中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (12): 1931-1936.doi: 10.12307/2022.519

• 骨与关节综述 bone and joint review • 上一篇    下一篇

Micro CT对标本三维立体重建优势及在骨关节炎动物模型中的应用

杜龙龙1,袁普卫1,杨  威2,李雪枫1,高启萌1   

  1. 1陕西中医药大学,陕西省咸阳市   712046;2广州中医药大学,广东省广州市   510006
  • 收稿日期:2021-06-26 修回日期:2021-07-08 接受日期:2021-09-04 出版日期:2022-04-28 发布日期:2021-12-15
  • 通讯作者: 袁普卫,博士,教授,主任医师,陕西中医药大学,陕西省咸阳市 712046
  • 作者简介:杜龙龙,男,1993年生,陕西省延安人市,在读硕士,主要从事骨与关节退行性疾病研究。
  • 基金资助:
    长安医学关中李氏骨伤学术流派传承工作室建设项目(陕中医药发 [2018] 40 号),项目负责人:袁普卫;陕西省骨退行性疾病中西医结合防治重点研究室建设项目(陕中医药发 [2018] 32 号),项目负责人:袁普卫;咸阳市重点实验室建设项目(2019k01-53),项目负责人:袁普卫

Advantages of micro CT in three-dimensional reconstruction of specimens and its application in animal models of osteoarthritis

Du Longlong1, Yuan Puwei1, Yang Wei2, Li Xuefeng1, Gao Qimeng1   

  1. 1Shaanxi University of Chinese Medicine, Xianyang 712046, Shaanxi Province, China; 2Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong Province, China
  • Received:2021-06-26 Revised:2021-07-08 Accepted:2021-09-04 Online:2022-04-28 Published:2021-12-15
  • Contact: Yuan Puwei, MD, Professor, Chief physician, Shaanxi University of Chinese Medicine, Xianyang 712046, Shaanxi Province, China
  • About author:Du Longlong, Master candidate, Shaanxi University of Chinese Medicine, Xianyang 712046, Shaanxi Province, China
  • Supported by:
    the Li’s Orthopedic Inheritance Studio Construction Project of Chang’an Medicine, No. [2018] 40 (to YPW); Shaanxi Provincial Key Laboratory of Bone Degenerative Disease Prevention and Treatment of Integrated Traditional Chinese and Western Medicine Construction Project, No. [2018] 32 (to YPW); Xianyang Key Laboratory Construction Project, No. 2019k01-53 (to YPW)

摘要:

文题释义:
Micro CT:也称为显微CT,它是采用了微焦点X射线球管,对活体小动物或硬、软组织标本进行扫描成像分析的技术,它的分辨率高达2.5 μm。可以动态分析活体动物体内相关组织的形态特征,在对样本扫描的基础上,进行组织三维重建、骨形态学分析等,同时可通过软件进行3D图像高级处理、力学分析等相关分析。
骨关节炎:是常见的骨退行性疾病,会导致全关节组织的病理改变,后期会有致残风险,具体机制未明,是目前研究的热点问题。

背景:骨关节炎的病理进程中有多种因素共同作用,包括滑膜炎症、软骨丢失、软骨下骨损害、骨质增生等,其具体机制尚未明确。Micro CT是一种非破坏性成像工具,可以形成样本的高分辨率3D图像,实现骨和软骨的相关结构和功能的研究、疾病进展和治疗的定量评估及对比增强软组织成像。通过micro CT扫描骨关节炎动物模型,可以明确在骨关节炎病程中关节、关节软骨、软骨下骨、滑膜、血管等组织的病理变化,有利于骨关节炎病理进展的研究。
目的:归纳总结micro CT在骨关节炎动物模型的关节、软骨、软骨下骨、滑膜、骨内血管的成像方法及研究展望,为骨关节炎的病理机制变化研究提供新的方法。
方法:通过检索中国知网、Web of Science、Embase、PubMed、万方等数据库,英文关键词为“Osteoarthritis,micro CT,Pathology”等,中文关键词为“骨关节炎,micro CT,病理”等,选取相关性强,发表年限较新的合格文献进行综述。
结果与结论:①Micro CT在关节、软骨、软骨下骨、骨内血管都有较好的成像表现,成像方法成熟,改变传统病理切片破坏组织整体性、步骤繁琐的缺点。②Micro CT在滑膜的成像方面尚未有文献报道,但在如脂肪、肿瘤等软组织的成像中有着不错的表现,对滑膜的成像在未来有研究前景。③Micro CT可以对标本进行三维立体重建,打破常规二维病理切片的局限性,为骨关节炎的病理机制研究提供新的模式。

https://orcid.org/0000-0002-9729-9576 (杜龙龙) 

中国组织工程研究杂志出版内容重点:人工关节;骨植入物;脊柱;骨折;内固定;数字化骨科;组织工程

关键词: 骨关节炎, Micro CT, 动物模型, 病理, 软骨, 软骨下骨, 骨内血管, 综述

Abstract: BACKGROUND: There are many factors in the pathological process of osteoarthritis, including synovial inflammation, cartilage loss, subchondral bone damage, bone hyperplasia and so on. The specific mechanism is not yet clear. Micro CT is a non-destructive imaging tool, which can form high-resolution 3D images of samples, and realize the research of related structures and functions of bone and cartilage, quantitative evaluation of disease progression and treatment, and contrast-enhanced soft tissue imaging. Micro CT scanning of osteoarthritis animal model can clarify the pathological changes of joints, articular cartilage, subchondral bone, synovium, blood vessels and other tissues in the course of osteoarthritis, which is conducive to the study of the pathological progress of osteoarthritis.
OBJECTIVE: To summarize the imaging methods and research prospects of micro CT in joints, cartilage, subchondral bone, synovium and intraosseous vessels of osteoarthritis animal model, so as to provide a new method for the study of the pathological mechanism of osteoarthritis.
METHODS: By searching the databases of CNKI, Web of Science, Embase, PubMed and Wanfang, the Chinese and English keywords were “osteoarthritis, micro CT, pathology”. The qualified articles with strong correlation and relatively new publication years were selected for review. 
RESULTS AND CONCLUSION: (1) Micro CT has good imaging performance in joints, cartilage, subchondral bone, and intraosseous vessels. The imaging method is mature, which changes the shortcomings of traditional pathological sections on destroying tissue integrity and cumbersome steps. (2) Micro CT has not been reported in the literature on the imaging of synovium, but it has a good performance in the imaging of soft tissues, such as fat and tumor. The imaging of synovium has a research prospect in the future. (3) Micro CT can carry out three-dimensional reconstruction of specimens, break through the limitations of conventional two-dimensional pathological sections, and provide a new model for the study of the pathological mechanism of osteoarthritis.

Key words: osteoarthritis, micro CT, animal model, pathology, cartilage, subchondral bone, intraosseous blood vessel, review

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