中国组织工程研究

中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (4): 540-545.doi: 10.12307/2022.089

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

聚醚醚酮、二氧化锆和纯钛基台材料表面口腔微生物黏附的对比

邱  鹏1，2，傅琪淋3，刘  敏1，2，兰玉燕1，2，王  频1，2   

  1. 1西南医科大学口颌面修复重建与再生实验室，四川省泸州市   646000；2西南医科大学附属口腔医院修复科，四川省泸州市   646000；3绵阳市第三人民医院口腔科，四川省绵阳市   621000
  • 收稿日期:2020-10-12 修回日期:2020-10-14 接受日期:2020-11-21 出版日期:2022-02-08 发布日期:2021-11-03
  • 通讯作者: 王频，硕士，主治医师，西南医科大学口颌面修复重建与再生实验室，四川省泸州市 646000；西南医科大学附属口腔医院修复科，四川省泸州市 646000
  • 作者简介:邱鹏，男，1995年生，四川省泸州市人，汉族，西南医科大学在读硕士，主要从事口腔颌面部组织修复重建与再生研究。
  • 基金资助:
    四川省科技厅-泸州市科技局-泸州医学院联合项目(LY-51)，项目负责人：刘敏；四川省医学科研课题计划(S19023)，项目负责人：刘敏；泸州市科技局[2015-S-50，(1/3)]，项目负责人：兰玉燕；四川省教育厅四川医科大学大学生创新创业项目(2015130)，项目负责人：兰玉燕；四川医科大学校级教育教学改革项目(JG2015055)，项目负责人：兰玉燕

Comparison of oral micro-adhesion on polyetheretherketone, zirconium dioxide, and pure titanium abutment

Qiu Peng1, 2, Fu Qilin3, Liu Min1, 2, Lan Yuyan1, 2, Wang Pin1, 2   

  1. 1Oral & Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, Sichuan Province, China; 2Department of Prosthodontics, Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China; 3Department of Stomatology, The Third Hospital of Mianyang, Mianyang 621000, Sichuan Province, China
  • Received:2020-10-12 Revised:2020-10-14 Accepted:2020-11-21 Online:2022-02-08 Published:2021-11-03
  • Contact: Wang Pin, Master, Attending physician, Oral & Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Department of Prosthodontics, Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • About author:Qiu Peng, Master candidate, Oral & Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Department of Prosthodontics, Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Supported by:
    the Joint Project of Luzhou science and Technology Bureau Luzhou Medical College of Science and Technology Department of Sichuan Province, No. LY-51 (to LM); the Medical Research Project of Sichuan Province, No. S19023 (to LM); Luzhou Science and Technology Bureau, No. [2015-S-50, (1/3)] (to LYY); Innovation and Entrepreneurship Project of Sichuan Medical University of Department of Education of Sichuan Province, No. 2015130 (to LYY); School-Level Education and Teaching Reform Project of Sichuan Medical University, No. JG2015055 (to LYY)

