Effects of Gushukang on transforming growth factor beta1 and beta-catenin during molar intrusion in Beagle dogs

doi:10.12307/2023.511

Abstract

Abstract: BACKGROUND: Gushukang can effectively promote apoptosis of osteoclasts, increase bone mineral density and bone mineral content, and inhibit bone resorption. However, studies on root resorption are scarce and its regulatory mechanism remains unclear.
OBJECTIVE: To investigate the effect of Gushukang on root resorption and expression of transforming growth factor-β1 and β-catenin in Beagle dogs.
METHODS: Sixteen Beagle dogs were randomly divided into normal control group (n=4), distilled water group (n=6), and Gushukang group (n=6). Normal control group did not do any treatment. Distilled water group and Gushukang group established Maxillary first molar intrusion models were established in the distilled water and Gushukang groups. After modeling, distilled water and Gushukang groups were intragastrically given distilled water and Gushukang at a dose of 2.1 g/kg body mass respectively. After 6, 9, and 12 weeks of intervention, Beagle dogs were anesthetized to collect root and periodontal tissues of the maxillary first molars. The pathological changes were observed by hematoxylin-eosin staining and the expression levels of transforming growth factor-β1 and β-catenin were detected by immunohistochemistry.
RESULTS AND CONCLUSION: The maxillary first molars were obviously intruded over time, especially at the 12th week. The results of hematoxylin-eosin staining revealed that the number of osteoclasts in molar tissue increased in the distilled water group with the prolongation of loading, accompanied by aggravated bone resorption. The Gushukang group had less resorption of molar root and better ability of bone repair and periodontal tissue remodeling than the distilled water group. The expression levels of transforming growth factor-β1 and β-catenin in the distilled water group were significantly lower than those in the normal control group (P < 0.05), while the expression levels of transforming growth factor-β1 and β-catenin in molar tissue of the Gushukang group increased with time, which were significantly higher than those in the distilled water group at the 9th and 12th weeks (P < 0.05). To conclude, Gushukang could regulate transforming growth factor-β1 and β-catenin and promote osteogenic differentiation of molar bone tissue in Beagle dogs.

Key words: root absorption, Gushukang, transforming growth factor-β1, , β-catenin, Beagle dog, orthodontic tooth

CLC Number:

Wan Zhe, Du Jun, Hu Yang. Effects of Gushukang on transforming growth factor beta1 and beta-catenin during molar intrusion in Beagle dogs[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(28): 4502-4506.

Figures/Tables 3

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