Chinese Journal of Tissue Engineering Research ›› 2023, Vol. 27 ›› Issue (5): 683-689.doi: 10.12307/2023.086
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Wu Yujie1, 2, Wan Xiaofang1, 2, Wei Mianxing1, 2, Peng Shiyuan1, 2, Xu Xiaomei1, 2
Received:
2022-02-28
Accepted:
2022-04-24
Online:
2023-02-18
Published:
2022-07-22
Contact:
Xu Xiaomei, MD, Chief physician, Associate professor, Department of Orthodontics, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China; Oral & Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, Sichuan Province, China
About author:
Wu Yujie, Master candidate, Department of Orthodontics, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China; Oral & Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, Sichuan Province, China
Supported by:
CLC Number:
Wu Yujie, Wan Xiaofang, Wei Mianxing, Peng Shiyuan, Xu Xiaomei. Correlation between autophagy and the Hippo-YAP protein pathway in periodental ligament cells on the pressure side of a mouse model of orthodontic tooth movement[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(5): 683-689.
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加力30 min组压力侧牙周膜内可见active-YAP的平均吸光度值增加(P < 0.01);加力1 h其平均吸光度值最强(P < 0.01);加力1-4 h组逐渐减少,至4 h组active-YAP的平均吸光度值达最弱,但仍强于空白组(P < 0.01);加力12 h组再次增强(P < 0.01),随后逐渐减弱,至加力7 d组与空白组无差异(P > 0.05),见图9及表1。 2.6 Beclin-1、LC3B与active-YAP表达变化的相关性分析 实验结果中压力侧牙周膜中Beclin-1和LC3B的表达变化趋势与active-YAP趋势相似,表达强度都在加力1 h达峰值。随后逐渐降低,于加力4 h降至谷值,加力12 h它们的表达增强达又一峰值,而后回落,于7 d都降至基础水平,见图10。 "
[1] XU J, ZHAO X, ZENG J, et al. Role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats. J Dent Sci. 2020;15(3):351-363. [2] COBBAUT M, KARAGIL S, BRUNO L, et al. Dysfunctional Mechanotransduction through the YAP/TAZ/Hippo Pathway as a Feature of Chronic Disease. Cells. 2020;9(1):151. [3] GAO Y, LUO C, RUI T, et al. Autophagy inhibition facilitates wound closure partially dependent on the YAP/IL-33 signaling in a mouse model of skin wound healing. FASEB J. 2021;35(10):e21920. [4] MAYR A, MARCINIAK J, EGGERS B, et al. Autophagy Induces Expression of IL-6 in Human Periodontal Ligament Fibroblasts Under Mechanical Load and Overload and Effects Osteoclastogenesis in vitro. Front Physiol. 2021;12:716441. [5] RAVANAN P, SRIKUMAR IF, TALWAR P. Autophagy: the spotlight for cellular stress responses. Life Sci. 2017;188:53-67. [6] GALATI S, BONI C, GERRA MC, et al. Autophagy: A Player in response to Oxidative Stress and DNA Damage. Oxid Med Cell Longev. 2019; 2019:5692958. [7] CHEN L, MO S, HUA Y. Compressive force-induced autophagy in periodontal ligament cells downregulates osteoclastogenesis during tooth movement. J Periodontol. 2019;90(10):1170-1181. [8] LI W, ZHAO J, SUN W, et al. Osteocytes promote osteoclastogenesis via autophagy-mediated RANKL secretion under mechanical compressive force. Arch Biochem Biophys. 2020;694:108594. [9] KAZAMA T. Electron microscopic study of periodontal tissues in the pressure side by intermittent forces. Tsurumi Shigaku. 1989;15:87-108. [10] LI Y, JACOX LA, COATS S, et al. Roles of autophagy in orthodontic tooth movement. Am J Orthod Dentofacial Orthop. 2021;159(5):582-593. [11] 尹圆圆,马华钰,李昕怡,等.小鼠正畸牙移动中牙周组织自噬相关基因表达的初步研究[J].国际口腔医学杂志,2020,47(6):627-634. [12] MAEJIMA Y, ZABLOCKI D, NAH J, et al. The role of the Hippo pathway in autophagy in the heart [published online ahead of print, 2022 Feb 12]. Cardiovasc Res. 2022; cvac014. [13] ZHOU J, HU M, HE M, et al. TNFAIP3 Interacting Protein 3 Is an Activator of Hippo-YAP Signaling Protecting Against Hepatic Ischemia/Reperfusion Injury. Hepatology. 2021;74(4):2133-2153. [14] TANG F, GAO R, JEEVAN-RAJ B, et al. LATS1 but not LATS2 represses autophagy by a kinase-independent scaffold function. Nat Commun. 2019;10(1):5755. [15] DUPONT S. Role of YAP/TAZ in cell-matrix adhesion-mediated signalling and mechanotransduction. Exp Cell Res. 2016;343(1):42-53. [16] PAVEL M, PARK SJ, FRAKE RA, et al. α-Catenin levels determine direction of YAP/TAZ response to autophagy perturbation. Nat Commun. 