中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (40): 7520-7524.doi: 10.3969/j.issn.1673-8225.2011.40.025

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

尾静脉注射骨髓基质细胞和腹腔注射粒细胞集落刺激因子治疗大鼠脑梗死

顾  平1，2，张忠霞1，周美刚1，王彦永2，董  慈2，崔冬生1，耿  媛1，王铭维1，2   

  1. 1河北省脑老化与认知神经科学实验室，河北省石家庄市  050031
    2河北医科大学第一医院神经内科，河北省石家庄市  050031
  • 收稿日期:2011-03-11 修回日期:2011-04-19 出版日期:2011-10-01 发布日期:2011-10-01
  • 通讯作者: 王铭维，博士，主任医师，教授，博士生导师，河北省脑老化与认知神经科学实验室，河北医科大学第一医院神经内科，河北省石家庄市050031
  • 作者简介:顾平☆，女，1970年生，江苏省无锡市人，汉族，1993年河北医科大学毕业，博士，主任医师，教授，硕士生导师，主要从事神经病学方面的研究。 gpwh2000@yahoo.com.cn
  • 基金资助:

    河北省应用基础研究计划重点基础研究项目(08966102D)，课题名称：骨髓干细胞的不同方法移植治疗大鼠缺血性脑梗死疗效的比较研究。

Tail vein injection of bone marrow stromal cells versus intraperitoneal injection of granulocyte colony-stimulating factor in the treatment of cerebral infarction in rats

Gu Ping1, 2, Zhang Zhong-xia1, Zhou Mei-gang2, Wang Yan-yong1, 2, Dong Ci2, Cui Dong-sheng1, Geng Yuan1, Wang Ming-wei1, 2   

  1. 1Brain Aging and Cognitive Neuroscience Laboratory of Hebei Province, Shijiazhuang  050031, Hebei Province, China
    2Department of Neurology, First Hospital of Hebei Medical University, Shijiazhuang  050031, Hebei Province, China
  • Received:2011-03-11 Revised:2011-04-19 Online:2011-10-01 Published:2011-10-01
  • Contact: Wang Ming-wei, Doctor, Chief physician, Professor, Doctoral supervisor, Brain Aging and Cognitive Neuroscience Laboratory of Hebei Province, Shijiazhuang 050031, Hebei Province, China; Department of Neurology, First Hospital of Hebei Medical University, Shijiazhuang 050031, Hebei Province, China
  • About author:Gu Ping☆, Doctor, Chief physician, Professor, Master’s supervisor, Brain Aging and Cognitive Neuroscience Laboratory of Hebei Province, Shijiazhuang 050031, Hebei Province, China; Department of Neurology, First Hospital of Hebei Medical University, Shijiazhuang 050031, Hebei Province, China gpwh2000@yahoo.com.cn
  • Supported by:

    the Key Basic Research Project of Hebei Provincial Applied Basic Research Planning, No. 08966102D*

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摘要：

背景：骨髓间充质干细胞具有成神经分化特性，有很多试验也证实粒细胞集落刺激因子可以用于改善脑梗死后的神经功能。
目的：比较静脉移植骨髓间充质干细胞和腹腔注射粒细胞集落刺激因子动员干细胞来治疗大脑中动脉闭塞模型大鼠疗效。
方法：实验以改良的Zea-longa线栓法阻断大脑中动脉建立SD大鼠脑梗死模型，造模24 h后分别通过尾静脉注射骨髓间充质干细胞或腹腔注射粒细胞集落刺激因子。
结果与结论：两种治疗方法均可改善脑梗死模型大鼠的运动和认知功能，且粒细胞集落刺激因子对脑梗死模型大鼠的运动和认知功能的改善比尾静脉注射骨髓间充质干细胞明显，移植后第7，14天，粒细胞集落刺激因子组梗死面积小于骨髓间充质干细胞组(P < 0.05)，粒细胞集落刺激因子组BrdU阳性细胞数多于骨髓间充质干细胞组(P < 0.05)。提示粒细胞集落刺激因子动员骨髓干细胞治疗脑梗死的疗效可能优于骨髓间充质干细胞静脉注射的移植方法。

关键词: 骨髓基质细胞, 移植, 粒细胞集落刺激因子, 缺血性脑血管病, 中动脉闭塞模型

Abstract:

BACKGROUND: Bone marrow stem cells (BMSCs) possess the potency of transverse differentiation and the character of nerve formation. There are a few researches at intravenous BMSCs and intraperitoneal injection of granulocyte colony stimulating factor (G-CSF) to mobilize stem cells on treatment of cerebral artery occlusion model (MCAO) in rats.
OBJECTIVE: To compare the effects of tail vein injection of BMSCs versus intraperitoneal injection of G-CSF in the treatment of cerebral infarction in rats.
METHODS: In this experiment, modified Zea-longa suture method was applied on the SD rats to block the middle cerebral artery, thus to establish the model of cerebral infarction. BMSCs were injected via the tail vein and G-CSF was injected intraperitoneally at 24 hours after modeling.
RESULTS AND CONCLUSION: Cognitive and motor function could be improved by G-CSF of intraperitoneal injection and BMSCs of tail vein injection in rats. The improvement of cognitive and motor function of MCAO rats of G-CSF was significantly higher than that of BMSCs by the tail vein injection. At 7 and 14 days after transplantation, the infarct area in rats undergoing BMSCs transplantation was bigger than that of rats undergoing G-CSF (P < 0.05), and the number of BrdU positive cells in the G-CSF group was higher than that in the BMSCs group (P < 0.05). G-CSF can promote BMSCs to migrate to the corpus callosum. The results suggest that the method of G-CSF by mobilizing BMSCs may be better than BMSCs transplantation alone for treatment of cerebral ischemia.

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