中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (45): 8446-8449.doi: 10.3969/j.issn.1673-8225.2010.45.020

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

土鳖虫干预激素诱导骨髓间充质干细胞Ⅰ型胶原mRNA的表达

齐振熙1，李树强2，于  涛2   

  1. 1福建中西医结合研究院骨病研究所，福建省福州市  350108；2福建中医学院骨伤系，福建省福州市 350108
  • 出版日期:2010-11-05 发布日期:2010-11-05
  • 作者简介:齐振熙☆，男，1957年生，福建省福州市人，汉族，2004年福建中医学院毕业，博士，教授，主要从事骨缺血性坏死的研究。 zxqi@fjtcm.edu.cn
  • 基金资助:

    福建省科技计划项目(2007F5060)。

Intervention role of ground beetle on the expression of Type Ⅰ collagen mRNA in steroid-induced bone marrow mesenchymal stem cells

Qi Zhen-xi1, Li Shu-qiang2, Yu Tao2   

  1. 1 Institute of Osteopathy, Fujian Academy of Integrative Medicine, Fuzhou  350108, Fujian Province, China; 2 Department of Bone Injury, Fujian University of Traditional Chinese Medicine, Fuzhou  350108, Fujian Province, China
  • Online:2010-11-05 Published:2010-11-05
  • About author:Qi Zhen-xi☆, Doctor, Professor, Institute of Osteopathy, Fujian Academy of Integrative Medicine, Fuzhou 350108, Fujian Province, China zxqi@fjtcm.edu.cn
  • Supported by:

    the Science and Technology Project of Fujian Province, No. 2007F5060*

PDF

338

被引次数

7

摘要：

背景：研究表明激素性股骨头缺血性坏死的早期病理变化可能与激素诱导下骨髓间充质干细胞的成脂分化有关。
目的：观察土鳖虫含药血清对骨髓间充质干细胞成骨的促进作用。
方法：第3代骨髓间充质干细胞随机分为中药低剂量组、中药中剂量组、中药高剂量组、对照组和空白组。中药低、中、高剂量组分别加入用不同剂量含药血清配制的培养液(1 L培养基中含100 g含药血清)，同时加地塞米松至终浓度10-6 mol/L；对照组加入含生理盐水血清的培养液，同时加地塞米松至终浓度10-6 mol/L；空白组加入含生理盐水血清的培养液，不加地塞米松。检测干预6 d后各组细胞内成骨标志物Ⅰ型胶原mRNA及碱性磷酸酶的表达。
结果与结论：干预6 d后，细胞内碱性磷酸酶活性及Ⅰ型胶原 mRNA表达对照组较空白组明显降低，而中药各组较对照组均有不同程度的增高，且差异均具有显著性意义(P < 0.01)。说明土鳖虫防治激素性股骨头缺血坏死的机制不仅是改善股骨头的微循环，还与其抑制激素诱导下的骨髓间充质干细胞成骨分化减少有关。

关键词: 骨髓间充质干细胞, 股骨头坏死, 活血化瘀, 成骨分化, 土鳖虫

Abstract:

BACKGROUND: The early pathological changes of steroid-induced avascular necrosis of femoral head may be related to the steroid-induced bone marrow mesenchymal stem cells (BMSCs) adipogenic differentiation.
OBJECTIVE: To investigate the promoting effects of ground beetle drug-containing serum on osteogenic differentiation of BMSCs.
METHODS: The 3rd generation of BMSCs was randomly divided into blank group, control group and Chinese medicine with high, moderate and low dose groups. The low-, moderate- and low-dose Chinese medicine groups were treated with various doses of medium (1 L medium contained 100 g drug-containing serum), with an additional dexamethasone till final concentration was
10-6 mol/L. The control group was subjected to medium containing saline serum, with an additional dexamethasone till the final concentration was 10-6 mol/L. The medium containing saline serum was added in the blank group, without dexamethasone. At 6 days following intervention, ossify markers Collagen I mRNA and alkaline phosphatase (ALP) expression in cells was determined.
RESULTS AND CONCLUSION: Following 6 days of intervention, the intracellular ALP content and mRNA expression of collagen type I was significantly lower in the control group than the blank group, while was greater in the Chinese group than in the control group, and the differences were significant (P < 0.01). These suggest that mechanism of ground beetle to prevent and to cure the avascular necrosis of the femoral head is not only to improve the microcirculation, but also associates with the decrease in steroid-induced osteogenic differentiation of BMSCs.

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