Chinese Journal of Tissue Engineering Research ›› 2023, Vol. 27 ›› Issue (6): 840-845.doi: 10.12307/2023.243
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Liu Wentao1, Feng Xingchao2, Yang Yi2, Bai Shengbin1
Received:
2022-02-08
Accepted:
2022-04-18
Online:
2023-02-28
Published:
2022-08-09
Contact:
Bai Shengbin, MD, Professor, School of Basic Medicine, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
Yang Yi, Master, Associate chief physician, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
About author:
Liu Wentao, Master candidate, School of Basic Medicine, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
Supported by:
CLC Number:
Liu Wentao, Feng Xingchao, Yang Yi, Bai Shengbin. Effect of M2 macrophage-derived exosomes on osteogenic differentiation of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(6): 840-845.
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2.5 M2型巨噬细胞来源外泌体促进骨髓间充质干细胞成骨 骨髓间充质干细胞与30,60,90 mg/L M2型巨噬细胞来源外泌体共培养72 h后,其中60 mg/L M2型巨噬细胞来源外泌体组成骨分化相关蛋白RUNX2最高(P < 0.05),与M2型巨噬细胞来源外泌体摄取效率结果一致,见图6A。而且发现骨髓间充质干细胞与60 mg/L M2型巨噬细胞来源外泌体共培养24,48,72 h,成骨分化相关蛋白RUNX2、碱性磷酸酶表达随时间变化逐渐上调(P < 0.05),见图6B。茜素红染色结果显示,随着共培养时间增加,矿物质沉积染色逐渐增强,表明骨髓间充质干细胞向成骨分化,见图7。此外,60 mg/L M2型巨噬细胞来源外泌体组矿物质沉积染色显著大于其余组(P < 0.05),与M2型巨噬细胞来源外泌体摄取效率及蛋白质印迹结果一致,见图8。这些结果均表明M2型巨噬细胞来源外泌体可以促进骨髓间充质干细胞成骨分化。"
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