中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (53): 9980-9986.doi: 10.3969/j.issn.2095-4344.2012.53.020

• 移植与免疫 transplantation and Immunology • 上一篇    下一篇

重组人白细胞介素10抗家兔皮肤移植排斥反应中Toll样受体的变

吕岩岩，孙万邦，李 佳，戴小波，张 磊，王胜香，陈富超，罗军敏   

  1. 遵义医学院珠海校区/贵州省免疫学研究生教育创新基地，广东省珠海市 519041
  • 收稿日期:2012-03-01 修回日期:2012-08-27 出版日期:2012-12-30 发布日期:2012-12-30
  • 通讯作者: 孙万邦，教授，遵义医学院珠海校区/贵州省免疫学研究生教育创新基地，广东省珠海市 519041 sunwb7224@sina.com
  • 作者简介:吕岩岩★，女，1984年生，山东省滕州人，汉族，2011年遵义医学院研究生毕业，硕士，主要从事临床免疫学的研究。 lvyan752@163.com

Changes of Toll like receptors in the reaction of recombinant human interleukin-10 resisting rabbit skin grafting

Lü Yan-yan, Sun Wan-bang, Li Jia, Dai Xiao-bo, Zhang Lei, Wang Sheng-xiang, Chen Fu-chao, Luo Jun-min   

  1. Zunyi Medical College Zhuhai Campus, Immunology Graduate Education and Innovation Base of Guizhou Province, Zhuhai 519041, Guangzhou Province, China
  • Received:2012-03-01 Revised:2012-08-27 Online:2012-12-30 Published:2012-12-30
  • Contact: Sun Wan-bang, Professor, Zunyi Medical College Zhuhai Campus, Immunology Graduate Education and Innovation Base of Guizhou Province, Zhuhai 519041, Guangzhou Province, China sunwb7224@sina.com
  • About author:Lü Yan-yan★, Master, Zunyi Medical College Zhuhai Campus, Immunology Graduate Education and Innovation Base of Guizhou Province, Zhuhai 519041, Guangzhou Province, China lvyan752@163.com

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摘要：

背景：现已认识到Toll样受体的活化与器官移植后排斥反应的关系十分密切，Toll样受体与移植免疫也逐渐成为研究的热点问题。
目的：观察重组人白细胞介素10抗皮肤移植排斥反应中受体兔血清Toll样受体的变化。
方法：制备兔背部缺损模型，将受体家兔分为6组，重组人白细胞介素10低、高剂量组、环孢素A低、高剂量组、联合用药组和生理盐水组，分别于移植前1 d开始肌肉注射5，10 μg/(kg•d)重组人白细胞介素10，5，10 mg/(kg•d)环孢素A，5 μg重组人白细胞介素10+5 mg环孢素A，1 mL/d生理盐水，连续用药10 d。
结果与结论：各组移植后Toll样受体4，7，8，9水平均较移植前升高，移植后14 d达到高峰。移植后4，7 d生理盐水组Toll样受体4水平高于联合用药组(P < 0.05)。移植后7 d生理盐水组Toll样受体7高于其他各组(P < 0.05)，Toll样受体8高于环孢素A高剂量组(P < 0.05)。各组移植不同时间点Toll样受体9水平差异无显著性意义(P > 0.05)。提示重组人白细胞介素10抗家兔皮肤移植反应中，Toll样受体4，7，8，9水平在皮肤移植后明显升高，当皮片排斥后达到高峰，之后逐渐下降，表明Toll样受体参与排斥反应过程。

关键词: 重组人白细胞介素10, 皮肤移植, 环孢素A, Toll样受体4, Toll样受体7, Toll样受体8, Toll样受体9, 兔, 免疫排斥

Abstract:

BACKGROUND: The researchers have recognized that there is a very close relationship between the activation of the Toll-like receptors and organ transplant rejection, and the Toll-like receptors and transplantation immunology have gradually become a hot topic.
OBJECTIVE: To observe the changes of Toll-like receptors in the serum drawing from rabbits treated with recombinant human interleukin-10 after allogeneic skin grafting.
METHODS: The rabbit back defect models were prepared, and the receptor rabbits were divided into six groups: low dose recombinant human interleukin-10 group, high dose recombinant human interleukin-10 group, low dose cyclosporine A group, high dose cyclosporine A group, joint group and normal saline group. The 5 and 10 μg/(kg•d) recombinant human interleukin-10, 5 and 10 mg/(kg•d) cyclosporine A, 5 μg recombinant human interleukin-10+5 mg cyclosporine A and 1 mL/d normal saline were injected into the rabbits respectively from 1 day before grafting and lasted for 10 days.
RESULTS AND CONCLUSION: The levels of serums Toll-like receptor 4, Toll-like receptor 7, Toll-like receptor 8 and Toll-like receptor 9 were increased after skin grafting compared with that before grafting and reached to the peak concentration on 14 days. At 4 and 7 days after grafting, the level of Toll-like receptors 4 in the normal saline group was higher than that in the joint group (P < 0.05). At 7 days after grafting, the level of Toll-like receptor 7 in the normal saline group was higher than that in the other five groups (P < 0.05), and the level of Toll-like receptor 8 in the normal saline group was higher than that in the high dose cyclosporine A group (P < 0.05). There was no significant difference of Toll-like receptor 9 level in each two groups at different time points after grafting (P > 0.05). The levels of serum Toll-like receptor 4, Toll-like receptor 7, Toll-like receptor 8 and Toll-like receptor 9 were increased obviously after the rabbits skin grafting which have treated with recombinant human interleukin-10 resisting skin grafting, and reached to the peak concentration after skin graft rejection, and then decreased gradually, which means Toll-like receptors participate in the allograft rejection.

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