中国组织工程研究 ›› 2021, Vol. 25 ›› Issue (14): 2211-2218.doi: 10.3969/j.issn.2095-4344.3146

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

运动后针刺腓肠肌干预肥胖大鼠肌腱退行性病变

刘云逸,王  博,王  琳   

  1. 北京体育大学运动医学与康复学院,北京市   100084
  • 收稿日期:2019-11-08 修回日期:2019-11-18 接受日期:2020-05-22 出版日期:2021-05-18 发布日期:2020-12-30
  • 通讯作者: 王博,博士,讲师,北京体育大学运动医学与康复学院,北京市 100084
  • 作者简介:刘云逸,男,1994年生,湖北省黄冈市人,汉族,2019年北京体育大学毕业,硕士,主要从事运动损伤研究。

Effects of post-exercise gastrocnemius needling on Achilles tendon degeneration in obese rats

Liu Yunyi, Wang Bo, Wang Lin   

  1. School of Sports Medicine and Rehabilitation, Beijing Sport University, Beijing 100084, China
  • Received:2019-11-08 Revised:2019-11-18 Accepted:2020-05-22 Online:2021-05-18 Published:2020-12-30
  • Contact: Wang Bo, MD, Lecturer, School of Sports Medicine and Rehabilitation, Beijing Sport University, Beijing 100084, China
  • About author:Liu Yunyi, Master, School of Sports Medicine and Rehabilitation, Beijing Sport University, Beijing 100084, China

摘要:

文题释义:
跟腱退行性病变:跟腱炎和跟腱末端病的早期表现,主要的组织学表现有肌腱胶原纤维比例和相关蛋白酶生长因子表达出现明显变化。
力学生长因子(MGF):这类生长因子的表达受力学变化的影响很大,组织受力增加,其表达出现明显升高。典型的力学生长因子有转化生长因子β1和胰岛素样生长因子1等。

背景:运动后腓肠肌张力升高是导致退行性病变的主要原因,超重者的发病风险更高,通过针刺腓肠肌可以降低运动后腓肠肌张力。
目的:观察通过针刺运动后力学生长因子和组织蛋白表达变化,从而探讨针刺腓肠肌对跟腱退行性病变风险的影响。
方法:32只12周龄雄性SD大鼠给予高脂饲料喂养4周建立肥胖大鼠模型,体质量(500.27±29.11) g,随机分为4组:空白对照组、运动组、针刺组及运动针刺组。运动强度为60 min 60%的最大摄氧量(15.6 m/min)以-16°进行离心运动,每周运动6 d持续4周;每次运动结束后分别对针刺组和运动针刺组进行针刺干预,针刺位置为腓肠肌中部,每次针刺时间为5 min。第5周对大鼠跟腱及腓肠肌进行取材,苏木精-伊红染色观察跟腱组织结构变化;免疫荧光染色检测跟腱内胶原蛋白以及相关细胞因子表达;Western-blot分析腓肠肌转化生长因子β1表达。实验方案已经获得北京体育大学实验动物伦理委员会批准。
结果与结论:①运动与运动针刺组大鼠跟腱组织出现纤维排列紊乱的现象;②运动组大鼠腓肠肌中转化生长因子β1蛋白表达显著高于其他3组(P < 0.05),运动针刺组显著高于对照和针刺组(P < 0.05);③运动组大鼠跟腱中Ⅰ型胶原蛋白水平显著低于其他3组(P < 0.05);Ⅲ型胶原蛋白水平显著高于其他3组(P < 0.05);运动针刺组Ⅲ型胶原蛋白水平高于针刺和对照组(P < 0.05);④运动组大鼠跟腱中基质金属蛋白酶1表达显著低于其他3组(P < 0.05);基质金属蛋白酶13表达显著高于其他3组(P < 0.05);⑤运动组大鼠跟腱中转化生长因子β1表达显著高于其他3组(P < 0.05),运动针刺组显著高于针刺和对照组(P < 0.05);⑥胰岛素样生长因子1表达各组间比较没有显著差异;⑦结果说明,运动后针刺腓肠肌可以降低肥胖大鼠跟腱退行性病变风险。

https://orcid.org/0000-0002-2369-1129 (刘云逸) 

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程


关键词: 针刺, 腓肠肌, 跟腱退行性病变, 力学生长因子, 肌张力, 胶原蛋白, 基质金属蛋白酶

Abstract: BACKGROUND: Post-exercise gastrocnemius tension increase is the main cause of degeneration, and overweight people are more susceptible. The gastrocnemius needling treatment has been considered as an effective way to reduce post-exercise muscle tension. 
OBJECTIVE: To study the effect of post-exercise gastrocnemius needling treatment on the risk of Achilles tendon degeneration by observing the changes in the expression of mechanical growth factors and tissue proteins after needling.
METHODS: Thirty-two 12-week-old male Sprague-Dawley rats were fed with high fat diet for 4 weeks to establish obese rats with a body weight of (500.27±29.11) g. All rat models were randomly divided into blank control group, exercise group, needling group and post-exercise needling group. Eccentric running at -16° was performed, and the exercise intensity was set at 60% of the maximum oxygen uptake (15.6 m/min) for 60 minutes. The exercise was performed 6 days per week for 4 continuous weeks. Needling treatment was performed in the needling group and the post-exercise needling group at the end of each exercise. The needle position was in the middle of the gastrocnemius muscle, and the needling time was 5 minutes. Achilles tendon and gastrocnemius of rats were harvested followed by histological analysis in the 5th week. Hematoxylin-eosin staining was used to observe the structural changes of Achilles tendon; immunofluorescence staining was used to detect the expression of collagen and related cytokines in the Achilles tendon; western blot assay was used to detect transforming growth factor β1 expression in the gastrocnemius. The study protocol was approved by the Experimental Animal Ethics Committee of Beijing Sport University.
RESULTS AND CONCLUSION: The collagen fibers of the Achilles tendon were disordered in the exercise and post-exercise needling groups. The expression of transforming growth factor β1 in the gastrocnemius was significantly higher in the exercise group than the other three groups (P < 0.05), and was significantly higher in the post-exercise needling group than the blank control and needling groups (P < 0.05). The expression of type I collagen in the Achilles tendon was significantly lower in the exercise group than in the other three groups (P < 0.05). The expression of type III collagen was significantly higher in the exercise group than in the other three groups (P < 0.05), and significantly higher in the post-exercise needling group than the needling and blank control groups (P < 0.05). The expression of matrix metalloproteinase 1 in the Achilles tendon was significantly lower in the exercise group than the other three groups (P < 0.05), whereas the expression of matrix metalloproteinase 13 in the Achilles tendon was significantly higher in the exercise group than the other three groups (P < 0.05). The expression of transforming growth factor β1 in the Achilles tendon was significantly higher in the exercise group than the other three groups (P < 0.05), and was significantly higher in the post-exercise needling group than the needling and control groups (P < 0.05). There was no significant difference in insulin-like growth factor 1 expression among the four groups. To conclude, post-exercise gastrocnemius needling can reduce the risk of Achilles tendon degeneration in obese rats.


Key words: needling, gastrocnemius, Achilles tendon degeneration, mechanical growth factor, tension, collagen, matrix metalloproteinase

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