Biological properties of nano-hydroxyapatite-zinc oxide composite scaffolds and their effects on the behavior of MC3T3-E1 osteoblasts

doi:10.12307/2023.803

Abstract

Abstract: BACKGROUND: It has been found that the mechanical properties of nano-hydroxyapatite can be improved by doping zinc ions in nano-hydroxyapatite.
OBJECTIVE: To investigate the biological properties of nano-hydroxyapatite ceramic scaffold mixed with different concentrations of zinc oxide, and to study the effects of the scaffold on the biological behavior of MC3T3-E1 osteoblasts.
METHODS: Nano-hydroxyapatite ceramic scaffolds containing different mass fractions (0%, 0.5%, 1.5%, 2.5%, 3.5%) of zinc oxide were prepared by a chitin gel system to characterize the physical properties of scaffolds. The five groups of nano-hydroxyapatite-zinc oxide composite scaffolds were co-cultured with MC3T3-E1 osteoblasts. Calcein AM/PI staining, CCK-8 assay, CellTiter LumiTM Luminescent assay, alkaline phosphatase activity detection, alizarin red staining, and quantitative analysis of calcium ions were detected.
RESULTS AND CONCLUSION: (1) Under a scanning electron microscope, the surface of the nano-hydroxyapatite-zinc oxide composite scaffolds had three-dimensional perforated pores. The pore size of the composite scaffolds was uniform, ranging from 300 to 400 μm, and the porosity was above 70%. With the increase of the mass fraction of zinc oxide, the compressive strength of the composite scaffold increased gradually. (2) Calcein-AM/PI staining showed no obvious cytotoxicity in the five groups, and the survival rate of cells in the 2.5% group was higher than that in the 0% group (P < 0.05). CCK-8 assay and CellTiter-LumiTM luminescent assay showed that cell proliferation and activity of each group were in the order from strong to weak: 2.5% group >1.5% group > 0.5% group > 0% group > 3.5% group. The order of alkaline phosphatase activity from high to low was 2.5% group > 1.5% group > 3.5% group > 0.5% group > 0% group. Alizarin red staining and quantitative analysis of calcium ions showed that the formation ability of cell mineralized nodules was in the order of 2.5% group > 1.5% group > 0.5% group > 3.5% group > 0% group from strong to weak. (3) The results showed that zinc oxide doping improved the mechanical properties of nano-hydroxyapatite scaffolds and promoted the proliferation and differentiation of osteoblasts, among which the composite scaffolds doped with 2.5% zinc oxide had the best proliferation and differentiation ability of osteoblasts.

Key words: hydroxyapatite, zinc oxide, bone tissue engineering, scaffold, bone regeneration, chitin gel

CLC Number:

Liu Zixuan, Li Yan, Ji Lin, Xia Delin. Biological properties of nano-hydroxyapatite-zinc oxide composite scaffolds and their effects on the behavior of MC3T3-E1 osteoblasts[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(34): 5441-5447.

Figures/Tables 12

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