Construction of tissue-engineered bone composite scaffolds by loading rabbit-derived bone marrow mesenchymal stem cells on magnesium-based alloy scaffolds

doi:10.12307/2023.726

Abstract

Abstract: BACKGROUND: With the in-depth research and rapid development of tissue engineering, tissue-engineered scaffolds provide a new opportunity for bone tissue repair.
OBJECTIVE: To explore the cell proliferation of bone marrow mesenchymal stem cells after co-culture with the magnesium-based alloy scaffolds, to provide a new idea for the construction of tissue-engineered bone scaffolds and provide a basis for the clinical application of tissue-engineered scaffolds.
METHODS: The bone marrow fluid of rabbits was extracted under sterile conditions. Bone marrow mesenchymal stem cells were cultured and isolated by the whole bone marrow adherent method. Bone marrow mesenchymal stem cells were identified by flow cytometry and osteogenic differentiation ability was tested. Bone marrow mesenchymal stem cells at passage 3 were seeded on magnesium-based alloy scaffolds and chitosan polycaprolactone tricalcium phosphate scaffolds respectively. The cell growth was observed by laser confocal scanning on days 3 and 10, and cell proliferation was detected using an MTT assay on days 1, 4, 7, 10 and 14, respectively.
RESULTS AND CONCLUSION: (1) The cultured cells highly expressed CD44 and CD90, but hardly CD34. (2) The morphology gradually changed to polygonal after osteogenesis induction. Von Kossa staining showed that the calcium nodules between the cells were stained black, and the alkaline phosphatase activity was significantly higher than that of the control group (P < 0.05). (3) After co-culture of magnesium-based alloy and bone marrow mesenchymal stem cells, confocal scanning showed that the cells could adhere to the scaffold and grow in clusters. With the extension of time, the magnesium-based alloy scaffold was more active in proliferation than the seed cells loaded on the chitosan polycaprolactone tricalcium phosphate scaffold, and the number of cells was significantly increased. MTT assay showed that when the two materials were co-cultured with bone marrow mesenchymal stem cells for 10 days, the cell growth reached its peak. (4) These results indicate that rabbit-derived bone marrow mesenchymal stem cells have a good osteogenic potential. After co-cultured with magnesium-based alloy scaffolds, the cells adhered and grew well on the surface of the scaffolds, indicating that the tissue-engineered magnesium-based implants were successfully constructed.

Key words: bone marrow mesenchymal stem cell, magnesium-based alloy, tissue-engineered bone, cell proliferation, scaffold, coculture

CLC Number:

Zhou Yuebin, Guo Honggang. Construction of tissue-engineered bone composite scaffolds by loading rabbit-derived bone marrow mesenchymal stem cells on magnesium-based alloy scaffolds[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(34): 5491-5496.

Figures/Tables 9

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