中国组织工程研究

中国组织工程研究 ›› 2020, Vol. 24 ›› Issue (27): 4356-4363.doi: 10.3969/j.issn.2095-4344.2717

• 骨与关节综述 bone and joint review • 上一篇    下一篇

基因治疗骨关节炎的概念、机制及问题

胡中岭1，李彬彬1，崔逸爽2，王  茜2，张  辉3，李琪佳2，王志强1   

  1. 1华北理工大学附属医院骨科，河北省唐山市  063000；2华北理工大学医学实验研究中心，河北省唐山市  063000；3唐山市第二医院关节一科，河北省唐山市  063000
  • 收稿日期:2019-11-16 修回日期:2019-11-20 接受日期:2020-01-02 出版日期:2020-09-28 发布日期:2020-09-09
  • 通讯作者: 王志强，主任医师，教授，博士生导师，华北理工大学附属医院骨科，河北省唐山市 063000
  • 作者简介:胡中岭，男，1990年生，河北省保定市人，满族，华北理工大学在读硕士，主要从事骨和软骨组织工程研究。
  • 基金资助:
    国家科技支撑计划课题资助项目(2012BAE06B03)；河北省科技支撑资助项目(16277776D)；河北省医学科学研究重点课题计划资助项目(20160225)

Concept, mechanism and problem of gene therapy for osteoarthritis

Hu Zhongling1, Li Binbin1, Cui Yishuang2, Wang Qian2, Zhang Hui3, Li Qijia2, Wang Zhiqiang1   

  1. 1Department of Orthopedics, Affiliated Hospital, North China University of Science and Technology, Tangshan 063000, Hebei Province, China; 2Medical Experimental Research Center of North China University of Science and Technology, Tangshan 063000, Hebei Province, China; 3First Department of Arthrology, Tangshan Second Hospital, Tangshan 063000, Hebei Province, China
  • Received:2019-11-16 Revised:2019-11-20 Accepted:2020-01-02 Online:2020-09-28 Published:2020-09-09
  • Contact: Wang Zhiqiang, Chief physician, Professor, Doctoral supervisor, Department of Orthopedics, Affiliated Hospital, North China University of Science and Technology, Tangshan 063000, Hebei Province, China
  • About author:Hu Zhongling, Master candidate, Department of Orthopedics, Affiliated Hospital, North China University of Science and Technology, Tangshan 063000, Hebei Province, China
  • Supported by:
    the National Science and Technology Support Program, No. 2012BAE06B03; the Hebei Science and Technology Support Project, No. 16277776D; the Key Support Project of Hebei Province Medical Science Research, No. 20160225

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摘要：

文题释义：

基因治疗：基因疗法是指使用病毒或非病毒载体以直接转导或离体细胞介导的方法把核酸运送到靶组织，并通过在软骨损伤部位表达治疗性转基因产物来促进软骨修复。

miRNA技术：通过增强降解、抑制转录后翻译等机制来修饰相关基因的转录后调节，从而达到调节软骨发育的体内平衡。

背景：特定基因在特定的损伤部位的表达可增强细胞再生。

目的：总结基因治疗的概念，对基因治疗的策略和基因治疗骨关节炎的机制进行综述，介绍基因治疗的研究方向。

方法：以“Gene therapy、Cartilage、Cartilage repair、Osteoarthritis、Expression vectors、miRNA”等关键词，广泛查阅PubMed、Science Direct等外文数据库中关于基因治疗骨关节炎及其相关内容的文献，并进行分析和总结。

结果与结论：基因治疗骨关节炎可以应用到骨关节炎发病的各个环节，并对相关物质代谢产生影响，通过病毒或非病毒载体，把目的基因递送到靶细胞。目前基因治疗的重点在加快细胞修复和减少炎症反应方面，同时基因载体和目的基因的多种选择提供了更好的个体化治疗方案。临床上基因治疗骨关节炎的具体机制尚不明确，存在基因突变的可能性，需深入研究验证安全性。

ORCID: 0000-0002-5491-9405(胡中岭)

中国组织工程研究杂志出版内容重点：人工关节；骨植入物；脊柱；骨折；内固定；数字化骨科；组织工程

关键词: 基因治疗, 骨关节炎, miRNA, 生长因子, 病毒载体

Abstract:

BACKGROUND: Specific gene expression at specific injury sites enhances cell regeneration.

OBJECTIVE: To summarize the concept of gene therapy, to review the strategies of gene therapy and the mechanism of gene therapy for osteoarthritis, and to introduce the research direction of gene therapy.

METHODS: Extensive consulted the literature on osteoarthritis gene therapy and related content in foreign databases such as PubMed and Science Direct. The key words were “Gene therapy, Cartilage, Cartilage repair, Osteoarthritis, Expression vectors, miRNA”. Above data were analyzed and summarized.

RESULTS AND CONCLUSION: Gene therapy for osteoarthritis can be applied to all aspects of the pathogenesis of osteoarthritis and affect the metabolism of related substances. The target gene is delivered to



the target cells through viral or non-viral vectors. At present, the focus of gene therapy is to accelerate cell repair and reduce inflammatory response. At the same time, multiple choices of gene vectors and target genes provide better individualized treatment options. The specific mechanism of gene therapy for osteoarthritis in clinic is not clear, and there is the possibility of gene mutation, which needs to be further studied to verify the safety.

Key words: gene therapy, osteoarthritis, miRNA, growth factor, viral vector

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