中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (53): 9957-9960.doi: 10.3969/j.issn.1673-8225.2011.53.019

• 肾移植 kidney transplantation • 上一篇    下一篇

肾移植后外周血CD4+及CD8+T细胞亚群计数的临床意义

熊海云1，张  雷2，王立明2，赵闻雨2，袁  清2，陈  瑜2，周  琳3，张玲珍3，朱有华2   

  1. 1解放军第九四医院泌尿外科，江西省南昌市  330002；解放军第二军医大学长征医院，2器官移植科，3实验诊断科，上海市  200003
  • 收稿日期:2011-06-25 修回日期:2011-08-25 出版日期:2011-12-31 发布日期:2011-12-31
  • 通讯作者: 张雷，博士，主治医师，解放军第二军医大学长征医院器官移植科，上海市 200003 zl3514@hotmail.com
  • 作者简介:熊海云☆，男，1977年生，江西省南昌市人，汉族，2009年解放军第二军医大学毕业，博士，主治医师，主要从事泌尿外科肾移植基础和临床研究。 jetxiongjet@ hotmail.com

Clinical significance of CD4+ and CD8+ T cell subset counts in renal allograft recipients

Xiong Hai-yun1, Zhang Lei2, Wang Li-ming2, Zhao Wen-yu2, Yuan Qing2, Chen Yu2, Zhou Lin3, Zhang Ling-zhen3, Zhu You-hua2   

  1. 1Department of Urinary Surgery, the 94 Hospital of Chinese PLA, Nanchang  330002, Jiangxi Province, China; 2Organ Transplantation Center, 3Department of Laboratory Diagnosis, Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China
  • Received:2011-06-25 Revised:2011-08-25 Online:2011-12-31 Published:2011-12-31
  • Contact: Zhang Lei, Doctor, Attending physician, Organ Transplantation Center, Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China zl3514@hotmail.com
  • About author:Xiong Hai-yun☆, Doctor, Attending physician, Department of Urinary Surgery, the 94 Hospital of Chinese PLA, Nanchang 330006, Jiangxi Province, China jetxiongjet@hotmail. com

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摘要：

背景：临床上常以流式细胞检测受者外周血CD4、CD8细胞比值来揭示与排斥或感染相关的关系。
目的：探讨肾移植后排斥或感染时外周血CD4+及CD8+T细胞(简称CD4和CD8细胞)亚群计数的变化和意义。
方法：应用流式细胞仪检测肾移植121例受者CD4、CD8细胞数进行检测。根据入院病情将患者分为移植后正常组、急性排斥组、肺部感染组进行观察。
结果与结论：移植后正常患者和急性排斥患者相比，CD4、CD8细胞数差异均无显著性意义(P > 0.05)。肾移植后肺部感染患者CD4、CD8细胞数则均显著低于移植后正常组(P < 0.001)。当感染控制、症状改善时，CD4、CD8细胞数显著升高 (P < 0.001)。说明肾移植后CD4和CD8细胞计数可以作为免疫状态的参考，其对于感染的参考价值大于排斥，动态观察分析有助于指导治疗。

关键词: 细胞亚群, 肾移植, T细胞, 排斥反应, 感染

Abstract:

BACKGROUND: In the clinic, the ratio of peripheral blood CD4+ to CD8+ T cell subset counts determined by flow cytometry is often used to reveal the relationship related to rejection or infection.
OBJECTIVE: To investigate the value and significance of peripheral blood CD4+ and CD8+ T cell subset counts in patients suffering from rejection or infection after renal transplantation.
METHODS: CD4+ and CD8+ T cell subset counts in 121 patients receiving renal transplantation were determined by flow cytometry. According to disease condition, patients were assigned to normal group, acute rejection group and pulmonary infection group.
RESULTS AND CONCLUSION: There was no significant difference in CD4+ and CD8+ T cell subset counts between normal and acute rejection groups (P > 0.05). CD4+ and CD8+ T cell subset counts were significantly reduced in the pulmonary infection group than in the normal group (P < 0.001). When infection was controlled and symptoms were improved, CD4+ and CD8+ T cell subset counts were significantly increased (P < 0.001). These findings suggest that CD4+ and CD8+ T cell subset counts can be used as a reference of immune state in renal allograft recipients, they are more valuable in considering patients with infection than patients with rejection, and serial observation of CD4+ and CD8+ T cell subset counts may be very useful in optimizing treatment.

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