中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (15): 2776-2779.doi: 10.3969/j.issn.1673-8225.2012.15.025

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

动脉粥样化模型大鼠主动脉血管细胞黏附分子1 mRNA表达与补阳还五汤的干预*★

张红珍1，陈燕清2，耿艳军3   

  1. 山西中医学院，  1中西医结合系， 2基础系，山西省太原市  \030024；3山西省中西医结合医院，山西省太原市  030009
  • 收稿日期:2011-10-20 修回日期:2011-11-28 出版日期:2012-04-08 发布日期:2012-04-08
  • 通讯作者: 陈燕清，硕士，副教授，山西中医学院基础系，山西省太原市 030024 evidencebased@126.com 并列通讯作者：耿艳军，副主任营养师，山西省中西医结合医院，山西省太原市 030009 gyj4630195@yahoo.com.cn
  • 作者简介:张红珍★，女，1968年生，2005年山西医科大学大学毕业，硕士，副教授，主要从事中西医结合内科教学与临床工作。 zhangjieer@sina.com
  • 基金资助:

    山西省卫生厅科技攻关项目(200958)，课题名称：补阳还五汤对动脉粥样硬化模型主动脉血管细胞黏附分子1mRNA表达量的影响。

Influence of Bu Yang Huan Wu Tang on the expression of aortic vascular cell adhesion molecule 1 mRNA in a rat atherosclerosis model 

Zhang Hong-zhen1, Chen Yan-qing2, Geng Yan-jun3   

  1. 1Department of Integrative Medicine, 2Basis Department, Shanxi College of Traditional Chinese Medicine, Taiyuan  030024, Shanxi Province, China; 3Hospital of Integrated Chinese and Western Medicine of Shanxi Province, Taiyuan  030009, Shanxi Province, China
  • Received:2011-10-20 Revised:2011-11-28 Online:2012-04-08 Published:2012-04-08
  • Contact: author: Chen Yan-qing, Master, Associate chief physician, Basis Department, Shanxi College of Traditional Chinese Medicine, Taiyuan 030024, Shanxi Province, China evidencebased@126.com Corresponding author: Geng Yan-jun, Associate chief nutritionist, Hospital of Integrated Chinese and Western Medicine of Shanxi Province, Taiyuan 030009, Shanxi Province, China gyj4630195@yahoo.com.cn
  • About author:Zhang Hong-zhen★, Master, Associate professor, Department of Integrative Medicine, Shanxi College of Traditional Chinese Medicine, Taiyuan 030024, Shanxi Province, China zhangjieer@sina.com
  • Supported by:

    Scientific and Technological Project of Shanxi Health Department, No.200958*

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摘要：

背景：血管细胞黏附分子1基因表达产物在动脉粥样硬化发生过程中扮演重要角色；补阳还五汤具有改变血液流变学的性质、降低血脂、抗动脉硬化的功效。
目的：观察补阳还五汤对动脉粥样硬化模型的治疗作用，并在分子水平探讨其作用机制。
方法：高脂饮食加维生素D3诱导大鼠动脉粥样硬化模型。32只造模成功的大鼠随机摸球法均分为4组干预：补阳还五汤组、川芎嗪组、模型组和正常对照组。观察各组血脂水平及主动脉中血管细胞黏附分子1 mRNA的表达。
结果与结论：造模成功率为92%。各组血脂水平比较：补阳还五汤组指标接近正常，与其他两组比较，差异有显著性意义。各组血管细胞黏附分子1 mRNA表达比较：正常对照组无表达，补阳还五汤组显著低于模型组和川芎嗪组。说明益气活血代表方剂补阳还五汤可以下调主动脉组织血管细胞黏附分子1 mRNA的表达，降低血脂，具有抗动脉粥样硬化作用，其机制可能与降低主动脉组织血管细胞黏附分子1 mRNA的表达有关。
关键词：补阳还五汤；动脉粥样硬化；血管细胞黏附分子；川芎嗪；大鼠模型
doi:10.3969/j.issn.1673-8225.2012.15.025

关键词: 补阳还五汤, 动脉粥样硬化, 血管细胞黏附分子, 川芎嗪, 大鼠模型

Abstract:

BACKGROUND: Gene expression product of vascular cellular adhesion molecule-1 (VCAM-1) mRNA plays an important role in atherosclerosis. Bu Yang Huan Wu Tang has the effect of changing the quality of blood rheology, decreasing the level of blood-lipid and against atherosclerosis.
OBJECTIVE: To observe the therapeutic effect of Bu Yang Huan Wu Tang on the treatment of atherosclerosis model, meanwhile, to investigate the mechanism on molecule level.
METHODS: High fat diet and Vitamin D3 were used to induce a rat atherosclerosis model. After the AS rats model establishement, thirty-two rats model were divided into four groups according to random touch ball method: Bu Yang Huan Wu Tang therapy group, ligustrazine therapy group, model group and normal control group. The level of blood lipid and the expression of VCAM-1 mRNA in each group were observed.
RESULTS AND CONCLUSION: The rate of molding success was 92%. The level of blood lipid in Bu Yang Huan Wu Tang therapy group was close to normal level, and there was no significant difference when compare with other two groups. There was no expression of VCAM-1 mRNA in normal control group, and compared with ligustrazine therapy group and model group, the expression of VCAM-1 mRNA in Bu Yang Huan Wu Tang therapy group was decreased significantly. It indicates that representative formula of qi-benifiting and blood-activating Chinese medicinal herbs Bu Yang Huan Wu Tang can down-regulate the expression of VCAM-1 mRNA, and decrease the level of blood-lipid in rat models. It has the effect against atherosclerosis, and the mechanism may be related with the down-regulated expression of VCAM-1mRNA.
Zhang HZ, Chen YQ, Geng YJ. Influence of Bu Yang Huan Wu Tang on the expression of aortic vascular cell adhesion molecule 1 mRNA in rat atherosclerosis model. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(15): 2776-2779.    
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