中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (23): 4263-4266.doi: 10.3969/j.issn.1673-8225.2012.23.017

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

心肌及冠状动脉内移植骨髓单个核细胞对猪缺血心肌侧支血管的生成

孙 林，楚天舒，白文伟，汪丽琴，张 戈，阮志敏   

  1. 昆明医学院第二附属医院心血管内科，云南省心血管病研究所冠心病基础研究室，云南省昆明市 650101
  • 收稿日期:2011-11-17 修回日期:2012-02-11 出版日期:2012-06-03 发布日期:2013-11-06
  • 通讯作者: 阮志敏，副教授，昆明医学院第二附属医院心血管内科，云南省心血管病研究所冠心病基础研究室，云南省昆明市 650101 ruan_zhimin@126.com
  • 作者简介:孙林☆，女，1972年生，云南省宣威市人，汉族，2007年昆明医学院毕业，博士，副教授，主要从事冠心病临床及基础的研究。 sunlinkm@sina.com

Angiogenesis of autologous bone marrow mononuclear cells by intramyocardial and intracoronary transplantation into ischemic myocardium of a swine model of acute myocardial infarction

Sun Lin, Chu Tian-shu, Bai Wen-wei, Wang Li-qin, Zhang Ge, Ruan Zhi-min   

  1. Department of Cardiovascular Medicine, Second Affiliated Hospital of Kunming Medical College, Room for Coronary Heart Disease Research, Yunnan Institute of Cardiovascular Disease, Kunming 650101, Yunnan Province, China
  • Received:2011-11-17 Revised:2012-02-11 Online:2012-06-03 Published:2013-11-06
  • Contact: Ruan Zhi-min, Associate professor, Department of Cardiovascular Medicine, Second Affiliated Hospital of Kunming Medical College, Room for Coronary Heart Disease Research, Yunnan Institute of Cardiovascular Disease, Kunming 650101, Yunnan Province, China ruan_zhimin@126.com
  • About author:Sun Lin☆, M.D., Associate professor, Department of Cardiovascular Medicine, Second Affiliated Hospital of Kunming Medical College, Room for Coronary Heart Disease Research, Yunnan Institute of Cardiovascular Disease, Kunming 650101, Yunnan Province, China sunlinkm@sina.com

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摘要：

背景：目前有研究表明，心肌内直接注射骨髓单个核细胞可以使心肌梗死瘢痕区血管新生，改善缺血心肌血供。
目的：观察心肌内及冠状动脉内移植自体骨髓单个核细胞对猪急性心肌梗死后缺血心肌侧支血管生成的作用。
方法：22只小型猪制备急性心肌梗死模型后分为4组：心肌内移植组造模后即刻在缺血心肌内注射自体骨髓单个核细胞悬液；心肌内对照组同样方法即刻心肌内注射Hank’s平衡盐溶液；冠状动脉内移植组在造模后1周，左冠状动脉内注射自体骨髓单个核细胞悬液；冠状动脉对照组在造模后1周，同样方法左冠状动脉内注射Hank’s平衡盐溶液。
结果与结论：心肌内及冠状动脉内移植骨髓单个核细胞后1周，血清碱性成纤维细胞生长因子及血管内皮细胞生长因子水平差异无显著性意义，但明显高于各自对照组(P < 0.01)；移植后4周，心肌内移植组与冠状动脉内移植组小血管密度差异无显著性意义，但明显高于各自对照组(P < 0.01)；左室舒张末压差异无显著性意义，但明显低于各组对照组(P < 0.01)。提示心肌内及冠状动脉内移植骨髓单个核细胞均有助于促进猪缺血心肌血管新生及侧支循环形成。

关键词: 骨髓单个核细胞, 移植, 缺血心肌, 冠状动脉, 侧支循环, 猪

Abstract:

BACKGROUND: Current evidence exists that intramyocardial transplantation of bone marrow mononuclear cells can promote angiogenesis and blood supply in ischemic myocardium.
OBJECTIVE: To investigate the effects of intramyocardial and intracoronary transplantation of autologous bone marrow mononuclear cells into ischemic myocardium of a swine model of acute myocardial infarction.
METHODS: Twenty-two swine models of acute myocardial infarction were randomly divided into four groups. In the intramyocardial transplantation group, immediately after induction of acute myocardial infarction, autologous bone marrow mononuclear cell suspension was injected into the ischemic myocardium. In the intramyocardial transplantation control group, autologous bone marrow mononuclear cell suspension was replaced by Hank’s balanced salt solution. In the intracoronary transplantation group, at 1 week after induction of acute myocardial infarction, autologous bone marrow mononuclear cell suspension was injected into the left coronary artery. In the intracoronary transplantation control group, autologous bone marrow mononuclear cell suspension was replaced by Hank’s balanced salt solution.
RESULTS AND CONCLUSION: At 1 week after transplantation, there were no significant differences in serum levels of basic fibroblast growth factor and vascular endothelial growth factor between intramyocardial transplantation group and intracoronary transplantation group. The serum levels of basic fibroblast growth factor and vascular endothelial growth factor in intramyocardial transplantation group or intracoronary transplantation group were significantly higher than those in corresponding control group (P < 0.01). At 4 weeks after transplantation, there was no significant difference in blood vessel density between intramyocardial transplantation group and intracoronary transplantation group, but it was significantly higher than that in corresponding control group (P < 0.01). These finding suggest that intramyocardial and intracoronary transplantation of autologous bone marrow mononuclear cells can promote angiogenesis and collateral circulation formation in ischemic myocardium in the swines.

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