中国组织工程研究

中国组织工程研究 ›› 2024, Vol. 28 ›› Issue (26): 4113-4120.doi: 10.12307/2024.393

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

有氧运动训练影响阿尔茨海默症小鼠海马Notch1、Caspase-3的表达

杨力源1，张业廷2，李垂坤1，魏翠兰3   

  1. 1成都大学体育学院，四川省成都市  610106；2中国民用航空飞行学院，四川省广汉市  618307；3成都理工大学体育学院，四川省成都市  610059
  • 收稿日期:2023-06-06 接受日期:2023-07-11 出版日期:2024-09-18 发布日期:2023-09-28
  • 通讯作者: 张业廷，博士，讲师，中国民用航空飞行学院，四川省广汉市 618307
  • 作者简介:杨力源，男，1989年生，江苏省泰州市人，汉族，2014年成都体育学院毕业，硕士，实验师，主要从事体质健康促进等研究。
  • 基金资助:
    四川省科技计划项目(2020YFH0184)，项目负责人：张业廷；中央高校基本科研业务费专项资金资助(PHD2023-003)，项目负责人：张业廷

Effects of aerobic exercise on the expression of Notch1 and Caspase-3 in the hippocampus of Alzheimer’s disease mice

Yang Liyuan1, Zhang Yeting2, Li Chuikun1, Wei Cuilan3   

  1. 1College of Physical Education, Chengdu University, Chengdu 610106, Sichuan Province, China; 2Civil Aviation Flight University of China, Guanghan 618307, Sichuan Province, China; 3Sports Institute of Chengdu University of Technology, Chengdu 610059, Sichuan Province, China
  • Received:2023-06-06 Accepted:2023-07-11 Online:2024-09-18 Published:2023-09-28
  • Contact: Zhang Yeting, PhD, Lecturer, Civil Aviation Flight University of China, Guanghan 618307, Sichuan Province, China
  • About author:Yang Liyuan, Master, Experimentalist, College of Physical Education, Chengdu University, Chengdu 610106, Sichuan Province, China
  • Supported by:
    Sichuan Provincial Science and Technology Program, No. 2020YFH0184 (to ZYT); the Fundamental Research Fund for the Central Universities, No. PHD2023-003 (to ZYT)

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摘要：


文题释义：

阿尔茨海默症：是最常见的神经退行性疾病，是痴呆最主要的类型(60%-80%)，临床表现为起病隐匿、缓慢进展、逐渐加重的特点，会出现学习与记忆等认知功能障碍、精神行为异常等症状。缺乏身体锻炼是导致阿尔茨海默症发生的危险因素之一。目前，人体试验和动物实验均表明体育运动可预防阿尔茨海默症的发生或延缓其病程。
有氧运动：是指主要以有氧代谢提供运动中所需能量的运动方式。有氧运动能锻炼心、肺功能，使心血管系统能更有效、快速地把氧传输到身体的每一个部位。长期坚持有氧运动能增加体内血红蛋白的数量，提高机体抵抗力，抗衰老，增强大脑皮质的工作效率和心肺功能，增加脂肪消耗，防止动脉硬化，降低心脑血管疾病的发病率。


背景：β-淀粉样蛋白和Tau蛋白会对阿尔茨海默症患者的认知功能产生不良影响，研究发现Notch1及Caspase-3能够调控β-淀粉样蛋白和Tau蛋白的表达。Notch1及Caspase-3是否介导了有氧运动改善阿尔茨海默症患者认知能力的过程还不清楚，目前缺乏长期有氧运动影响阿尔茨海默症小鼠海马中Notch1及Caspase-3表达的研究。 

目的：观察长期有氧运动干预阿尔茨海默症小鼠的空间学习记忆情况及其海马中Notch1及Caspase-3的表达，探讨Notch1及Caspase-3对阿尔茨海默症小鼠的影响。
方法：将3月龄野生型及APP/PS1双转基因阿尔茨海默症小鼠随机分为4组：野生对照组、野生运动组、阿尔茨海默症对照组、阿尔茨海默症运动组，每组20只。对照组小鼠不进行运动，运动组小鼠进行5个月的有氧运动干预。运动干预结束后，采用Morris水迷宫检测小鼠空间学习记忆能力；采用Real-time PCR、免疫荧光及Western blot检测各组小鼠海马组织Aβ1-42、Tau、Notch1及Caspase-3蛋白的表达。

结果与结论：①阿尔茨海默症小鼠空间学习记忆能力显著差于野生组(P < 0.05)；运动组小鼠空间学习记忆能力显著优于对照组(P < 0.05)；②阿尔茨海默症对照组小鼠海马Aβ1-42、Tau、Notch1及Caspase-3表达均显著高于野生对照组(P < 0.05)；阿尔茨海默症运动组小鼠海马Aβ1-42、Tau、Notch1及Caspase-3表达显著低于阿尔茨海默症对照组(P < 0.05)；③提示：长期有氧运动干预能够改善阿尔茨海默症小鼠的空间学习记忆能力，而这可能与有氧运动降低阿尔茨海默症小鼠海马Notch1、Caspase-3、Aβ1-42及Tau蛋白表达有关。

https://orcid.org/0000-0003-0031-2891(张业廷)

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 阿尔茨海默症, 有氧运动, 学习记忆能力, Notch1, Caspase-3

Abstract: BACKGROUND: β-amyloid protein and Tau protein have adverse effects on the cognitive function of Alzheimer’s disease patients, and Notch1 and Caspase-3 can regulate the expression of β-amyloid protein and Tau protein. It is not clear whether Notch1 and Caspase-3 mediate the process of aerobic exercise to improve the cognitive ability of Alzheimer’s disease patients. At present, there is a lack of studies on the effect of long-term aerobic exercise on the expression of Notch1 and Caspase-3 in the hippocampus of Alzheimer’s disease mice.
OBJECTIVE: To observe the expression of Notch1 and Caspase-3 in the hippocampus of Alzheimer’s disease mice undergoing long-term aerobic exercise and to investigate the effects of Notch1 and Caspase-3 in Alzheimer’s disease mice.
METHODS: Wild type and APP/PS1 double-transgenic Alzheimer’s disease mice aged 3 months were randomly divided into four groups: wild control group, wild exercise group, Alzheimer’s disease control group and Alzheimer’s disease exercise group, with 20 mice in each group. Mice in the control groups were not subjected to exercise, while those in the exercise groups received aerobic exercise intervention for 5 months. After the exercise intervention, Morris water maze was used to detect the spatial learning and memory ability of mice. Real-time PCR, immunofluorescence and western blot were used to detect the expressions of Aβ1-42, Tau, Notch1 and Caspase-3 in the hippocampal tissues of mice in each group.
RESULTS AND CONCLUSION: The spatial learning and memory ability of Alzheimer’s mice was significantly worse than that of wild-type mice (P < 0.05). The spatial learning and memory ability of mice in the exercise groups were significantly better than that in the corresponding control groups (P < 0.05). The expressions of Aβ1-42, Tau, Notch1 and Caspase-3 in the hippocampus were significantly higher in the Alzheimer’s disease control group than the wild control group (P < 0.05) and were significantly lower in the Alzheimer’s disease exercise group than the Alzheimer’s disease control group (P < 0.05). To conclude, long-term aerobic exercise can improve the spatial learning and memory ability of Alzheimer’s disease mice, which may be related to the decreased expression of Notch1, Caspase-3, Aβ1-42 and Tau protein in the hippocampus of Alzheimer’s disease mice.

Key words: Alzheimer’s disease, aerobic exercise, learning and memory ability, Notch1, Caspase-3

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