中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (15): 2674-2680.doi: 10.3969/j.issn.1673-8225.2012.15.004

• 骨组织构建 bone tissue construction • 上一篇    下一篇

带蒂筋膜瓣促非细胞型组织工程化骨血管化及其成骨的组织学变化****★

王耀一1，杨新明2，胡振顺1，孟宪勇2，张  瑛2，阴彦林2，张军威2   

  1. 1河北北方学院，河北省张家口市   075000; 2河北北方学院附属第一医院骨科，河北省张家口市   075000
  • 收稿日期:2011-10-22 修回日期:2011-12-02 出版日期:2012-04-08 发布日期:2012-04-08
  • 通讯作者: 杨新明，主任医师，教授，硕士生导师，河北北方学院附属第一医院骨科，河北省张家口市 075000 yxm1120@sohu.com
  • 作者简介:王耀一★，男，1983年生，河北省邯郸市人，汉族，河北北方学院在读硕士，主要从事创伤脊柱、组织工程研究。
  • 基金资助:

    河北省卫生厅《河北省2010年医学科学研究重点课题计划》(20100481)；河北省科技厅2009年度河北省科学技术研究与发展计划，医学科学研究重点课题(09276102D-46)；河北省张家口市2008年科学技术与发展指令计划 (0807045D-10)；河北北方学院2009年校级科研指令计划(2009036)。

Histological changes of vascularization and osteogenesis in the non-cell based tissue engineered bone promoted by the pedicle fascia flap

Wang Yao-yi 1, Yang Xin-ming2, Hu Zhen-shun1, Meng Xian-yong2, Zhang Ying2, Yin Yan-lin2, Zhang Jun-wei2   

  1. 1Hebei North University, Zhangjiakou  075000, Hebei Province, China; 2Department of Orthopedics, the First Affiliated Hospital of Hebei Northern University, Zhangjiakou  075000, Hebei Province, China
  • Received:2011-10-22 Revised:2011-12-02 Online:2012-04-08 Published:2012-04-08
  • Contact: author: Yang Xin-ming, Chief physician, Professor, Master’s supervisor, Department of Orthopedics, the First Affiliated Hospital of Hebei Northern University, Zhangjiakou 075000, Hebei Province, China yxm1120@sohu.com
  • About author:Wang Yao-yi★, Studying for master’s degree, Hebei North University, Zhangjiakou 075000, Hebei Province, China
  • Supported by:

    Hebei Provincial Key Medical Research Project in 2010 of Hebei Health Department, 20100481*; Hebei Provincial Science and Technology Development Project in 2009 of Science and Technology Department of Hebei Province, Key Medical Research Program, No. 09276102D-46*; Science and Technology Development Mandatory Plan of Zhangjiakou in 2008, No. 0807045D-10*; Research Mandatory Plan of Hebei North University, No. 2009036* 

PDF

316

摘要：

背景：目前关于带蒂筋膜瓣促组织工程骨成骨是否以早期促血管化为核心的成骨修复方式的对比研究还缺少相关报道。
目的：通过带蒂筋膜瓣在修复骨缺损各时间段中促非细胞型组织工程化骨血管化及其对成骨作用影响组织学观察，论证带蒂筋膜瓣具有较好的促血管化作用及促成骨能力。
方法：制作兔尺骨长段骨-骨膜完全缺损模型及带蒂筋膜瓣，随机分为单纯植入组、无蒂膜瓣包裹组、血管内皮生长因子组、膜瓣包裹组4组，分别于骨缺损处植入相应材料。
结果与结论：组织学显示，在各时间点无论新生血管数量，还是新生骨小梁质与量，膜瓣包裹组均明显优其他3组。血管再生面积和新生骨小梁面积测定显示，第4周血管内皮生长因子组、膜瓣包裹组明显多于单纯植入组、无蒂膜瓣包裹组        (P < 0.05)；植入后第8，12，16周膜瓣包裹组骨修复区血管再生面积及对应的再生骨小梁面积明显多于其他3组(P < 0.05)。提示带蒂筋膜瓣具有显著促非细胞型组织工程化骨血管化作用，血管化增强有利于成骨作用，且可有效提高成骨质和量、缩短骨修复时间。
 

关键词: 带蒂筋膜瓣, 组织工程骨, 促血管化, 血管内皮生长因子, 骨缺损, 组织学

Abstract:

BACKGROUND: The pedicle fascia flap promotes the osteogenesis of tissue engineered bone. However, it is rarely reported that weather the promotion effect is achieved through the osteogenic repair process with vascularization-centric in the early stage.
OBJECTIVE: To demonstrate that pedicle fascial flap has a good vascularization effect and can promote osteogenesis by observing the effects of pedicle fascial flap on the vascularization and osteogenesis at different stages of bone defect repair in non-cell based tissue engineered bone.
METHODS: Full-defect rabbit models of ulna long segment bone-periosteum and pedicle fascial flap were constructed and divided into four groups: implantation group, fascia flap without pedicle group, vascular endothelial growth factor group and pedicle fascia flap group. The corresponding materials were implanted into the bone defects..
RESULTS AND CONCLUSION: According to histological observation, the pedicle fascia flap group was superior to the implantation group, fascia flap without pedicle group and vascular endothelial growth factor group not only in the quantity of the new vessels but also in the quantity and quality of the new bone trabeculae at each time point. Vascular regeneration area and new bone trabeculae area of the vascular endothelial growth factor group and pedicle fascia flap group was significantly larger than those of the implantation group and fascia flap without pedicle group in the 4th week after implantation (P < 0.05); the vascular regeneration area and the area of the corresponding new bone trabeculaes in the pedicle fascia flap group was significantly larger than those of the other three groups (P < 0.05). These findings illustrate that pedicle fascia flap has a significant promote effect on the vascularization of the non-cell based tissue engineered bone; the enhanced vascularization is helpful to the osteogenesis, it can effectively improve the quantity and quality of the regenerated bone and shorten the bone repair time.

中图分类号: