中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (33): 6211-6214.doi: 10.3969/j.issn.2095-4344.2012.33.026

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

一定条件下电压信号与血清蛋白浓度的关系

薛忠晋1，周汉昌1，张学俊2，高志强1，冯巧玲1   

  1. 1中北大学仪器科学与动态测试教育部重点实验室，山西省太原市 030051；
    2中北大学理学院，山西省太原市 030051  
  • 收稿日期:2011-12-26 修回日期:2012-02-17 出版日期:2012-08-12 发布日期:2013-07-15
  • 通讯作者: 高志强，在读博士研究生，中北大学仪器科学与动态测试教育部重点实验室，山西省太原市 030051 gaozq418@126.com
  • 作者简介:薛忠晋☆，男，1970年生，山西省大同市人，中北大学在读博士，工程师，经济师，主要从事节能减排、综合管理及技术方面的研究工作。

Voltage signal and serum protein concentration under certain conditions

Xue Zhong-jin1, Zhou Han-chang1, Zhang Xue-jun2, Gao Zhi-qiang1, Feng Qiao-ling1   

  1. 1Key Laboratory of Instrumentation Science & Dynamic Measurement of Ministry of Education, North University of China, Taiyuan 030051, Shanxi Province, China;
    2Faculty of Science, North University of China, Taiyuan 030051, Shanxi Province, China
  • Received:2011-12-26 Revised:2012-02-17 Online:2012-08-12 Published:2013-07-15
  • Contact: Gao Zhi-qiang, Studying for master’s degree, Key Laboratory of Instrumentation Science & Dynamic Measurement of Ministry of Education, North University of China, Taiyuan 030051, Shanxi Province, China gaozq418@126.com
  • About author:Xue Zhong-jin☆, Studying for doctorate, Engineer, Economist, Key Laboratory of Instrumentation Science & Dynamic Measurement of Ministry of Education, North University of China, Taiyuan 030051, Shanxi Province, China

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摘要：

背景：蛋白质的定量测定在各个领域中有着重要的意义。
目的：探讨血清蛋白浓度与共振散射光强度的关系。
方法：以405 nm的激发光波照射在四羧基酞菁锌与血清蛋白混合溶液上，共振散射光在475 nm处强度最强，Si-PIN光电探测器把475 nm的出射光信号转换成电压信号。
结果与结论：实验结果表明，2.0×10-4 mol/L四羧基酞菁锌与血清蛋白混合溶液的实验数据在10~50 g/L范围内线性关系良好，相关系数r=0.993 9，结果满意。

关键词: 共振瑞利散射, 四羧基酞菁锌, 血清蛋白, 浓度, 电压信号

Abstract:

BACKGROUND: The quantitative determination of protein in various fields has important significance.
OBJECTIVE: To study the relationship between serum protein concentration and resonance scattering light intensity
METHODS: Excitation light irradiating of 405 nm was used on the mixture of Zn(II) tetracarboxy phthalocyanine and serum proteins solution. The resonance scattering light intensity was the strongest at 475 nm. The Si-PIN photodetector transformed the 475 nm light signals into voltage signals in order to study the relationship between voltage signal and protein concentration under certain conditions.
RESULTS AND CONCLUSION: The experimental data about the mixture of Zn(II) tetracarboxy phthalocyanine in 2.0×10-4 mol/L and serum proteins solution had good linear relationship within the range from 10 to 50 g/L and the correlation coefficient r=0.993 9. The results were satisfactory.

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