中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (19): 3539-3543.doi: 10.3969/j.issn.1673-8225.2010.19.024

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

桃红四物汤对激素诱导骨髓间充质干细胞成脂分化的干预作用

李树强1,于 涛1,齐振熙2   

  1. 1福建中医学院骨伤系,福建省福州市  350108;
    2福建中医学院中西医结合研究院骨病研究所,福建省福州市  350108
  • 出版日期:2010-05-07 发布日期:2010-05-07
  • 通讯作者: 齐振熙,博士,教授,博士生导师,福建中医学院中西医结合研究院骨病研究所,福建省福州市 350108 zxqi@fjtcm.edu.cn
  • 作者简介:李树强,男,河南省太康县人,汉族,福建中医学院骨伤系毕业,硕士,主要从事股骨头缺血坏死的研究。 lshq921@yahoo.com.cn
  • 基金资助:

    陈可冀中西医结合发展基金项目(CKJ2007006)。

Intervention of Taohong Siwu Decoction on adipogenic differentiation of steroid-induced bone marrow mesenchymal stem cells

Li Shu-qiang1, Yu Tao1, Qi Zhen-xi2   

  1. 1Department of Orthopaedics and Traumatology, Fujian Traditional Chinese Medical College, Fuzhou  350108, Fujian Province, China;
    2Institute of Bone Disease, Fujian Traditional Chinese Medical College Academy of Integrated Traditional and Western Medicine, Fuzhou  350108, Fujian Province, China
  • Online:2010-05-07 Published:2010-05-07
  • Contact: Qi Zhen-xi, Doctor, Professor, Doctoral supervisor, Institute of Bone Disease, Fujian Traditional Chinese Medical College Academy of Integrated Traditional and Western Medicine, Fuzhou 350108, Fujian Province, China zxqi@fjtcm.edu.cn
  • About author:Li Shu-qiang, Master, Department of Orthopaedics and Traumatology, Fujian Traditional Chinese Medical College, Fuzhou 350108, Fujian Province, China lshq921@yahoo.com. cn
  • Supported by:

    the Development Fund for Integrative Medicine of Chen Keji, No. CKJ2007006*

摘要:

背景:临床组织学及病理学研究证明:激素性股骨头缺血性坏死的早期病理变化是股骨头骨髓内造血组织减少,脂肪组织增多,这可能与激素诱导下骨髓间充质干细胞的成脂分化有关。目前被很多学者用来解释激素性股骨头缺血性坏死的发病机制。
目的:观察桃红四物汤对激素诱导骨髓间充质干细胞成脂分化的干预作用。
方法:体外培养大鼠骨髓间充质干细胞,通过大剂量激素诱导体外培养的骨髓间充质干细胞成脂分化,在诱导成脂的同时给予桃红四物汤含药血清干预。检测干预6 d后细胞内成脂标志物三酰甘油、PPARγ mRNA和aP2 mRNA的表达。
结果与结论:桃红四物汤含药血清可对抗激素诱导下的骨髓间充质干细胞三酰甘油、PPARγ mRNA和aP2 mRNA表达的增加。提示桃红四物汤防治激素性股骨头缺血坏死的机制不仅是改善股骨头的微循环,同时还与其抑制激素诱导下的骨髓间充质干细胞成脂分化有关。

关键词: 桃红四物汤, 骨髓间充质干细胞, 股骨头坏死, 诱导, 分化, 大鼠

Abstract:

BACKGROUND: Clinical pathological and histological research has verified that the early pathological changes of steroid-induced avascular necrosis of femoral head are reduced bone marrow hematopoietic tissue and increased adipose tissue in femoral head and which may be related to adipogenic differentiation of steroid-induced bone marrow mesenchymal stem cells (BMSCs). These pathological changes are currently being used to explain the pathogenesis of steroid-induced avascular necrosis of the femoral head.
OBJECTIVE: To investigate effects of Taohong Siwu Decoction on adipogenic differentiation of steroid-induced BMSCs.
METHODS: Rat BMSCs were cultured in vitro. High-dose steroid was used to induce adipogenic differentiation of BMSCs in vitro, at the same time, Taohong Siwu Decoction containing serum was given. After 6 days of intervention, adipogenic markers triglyceride, PPARγ mRNA and aP2 mRNA expression were determined.
RESULTS AND CONCLUSION: Taohong Siwu Decoction containing serum could resist triglyceride, PPARγ mRNA and aP2 mRNA expression in steroid-induced BMSCs. These indicated that mechanism of Taohong Siwu Decoction to prevent and cure the avascular necrosis of the femoral head is not only to improve the microcirculation, but also is associated with inhibitory effects on steroid-induced adipogenic differentiation of BMSCs.

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