2021; 12(1):1703. [17] LIU Z, ZENG W, WANG S, et al. A potential role for the Hippo pathway protein, YAP, in controlling proliferation, cell cycle progression, and autophagy in BCPAP and KI thyroid papillary carcinoma cells. Am J Transl Res. 2017;9(7):3212-3223. [18] KILKENNY C, BROWNE W, CUTHILL IC, et al. Animal research: reporting in vivo experiments: the ARRIVE guidelines. Br J Pharmacol. 2010; 160(7):1577-1579. [19] NAKAMURA T, YAMASHITA M, IKEGAMI K, et al. Autophagy facilitates type I collagen synthesis in periodontal ligament cells. Sci Rep. 2021;11(1):1291. [20] WESTOVER L, FAULKNER G, FLORES-MIR C, et al. Non-invasive evaluation of periodontal ligament stiffness during orthodontic tooth movement. Angle Orthod. 2019;89:228e34. [21] JIANG N, HE D, MA Y, et al. Force-Induced Autophagy in Periodontal Ligament Stem Cells Modulates M1 Macrophage Polarization via AKT Signaling. Front Cell Dev Biol. 2021;9:666631. [22] HUANG Y, LIU H, GUO R, et al. Long Non-coding RNA FER1L4 Mediates the Autophagy of Periodontal Ligament Stem Cells Under Orthodontic Compressive Force via AKT/FOXO3 Pathway. Front Cell Dev Biol. 2021; 9:631181. [23] DAS J, AGARWAL T, CHAKRABORTY S, et al. Compressive stress-induced autophagy promotes invasion of HeLa cells by facilitating protein turnover in vitro. Exp Cell Res. 2019;381(2):201-207. [24] 吕佳岭,徐洁,曾锦,等.正畸牙压力区牙周膜细胞自噬相关蛋白Beclin-1与微管相关蛋白2轻链3的表达[J].华西口腔医学杂志, 2019,37(2):168-173. [25] HUANG L, YIN X, CHEN J, et al. Lithium chloride promotes osteogenesis and suppresses apoptosis during orthodontic tooth movement in osteoporotic model via regulating autophagy. Bioact Mater. 2021; 6(10):3074-3084. [26] JABER FA, KHAN NM, ANSARI MY, et al. Autophagy plays an essential role in bone homeostasis. J Cell Physiol. 2019;234(8):12105-12115. [27] TONG X, ZHANG C, WANG D, et al. Suppression of AMP-activated protein kinase reverses osteoprotegerin-induced inhibition of osteoclast differentiation by reducing autophagy. Cell Prolif. 2020;53(1):e12714. [28] SHI J, WANG L, ZHANG H, et al. Glucocorticoids: Dose-related effects on osteoclast formation and function via reactive oxygen species and autophagy. Bone. 2015;79:222-232. [29] LI J, SUN Z, LIN Y, et al. Syndecan 4 contributes to osteoclast differentiation induced by RANKL through enhancing autophagy. Int Immunopharmacol. 2021;91:107275. [30] TOTARO A, ZHUANG Q, PANCIERA T, et al. Cell phenotypic plasticity requires autophagic flux driven by YAP/TAZ mechanotransduction. Proc Natl Acad Sci USA. 2019;116(36):17848-17857. [31] SHEN Y, PAN Y, GUO S, et al. The roles of mechanosensitive ion channels and associated downstream MAPK signaling pathways in PDLC mechanotransduction. Mol Med Rep. 2020;21(5):2113-2122. [32] JIANG Y, GUAN Y, LAN Y, et al. Mechanosensitive Piezo1 in Periodontal Ligament Cells Promotes Alveolar Bone Remodeling During Orthodontic Tooth Movement. Front Physiol. 2021;12:767136. [33] BENHAM-PYLE BW, PRUITT BL, NELSON WJ. Cell adhesion. Mechanical strain induces E-cadherin-dependent Yap1 and β-catenin activation to drive cell cycle entry. Science. 2015;348(6238):1024-1027. [34] MENG Z, QIU Y, LIN KC, et al. RAP2 mediates mechanoresponses of the Hippo pathway. Nature. 2018;560(7720):655-660. [35] DUPONT S, MORSUT L, ARAGONA M, et al. Role of YAP/TAZ in mechanotransduction. Nature. 2011;474(7350):179-183. [36] NGUYEN MT, LEE W. MiR-141-3p regulates myogenic differentiation in C2C12 myoblasts via CFL2-YAP-mediated mechanotransduction. BMB Rep. 2022;55(2):104-109. [37] PANCIERA T, AZZOLIN L, CORDENONSI M, et al. Mechanobiology of YAP and TAZ in physiology and disease. Nat Rev Mol Cell Biol. 2017;18:758-770. [38] SUN B, WEN Y, WU X, et al. Expression pattern of YAP and TAZ during orthodontic tooth movement in rats. J Mol Histol. 2018;49(2):123-131. [39] WANG D, HE J, HUANG B, et al. Emerging role of the Hippo pathway in autophagy. Cell Death Dis. 2020;11(10):880. [40] PAVEL M, RENNA M, PARK SJ, et al. Contact inhibition controls cell survival and proliferation via YAP/TAZ-autophagy axis. Nat Commun. 2018;9(1):2961. [41] WANG C, ZHU X, FENG W, et al. Verteporfin inhibits YAP function through up-regulating 14-3-3δ sequestering YAP in the cytoplasm. Am J Cancer Res. 2015;6(1):27-37. |
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