Exosomes from human umbilical cord-derived mesenchymal stem cells suppress the proliferation and promote the apoptosis of medulloblastoma Daoy cells

doi:10.12307/2021.134

Abstract

Abstract: BACKGROUND: Mesenchymal stem cells can produce a large number of exosomes, which has similar biological activity to mesenchymal stem cells. However, the effect of mesenchymal stem cell-derived exosomes on Daoy cells of medulloblastoma has yet to be determined.
OBJECTIVE: To investigate the effect of exosomes derived from human umbilical cord-derived mesenchymal stem cells on the proliferation and apoptosis of medulloblastoma Daoy cells and its potential mechanism.
METHODS: Human umbilical cord-derived mesenchymal stem cells were isolated and cultured. Passage 4 of human umbilical cord-derived mesenchymal stem cells was collected. The expression of exosome markers CD9 and CD81 was detected by western blot assay. Exosomes extracted from human umbilical cord-derived mesenchymal stem cells were cocultured with Daoy cells for 96 hours. The proliferation of Daoy cells was detected by flow cytometry and colony formation assay. The apoptosis of Daoy cells was detected by flow cytometry. The expression levels of apoptosis related proteins were detected by western blot assay.
RESULTS AND CONCLUSION: Western blot assay results showed that exosomes from human umbilical cord-derived mesenchymal stem cells were positive for CD9 and CD81. Compared with the control group, exosomes from human umbilical cord-derived mesenchymal stem cells inhibited the proliferation of Daoy cells and the formation of colonies (P < 0.05, P < 0.01), and promoted Daoy cell apoptosis (P < 0.01). Apoptosis related proteins Cyclin D1 and Bcl-2 were down regulated (P < 0.05), but expression level of Caspase-3 was up-regulated (P < 0.01). The results confirm that human umbilical cord-derived mesenchymal stem cells derived exosomes can suppress the proliferation of Daoy cells and promote its apoptosis possibly by regulating cell cycle and apoptosis related protein expression.

Key words: stem cells, human umbilical cord-derived mesenchymal stem cells, exosomes, medulloblastoma, Daoy cells, apoptosis, protein

CLC Number:

Yi Zhishuang, Wang Zhihai, Li Ming, Zhou Qingan, Yang Baosheng. Exosomes from human umbilical cord-derived mesenchymal stem cells suppress the proliferation and promote the apoptosis of medulloblastoma Daoy cells[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(31): 4945-4949.

Figures/Tables 4

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