中国组织工程研究

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

新型脊髓纳米组织工程支架的组织相容性

周继辉1,姚  猛2,王岩松2,隋福革1,刘玉刚2,赵丛然1,田飞鹏1   

  1. 1大庆龙南医院即齐齐哈尔医学院附属第五医院,黑龙江省大庆市  163453
    2哈尔滨医科大学附属第二医院脊柱外科,黑龙江省哈尔滨市  150086
  • 收稿日期:2012-10-09 修回日期:2012-11-16 出版日期:2013-05-21 发布日期:2013-05-21
  • 通讯作者: 姚猛,教授,哈尔滨医科大学附属第二医院脊柱外科,黑龙江省哈尔滨市 150086 王岩松,副教授,哈尔滨医科大学附属第二医院脊柱外科,黑龙江省哈尔滨市 150086
  • 作者简介:周继辉☆,男,1974年生,黑龙江省哈尔滨市人,汉族,2012年哈尔滨医科大学毕业,博士,副主任医师,主要从事脊髓组织工程研究。 ZHOUJIHUI321@163.com
  • 基金资助:

    国家自然科学基金项目(30800260);黑龙江省自然科学基金项目(D200916,D200902)。

Histocompatibility of new nano-scaffolds for spinal tissue engineering

Zhou Ji-hui1, Yao Meng2, Wang Yan-song2, Sui Fu-ge1, Liu Yu-gang2, Zhao Cong-ran1,  Tian Fei-peng1   

  1. 1 Longnan Hospital of Daqing, the Fifth Affiliated Hospital of Qiqihar Medical University, Daqing  163453, Heilongjiang Province, China
    2 Department of Spinal Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin  150086, Heilongjiang Province, China
  • Received:2012-10-09 Revised:2012-11-16 Online:2013-05-21 Published:2013-05-21
  • Contact: Yao Meng, Professor, Department of Spinal Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China Wang Yan-song, Associate professor, Department of Spinal Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
  • About author:Zhou Ji-hui☆, M.D., Associate chief physician, Longnan Hospital of Daqing, the Fifth Affiliated Hospital of Qiqihar Medical University, Daqing 163453, Heilongjiang Province, China ZHOUJIHUI321@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30800260; the Natural Science Foundation of Heilongjiang Province, No. D200916, D200902

摘要:

背景:纳米技术可改善脊髓组织工程生物材料的性能。
目的:分析新型脊髓纳米组织工程支架的组织相容性。
方法:以胶原为原料制备纤维定向排列及非定向排列的纳米纤维膜,培养及鉴定SD大鼠脊髓源性神经干细胞。将两种纳米纤维膜与SD乳鼠脊髓源性神经干细胞共培养,以正常培养的神经干细胞为对照,通过MTT实验检测纳米纤维膜的细胞相容性;以扫描电镜检测细胞在纳米纤维膜表面的黏附及增殖情况;将纳米纤维膜植入SD大鼠体内,通过组织学检查确定其降解情况及组织相容性;通过免疫组织化学实验确定神经干细胞在体内的存活及移动情况。
结果与结论:两种纳米纤维膜表面的神经干细胞黏附及增殖情况良好,MTT实验结果表明纳米纤维膜的细胞相容性佳,电镜结果表明细胞在纳米纤维膜表面黏附良好,增殖情况佳;在体内纳米纤维膜降解情况良好,组织相容性佳;BrdU标定的神经干细胞在SD大鼠体内存活并移动情况良好。结果表明新型纳米组织工程支架具有良好的细胞及组织相容性。

关键词: 生物材料, 纳米生物材料, 脊髓, 组织工程, 纳米支架, 纳米纤维膜, 组织相容性, 神经干细胞, 国家自然科学基

Abstract:

BACKGROUND: The properties of tissue engineering materials have been improved obviously with the rise and development of nanotechnology, the prospect of which is broad in spinal cord tissue engineering.
OBJECTIVE: To observe the histocompatibility of new nano-scaffolds for spinal tissue engineering.
METHODS: Electrospun aligned and randomly oriented nano?brous scaffolds were made of collagen. Spinal cord derived neural progenitor cells from Sprague-Dawley rats were cultured and identified, and then the cells were cultured on the aligned and randomly oriented collagen nano?brous scaffolds. Neural stem cells under normal culture served as controls. Scanning electron microscope was used to observe the cells’ adhesion and generation on the scaffold surface, and cell histocompatibility was determined with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Histocompatibility and degradation of nano?brous scaffolds in vivo were determined through histological observation. The survival and movement of neural stem cells in the body were determined by immunohistochemistry experiments.
RESULTS AND CONCLUSION: Superficial morphous of electrospun aligned and randomly oriented collagen nano?brous scaffolds were in accordence with contrivable requisition. The cell adhesion and proliferation on the surface of the collagen nano?brous scaffolds was perfect. Results from 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay showed that the cellular compatibility of nano-scaffolds was good. Nano?brous scaffolds degraded well and their histocompatibility in vivo was good. The 5-bromo-2'-deoxyuridine-labeled cells in vivo survived and migrated well. Histocompatibility of new nano-scaffolds for tissue engineering is satisfactory.

Key words: biomaterials, nanobiomaterials, spinal cord, tissue engineering, nano?brous scaffold, nanofibrous membrane, histocompatibility, neural stem cells, National Natural Science Foundation of China

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