中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (15): 2772-2775.doi: 10.3969/j.issn.1673-8225.2012.15.024

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

卵巢切除联合过度运动制作骨性关节炎大鼠模型*☆

苏  云1，孟祥俊2   

  1. 大连大学附属中山医院，1骨科，   2眼科，辽宁省大连市  116001
  • 收稿日期:2011-09-24 修回日期:2011-10-26 出版日期:2012-04-08 发布日期:2012-04-08
  • 作者简介:苏云☆，男，1977 年生，吉林省公主岭市人，汉族，2007 年吉林大学毕业，博士，副主任医师，主要从事骨关节创伤、骨病和骨科人工材料方面的研究。suyunll@sina.com
  • 基金资助:

    大连大学博士启动基金资助项目：卵巢切除联合过度运动制作大鼠骨性关节炎模型的实验研究

A rat osteoarthritis model established by ovariectomy and excessive exercise

Su Yun1, Meng Xiang-jun2   

  1. 1Department of Orthopedics, 2Department of Ophthalmology, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China
  • Received:2011-09-24 Revised:2011-10-26 Online:2012-04-08 Published:2012-04-08
  • About author:Su Yun☆, Doctor, Associate chief physician, Department of Orthopedics, Affiliated Zhongshan Hospital, Dalian University, Dalian 116001, Liaoning Province, China suyunll@sina.com
  • Supported by:

     the Staring Foundation of Dalian University for Doctors

PDF

398

被引次数

5

摘要：

背景：骨性关节炎多见于绝经期妇女，因此成功地建立一个动物绝经期骨性关节炎模型将为研究此类疾病提供帮助。
目的：建立大鼠绝经期骨性关节炎动物模型。
方法：取4月龄雌性SD大鼠，随机分为正常对照组与实验组。实验组经背部切口切除双侧卵巢，切口愈合后，每天驱使大鼠在滚轮上运动，10 min/次，2次/d；正常对照组大鼠不作任何处理。
结果与结论：正常对照组大鼠关节软骨中细胞层次清楚，细胞形态正常。实验组关节软骨变薄、退变，软骨面粗糙，表层有缺失，细胞数量减少，软骨细胞层次紊乱，骨小粱稀疏，排列紊乱。实验组大鼠股骨骨密度较正常对照组大鼠明显下降       (P < 0.05)。说明卵巢切除联合过度运动可成功建立大鼠绝经期骨性关节炎动物模型。
 

关键词: 骨性关节炎, 绝经期, 动物模型, 卵巢, 大鼠

Abstract:

BACKGROUND: Osteoarthritis is commonly seen in postmenopausal women. Therefore, establishing an osteoarthritis model in postmenopausal animals will contribute to the research of osteoarthritis.
OBJECTIVE: To establish a postmenopausal rat model of osteoarthritis．
METHODS: Female SD rats, aged 4 months, were randomly divided into normal control group and experimental group. The backs of experimental rats were cut open and the both ovaries were removed. The experimental rats were forced to walk in a rolling wheel for 10 minutes twice daily after the incision healed. Rats in normal control group received no treatment.
RESULTS AND CONCLUSION: The cell layers in the articular cartilage were clear in the normal control group, and the cell shape was normal. The articular cartilage in the experimental group became thinning and degeneration. The cartilage surface became roughness and some of the surface layer lost. The cell number decreased and the cartilage cell layers became disorder. The trabecula was sparse and arranged in disorder. The femur bone mineral density in experimental group was significantly decreased compared with that in the control group (P < 0.05). It demonstrates that the osteoarthritis model is successfully established by ovariectomy and excessive exercise in postmenopausal rats.

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