeded on the surface of the scaffold. The situation of cell growth, matrix formation, as well as the combination of cells and scaffold was observed under an inverted phase contrast microscope and scanning electron microscopy.
RESULTS AND CONCLUSION: The appearance of the scaffold braided by silk-poly(lactic-co-glycolic acid) was milky white, spiral rope-like, and homogeneous with a strong toughness. The diameter of the scaffold was 2.3 mm. The maximum load, tensile strength, breakpoints elongation rates, and elastic modulus of the scaffold were (315.06±30.77) N, (75.83±7.46) MPa, (61.39±7.26)%, (213.58±23.45) MPa, respectively. Scanning electron microscopy observation revealed that rabbit bone marrow mesenchymal stem cells grew and adhered well on the scaffold surface. Most of the bone marrow mesenchymal stem cells showed spindle-shaped with good stretch, and they grew in three dimensions. Moreover, the bone marrow mesenchymal stem cells secreted rich cell matrix on the surface of the scaffold braided by silk-poly(lactic-co-glycolic acid). In short, the scaffold braided by silk-poly(lactic-co-glycolic acid) has good biomechanical properties and cytocompatibility.

Key words: biomaterials, material biocompatibility, silk, poly(lactic-co-glycolic acid) copolymer, rope-shaped scaffold, mechanical property, bone marrow mesenchymal stem cells, cytocompatibility, National Natural Science Foundation of China

CLC Number:

R318

Zhang Wen-yuan, Yang Ya-dong, Li Ying, Zhang Ke-ji, Fang Guo-jian, Tang Liang, Li Yue-zhong, . Silk-poly(lactic-co-glycolic acid) copolymer composite scaffold: Mechanical properties and cytocompatibility[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(16): 2943-2949.

Figures/Tables 3

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