Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (16): 2506-2513.doi: 10.3969/j.issn.2095-4344.3151
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Wang Liang1, Guo Yuxing1, Wu Xun1, Huang Hua2, Yuan Guangyin2, Zhang Lei1
Received:
2020-03-06
Revised:
2020-03-11
Accepted:
2020-05-16
Online:
2021-06-08
Published:
2021-01-07
Contact:
Zhang Lei, Chief physician, Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Peking University, Beijing 100081, China
About author:
Wang Liang, Master candidate, Physician, Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Peking University, Beijing 100081, China
Supported by:
CLC Number:
Wang Liang, Guo Yuxing, Wu Xun, Huang Hua, Yuan Guangyin, Zhang Lei. Antibacterial properties of Jiao Da Bio-Magnesium scaffolds in vitro[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(16): 2506-2513.
2.4 电导率检测结果 细菌在生长过程中与外界环境不断的进行物质交换,包含K+、Na+等小分子物质及蛋白质、链状核苷酸等大分子物质,这些带电物质的跨膜运动使细胞外液体环境的带电粒子数目发生变化,带电粒子总数的变化可用导电率测得。而细胞膜的完整性是有序调控这些物质交换的前提,即细胞外液体环境的导电率可在一定程度上反映细胞膜的完整性。 无论是大肠杆菌还是金黄色葡萄球菌,实验组与对照组细胞外液环境导电率均随时间延长而增大,并且不同时间点对照组导电率均低于实验组(P < 0.05,P < 0.01),见图6。结果表明,实验组细菌细胞内外环境的带电粒子分布出现变化,即材料浸提液对两种细菌细胞膜的完整性有影响。"
2.8 细胞内可溶性蛋白含量检测结果 蛋白质是细胞生命的物质基础,细菌内蛋白质含量的变化可间接反映细菌生命活动的活跃程度。 通过对比蛋白质Marker的染色条带分析可知大肠杆菌的可溶性蛋白质分子质量在10-170 kD之间;而金黄色葡萄球菌的可溶性蛋白质分子质量在10-300 kD之间,分布宽度大于大肠杆菌,见图12。大肠杆菌中实验组与对照组不同时间点各分子质量蛋白质分布无明显差异,表明材料浸提液对大肠杆菌菌体内蛋白质的合成无明显抑制作用;金黄色葡萄球菌中实验组相较于对照组最大分子质量的蛋白质条带变浅,且随时间延长这种差异减弱,表明材料浸提液对金黄色葡萄球菌菌体内蛋白质合成具有抑制作用,进一步推测该结果可能是材料浸提液对金黄色葡萄球菌内维持高分子质量蛋白质结构的某些化学键有破坏作用,或者直接抑制了高分子量蛋白质的合成,而且这种抑制作用随接触时间的延长逐渐变弱。 "
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