中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (51): 9601-9604.doi: 10.3969/j.issn.1673-8225.2010.51.022

• 生物材料基础实验 basic experiments of biomaterials • 上一篇    下一篇

模拟构建人肝衰竭血清对体外培养肝细胞功能的影响

薛  琨1，高  森1，2，潘明新1，高  毅1   

  1. 1南方医科大学珠江医院再生医学研究所肝胆二科，广东省广州市 510282；2合肥市第二人民医院普外二科，安徽省合肥市 230000
  • 出版日期:2010-12-17 发布日期:2010-12-17
  • 通讯作者: 潘明新，博士，主任医师，南医科大学珠江医院肝胆二科，广东省广州市 510282 nfygaosen@sina.com
  • 作者简介:薛琨，男，1977年生，山西省太原市人，汉族，2001年浙江大学医学院毕业，主治医师，主要从事肝脏系统疾病方面的研究。 并列第一作者：高森★，男，1983年生，安徽省巢湖市人，汉族。2010年南方医科大学毕业，硕士，医师，主要从事生物人工肝方面的研究。nfygaosen@sina.com
  • 基金资助:

    国家高技术研究与发展计划(863计划)(2006AA02A141)，人源细胞混合性人工肝的研制与开发。

Effect of human hepatic failure serum on function of hepatocytes cultured in vitro: A simulation experiment

Xue Kun1, Gao Sen 1,2, Pan Ming-xin1, Gao Yi1   

  1. 1 Second Hepatobiliary Division, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou   510282, Guangdong Province, China; 2 Second Department of General Surgery, Second People’s Hospital of Hefei, Hefei  230000, Anhui Province, China
  • Online:2010-12-17 Published:2010-12-17
  • Contact: Pan Ming-xin, Doctor, Chief physician, Second Hepatobiliary Division, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China nfygaosen@sina.com
  • About author:Xue Kun, Attending physician, Second Hepatobiliary Division, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China Gao Sen★, Master, Physician, Second Hepatobiliary Division, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China; Second Department of General Surgery, Second People’s Hospital of Hefei, Hefei 230000, Anhui Province, China nfygaosen@sina.com
  • Supported by:

    the National High-Tech Research and Development Program of China (863 Program), No. 2006AA02A141*

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摘要：

背景：生物人工肝核心材料为肝细胞，其功能好坏直接影响临床效果。
目的：模拟构建一种能够和肝衰竭血清对体外培养肝细胞具有相同生物学效应的组合物。
方法：观察和对比模拟肝衰竭血清、人肝衰竭血清和体积分数10%胎牛血清对培养的CL-1细胞形态学和细胞活力的变化，检测CL-1细胞酶包括乳酸脱氢酶、谷丙转氨酶、谷草转氨酶及谷胱甘肽的分泌量。
结果与结论：模拟肝衰竭血清和人肝衰竭血清对培养的CL-1细胞活力，乳酸脱氢酶、谷丙转氨酶、谷草转氨酶和谷胱甘肽的分泌量的影响差异无显著性意义(P > 0.05)，CL-1细胞的生长均受到抑制，体积分数10%胎牛血清细胞功能良好。结果提示，模拟肝衰竭血清组合物与人肝衰竭血清对CL-1细胞的生物学效应基本相同。

关键词: 肝衰竭, CL-1细胞, 血清, 体外功能, 人工肝

Abstract:

BACKGROUND: The key material of bioartificial liver is the hepatocytes, whose function directly affects the clinical results.
OBJECTIVE: To simulate a composite with similar biological effects with hepatic failure serum on the hepatocytes function cultured in vitro.
METHODS: The efficacy of the simulated serum liver failure, human liver failure serum and 10% fetal bovine serum on the cultured CL-1 cells was observed and compared, including cell morphology and cell viability, lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase and glutathione secretion.
RESULTS AND CONCLUSION: The simulation liver failure and human liver failure serum had the same efficacy on CL-1 cell viability, lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase and glutathione secretion, with no significant difference (P < 0.05), CL-2 cell growth was inhibited, and CL-1 cells had good function state in 10% fetal bovine serum. The simulated liver failure serum and human liver failure serum have the same biological effect on CL-1 cells.

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