中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (8): 1409-1412.doi: 10.3969/j.issn.1673-8225.2011.08.019

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

体外自组装IKVAV纳米纤维凝胶在二维体系中对神经祖细胞分化的影响

扈佃磊1，于圣会1，刘  宁1，盛伟斌1，郭海龙1，马  艳2，毕晓娟2   

  1. 新疆医科大学第一附属医院，1脊柱外科，2医学研究中心，新疆维吾尔自治区乌鲁木齐市 830054
  • 收稿日期:2010-09-21 修回日期:2010-10-13 出版日期:2011-02-19 发布日期:2011-02-19
  • 通讯作者: 郭海龙，副主任医师，新疆医科大学第一附属医院脊柱外科，新疆维吾尔自治区乌鲁木齐市 830054 guohailong@tom.com
  • 作者简介:扈佃磊★，男，1986年生，山东省莒县人，汉族，新疆医科大学在读硕士，主要从事脊柱外科研究。 dianleihu@sina.com
  • 基金资助:

    新疆医科大学第一附属医院青年专项基金项目(20009QN06)。

Effect of self-assembling nanofiber gel containing IKVAV in two-dimensional culture system on differentiation of neural progenitor cells in vitro

Hu Dian-lei1, Yu Sheng-hui1, Liu Ning1, Sheng Wei-bin1, Guo Hai-long1, Ma Yan2, Bi Xiao-juan2   

  1. 1Department of Spinal Surgery, 2Medical Research Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi   830054, Xinjiang Uygur Autonomous Region, China
  • Received:2010-09-21 Revised:2010-10-13 Online:2011-02-19 Published:2011-02-19
  • Contact: Guo Hai-long, Associate chief physician, Department of Spinal Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China guohailong@tom.com
  • About author:Hu Dian-lei★, Studying for master’s degree, Department of Spinal Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China dianleihu@sina.com
  • Supported by:

    Youth Special Fund of First Affiliated Hospital of Xinjiang Medical University, No.2009N06*

PDF

484

被引次数

2

摘要：

背景：大量实验证明体外自组装的纳米支架材料可以促进神经祖细胞增殖与分化。
目的：观察体外自组装含IKVAV纳米纤维凝胶在二维体系中对神经祖细胞分化的影响。
方法：培养SD大鼠乳鼠神经祖细胞并用免疫荧光方法鉴定。在DMEM/F12触发下，含IKVAV的两亲性多肽溶液形成三维多孔凝胶，于透射电镜下观察其结构。将神经祖细胞分别接种到1%含IKVAV的两亲性多肽溶液及0.1 g/L多聚赖氨酸包被的盖玻片上，培养1，3，7 d后采用神经丝蛋白、胶质纤维酸性蛋白检测其分化情况。
结果与结论：培养出巢蛋白阳性细胞，并且能分化为神经丝蛋白阳性的神经元与胶质纤维酸性蛋白阳性的胶质细胞，证实为神经祖细胞；含IKVAV的两亲性多肽溶液自组形成凝胶，并在透射电镜下显示为纳米纤维，直径为7.0~8.0 nm，长度为100~1 500 nm； IKVAV的两亲性多肽溶液组向神经元分化得能力明显优于多聚赖氨酸组。说明体外自组装IKVAV纳米纤维凝胶在二维培养体系中对神经祖细胞分化有一定的促进作用。

关键词: 神经祖细胞, 纳米支架, IKVAV, 纳米纤维凝胶, 生物材料与纳米技术

Abstract:

BACKGROUND: Numerous studies have demonstrated that the self-assembling nanofiber promotes neural progenitor cells proliferation and differentiation. 
OBJECTIVE: To study the differentiation of neural progenitor cells on self-assembling nanofiber gel containing IKVAV in two-dimensional culture system in vitro.
METHODS: Neural progenitor cells of SD neonatal rats were cultured in culture medium and examined by immunofluorescence. Amphipathic polypeptide solution containing 1% IKVAV-PA was trigger to form three-dimensional porous gel by the addition of DMEM/F12, and the structure was detected under transmission electron microscope. Neural progenitor cells were seeded on the surface of coverslips covered with 1% KVAV-PA or covered with 0.1 g/L polylysine, and the cell differentiation was determined by neurofilament and glial fibrillary acidic protein after cultivated 1, 3, 7 days respectively.
RESULTS AND CONCLUSION: The cultured Nestin-positive cells were found, and they were able to differentiated into neurofilament-positive neurons and glial fibrillary acidic protein-positive glial cells, confirming as neural progenitor cells; Transmission electron microscopy showed that the gel derived from amphipathic polypeptide solution containing IKVAV-PA, was composed of nanofiber network, their diameter ranging 7.0-8.0 nm and the length 100-1 500 nm. The ability of differentiation into neurons of the amphipathic polypeptide solution containing IKVAV-PA surpasses to the polylysine. The self-assembling nanofiber gel contained IKVAV in two-dimensional culture system may promote the differentiation of neural progenitor cells in vitro.

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