Microtubule-associated protein 1 light chain 3 is involved in the occurrence and development of steroid-induced avascular necrosis of the femoral head in a rabbit model

doi:10.12307/2022.930

Abstract

Abstract: BACKGROUND: Steroid-induced avascular necrosis of the femoral head is still a difficult problem, and it is of great significance to study its pathogenesis.
OBJECTIVE: To discuss the role of autophagy gene microtubule-associated protein 1 light chain 3 (MAP1LC3) in steroid-induced avascular necrosis of the femoral head and the effect of 3-methyladenine on MAP1LC3.
METHODS: A total of 36 healthy New Zealand white rabbits, aged 5 months, were randomly divided into 3 groups, with 12 rabbits in each group. Control group was given intramuscular injection of normal saline, 2 mL per rabbit, once a week, four times in total. Model group was given intramuscular injection of 4 mg/kg methylprednisolone, once a week, four times in total. 3-Methyladenine group was given intramuscular injection of 4 mg/kg methylprednisolone followed by intramuscular injection of 3-methyladenine, 2 μL per rabbit, once a week, four times in total. Three animals from each group were sacrificed at 1, 2, 3, and 4 weeks for sampling. The femoral heads of the bilateral hindlimbs were taken. Empty bone lacunae were counted under optical microscope. mRNA and protein expression levels of MAP1LC3 were detected by RT-PCR and western blot assay, respectively.
RESULTS AND CONCLUSION: Compared with the control group, the rate of empty lacunae increased significantly in the model and 3-methyladenine groups (P < 0.05), but the rate of empty lacunae was significantly lower in the 3-methyladenine group than the model group (P < 0.05). The expression of MAP1LC3 mRNA in the model group was higher than that in the 3-methyladenine group (P < 0.05), indicating the activation of autophagy is inhibited by 3-methyladenine. These findings indicate that MAP1LC3 is expressed in the animal model, confirming that autophagy plays a certain role in the pathogenesis of steroid-induced avascular necrosis of the femoral head; 3-methyladenine inhibits the expression of MAP1LC3 and reduces the occurrence and development of steroid-induced avascular necrosis of the femoral head.

Key words: steroid-induced avascular necrosis of the femoral head, autophagy, MAP1LC3, 3-methyladenine, animal model

CLC Number:

Ma Hailong, Zhao Zhenqun, Liu Wanlin, Sun Jun. Microtubule-associated protein 1 light chain 3 is involved in the occurrence and development of steroid-induced avascular necrosis of the femoral head in a rabbit model[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(32): 5167-5172.

Figures/Tables 8

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