Chinese Journal of Tissue Engineering Research ›› 2023, Vol. 27 ›› Issue (34): 5483-5490.doi: 10.12307/2023.725
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Wang Ziqi1, 2, Li Xiaoyin2, Jiang Jipeng3, Song Zhen1, 2, Li Zhengchao2, Chen Shulian2, Chen Xuyi2
Received:
2022-10-13
Accepted:
2022-11-18
Online:
2023-12-08
Published:
2023-04-22
Contact:
Chen Xuyi, MD, Researcher, Doctoral supervisor, Research Institute of Maritime Rights Defense Medical Support, Chinese People’s Armed Police Force Characteristic Medical Center, Tianjin Key Laboratory of Neurotrauma Repair, Tianjin 300162, China
About author:
Wang Ziqi, Master candidate, Graduate School of Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; Research Institute of Maritime Rights Defense Medical Support, Chinese People’s Armed Police Force Characteristic Medical Center, Tianjin Key Laboratory of Neurotrauma Repair, Tianjin 300162, China
Li Xiaoyin, Master, Research intern, Research Institute of Maritime Rights Defense Medical Support, Chinese People’s Armed Police Force Characteristic Medical Center, Tianjin Key Laboratory of Neurotrauma Repair, Tianjin 300162, China
Supported by:
CLC Number:
Wang Ziqi, Li Xiaoyin, Jiang Jipeng, Song Zhen, Li Zhengchao, Chen Shulian, Chen Xuyi. Collagen/silk fibroin scaffold combined with human umbilical cord-derived mesenchymal stem cells in the treatment of traumatic brain injury in dogs[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(34): 5483-5490.
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2.4 改良格拉斯哥评分 术后1 d以及1,4,8,12,16,20,24周分别进行改良格拉斯哥评分。术后1 d组间各项改良格拉斯哥评分无明显差异,术后1-16周,干细胞组、支架组改良格拉斯哥评分有高于创伤组的趋势,但无明显统计学意义。术后1,4,8,12,16,20,24周联合组改良格拉斯哥评分极其显著高于创伤组(P < 0.01);术后8,12,16周,联合组改良格拉斯哥评分显著高于干细胞组与支架组(P < 0.05);术后1,4,20,24周,联合组改良格拉斯哥评分极其显著高于干细胞组(P < 0.01);术后1,20,24周,联合组改良格拉斯哥评分极其显著高于支架组(P < 0.01),见图5。"
2.5 运动诱发电位检测结果 2.5.1 振幅比较 干细胞组与支架组在一定时间节点振幅显著高于创伤组,而大部分时间节点显著低于联合组。90 V、1个月和3个月,110 V、6个月时,创伤组振幅均显著或极其显著低于干细胞组(P < 0.05或P < 0.01);除90 V、6个月,其余情况下创伤组振幅均显著或极其显著低于支架组(P < 0.05或P < 0.01);所有检测条件下干细胞组振幅均显著或极其显著低于联合组(P < 0.05或P < 0.01);90 V,1,3,6个月和110 V,6个月情况下,支架组振幅非常显著低于联合组(P < 0.01),见图6。结果表明hUC-MSCs联合CS能够显著提高创伤性脑损伤犬的运动诱发电位振幅,且效果明显优于单纯hUC-MSCs和CS。"
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