中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (18): 3297-3300.doi: 10.3969/j.issn.1673-8225.2010.18.016

• 细胞与组织移植 cell and tissue transplantation • 上一篇    下一篇

移植坐骨神经大鼠相应背根神经节中生长相关蛋白43的表达

赵东波，孙鸿斌，李  强，崔树森   

  1. 吉林大学中日联谊医院手外科，吉林省长春市  130033
  • 出版日期:2010-04-30 发布日期:2010-04-30
  • 通讯作者: 崔树森，教授，博士生导师，吉林大学中日联谊医院手外科，吉林省长春市130033 sscui916@126.com
  • 作者简介:赵东波☆，男，1972年生，黑龙江省绥化市人，汉族，吉林大学中日联谊医院在读博士，主要从事周围神经损伤与再生研究。 zdbzhs@126.com

Expression of growth associated protein 43 at the corresponding dorsal root ganglia following sciatic nerve transplantation in rats

Zhao Dong-bo, Sun Hong-bin, Li Qiang, Cui Shu-sen   

  1. Department of Hand Surgery, China-Japan Union Hospital, Jilin University, Changchun  130033, Jilin Province, China
  • Online:2010-04-30 Published:2010-04-30
  • Contact: Cui Shu-sen, Professor, Doctoral supervisor, Department of Hand Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China sscui916@126.com
  • About author:Zhao Dong-bo☆, Studying for doctorate, Department of Hand Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China zdbzhs@126.com

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463

被引次数

3

摘要：

背景：周围神经移植后，相应的背根神经节感觉神经元胞体内生长相关蛋白43(GAP-43)表达的时程与规律尚未明确。
目的：观察大鼠坐骨神经移植术后GAP-43在相应背根神经节中的表达变化。
方法：取健康成年雄性Wistar大鼠，随机分为2组，对照组行假手术组；实验组行坐骨神经移植，分别于术后3 d，1，2，4，6，8周6个时间点处死后取其手术侧L4~L5背根神经节，应用RT-PCR和Western Blot技术检测GAP-43 mRNA及其蛋白表达。
结果与结论：对照组大鼠背根神经节中GAP-43 mRNA表达量较低，且随时间无明显改变；实验组术后1周时GAP-43 mRNA在相应背根神经节中即有表达增强，2周时达到高峰，6~8周表达逐渐减弱。两组大鼠背根神经节中GAP-43蛋白与GAP-43 mRNA表达的变化规律相同。结果表明神经损伤后相应神经元的再生能力存在损伤反应性变化。

关键词: 背根神经节, GAP-43, 周围神经, 损伤, 大鼠, 神经移植

Abstract:

BACKGROUND: Following peripheral nerve transplantation, the time phases and law of growth associated protein 43 (GAP-43) expression in sensory neuron soma of the corresponding dorsal root ganglia remain unclear.
OBJECTIVE: To investigate the expression of GAP-43 at the corresponding dorsal root ganglia following sciatic nerve transplantation in rats.
METHODS: Adult male Wistar rats were randomly divided into 2 groups: control group (sham-operated group) and experimental group (nerve transplantation group). The rats were killed at 3 days, 1, 2, 4, 6, and 8 weeks after operation and the dorsal root ganglia L4-5 of every rat was taken for observation. The expression of GAP-43 mRNA and protein in the corresponding segments of spinal cord were detected by RT-PCR and Western-blot techniques.
RESULTS AND CONCLUSION: GAP-43 mRNA showed a low level of expression in the control group, which had no obvious changes in different phases. In the experimental group, GAP-43 mRNA expression was observed remarkably in the dorsal root ganglia at the first week, reached a peak at the second week and gradually descended from the sixth week. The GAP-43 protein expression showed the same laws as that of GAP-43 mRNA. The results demonstrated that there exists damage reactivity change in the regeneration ability of neurons.

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