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摘要：

文题释义：
聚醚醚酮：为一种全芳香族半结晶热塑性工程塑料，颜色与牙体组织相似，弹性模量与颌骨皮质骨相近。其次，聚醚醚酮还具有良好的化学稳定性、热稳定性、抗蠕变性，并且易于加工。聚醚醚酮及其复合材料已被广泛应用于军事、航空航天、汽车、医疗领域，在口腔材料学领域也有一定的应用。
二氧化锆：为锆的主要氧化物，具有良好半透光性、美学性能和生物相容性，在前牙区美学种植修复中有着重要的地位。
纯钛：具有良好的生物相容性、机械性能和化学稳定性，在口腔种植修复中被广泛应用为种植体和基台材料，且表现出良好的临床效果。
背景：二氧化锆和纯钛基台在前牙区应用中出现了一些美学和生物力学问题，一种符合生物力学的美学基台材料亟待被研究。
目的：比较牙龈卟啉单胞菌及全唾液在聚醚醚酮、二氧化锆、纯钛基台材料表面的初期黏附情况。
方法：将聚醚醚酮、二氧化锆、纯钛制成圆片形试件，利用扫描电镜观察表面形态特征，原子力显微镜测量试件表面粗糙度，接触角测量仪测量接触角。采用原子力显微镜探针测量牙龈卟啉单胞菌在3种试件表面的黏附力，采用扫描电镜和CCK-8法评估牙龈卟啉单胞菌及全唾液在3种试件表面的初期黏附量。
结果与结论：①扫描电镜显示，聚醚醚酮、纯钛试件表面呈沟槽状，且聚醚醚酮的沟槽多于纯钛，二氧化锆表面可见少量微孔；原子力显微镜显示，3种试件表面均可见平行的沟槽状起伏，聚醚醚酮和二氧化锆的沟槽峰谷结构较纯钛突出，且二氧化锆表面的类孔状凹凸结构较聚醚醚酮表面多；②二氧化锆的表面粗糙度大于纯钛(P < 0.05)，其余组间两两比较差异无显著性意义(P > 0.05)；3组试件的接触角比较差异无显著性意义(P > 0.05)；③牙龈卟啉单胞菌在聚醚醚酮表面的黏附力低于二氧化锆和纯钛(P < 0.05)；④扫描电镜显示，聚醚醚酮表面的细菌黏附量最少，而纯钛表面最多；CCK-8检测显示，聚醚醚酮表面的初期细菌黏附量低于二氧化锆和纯钛(P < 0.05)；⑤结果表明，在抑制细菌初期黏附方面，聚醚醚酮较传统基台材料二氧化锆、纯钛更具优势。

https://orcid.org/0000-0002-0523-2332 (邱鹏) 

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 聚醚醚酮, 二氧化锆, 纯钛, 细菌黏附, 种植义齿, 基台材料

Abstract: BACKGROUND: There are some aesthetic and biomechanical problems in the application of zirconium dioxide and pure titanium abutment in the anterior teeth area. A kind of aesthetic abutment material conforming to biomechanics needs to be studied urgently.
OBJECTIVE: To compare the adhesion of Porphyromonas gingivalis and whole saliva on the surface of polyetheretherketone (PEEK), zirconium dioxide (ZrO2), and pure titanium (Ti). 
METHODS: Specimens with the shape of a disk were prepared from PEEK, ZrO2, and Ti. The surface morphology feature was observed by scanning electron microscope. Surface roughness was determined by atomic force microscope. Contact angle was measured using contact angle measuring instrument. The adhesion force of Porphyromonas gingivalis on the surface of materials was measured using an atomic force microscope probe. The initial bacterial adhesion on materials in the suspension of Porphyromonas gingivalis and whole saliva was evaluated by scanning electron microscope and Cell Counting Kit-8 assay. 
RESULTS AND CONCLUSION: (1) Scanning electron microscope showed that the surface of the PEEK and Ti specimen was grooved, and the groove of PEEK was more than that of Ti, and there were a few microholes on the surface of ZrO2. Atomic force microscopy showed that parallel groove undulations were observed on the surfaces of the three specimens. The groove peak and valley structure of PEEK and ZrO2 was more prominent than that of Ti, and the porous concave and convex structure of the ZrO2 surface was more than that of PEEK. (2) The surface roughness of ZrO2 was greater than that of Ti (P < 0.05). The pairwise comparison between other groups showed no significant difference (P > 0.05). There was no significant difference between the contact angles of the three groups (P > 0.05). (3) A significantly lower adhesion force was identified for PEEK than for ZrO2 and Ti (P < 0.05). (4) The results of scanning electron microscope observation showed that the bacterial adhesion on the PEEK surface was the least, while that on the Ti surface was the most. The Cell Counting Kit-8 assay result showed that the initial bacterial adhesion on the surface of PEEK was significantly lower than that on the surface of ZrO2 and Ti (P < 0.05). (5) The results suggest that PEEK has more advantages to inhibit initial bacterial adhesion than conventional abutment materials such as ZrO2 and Ti.  

Key words: polyetheretherketone, zirconium dioxide, pure titanium, bacterial adhesion, implant denture, abutment material

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