中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (17): 2678-2683.doi: 10.3969/j.issn.2095-4344.1719

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

富血小板血浆复合骨骼肌干细胞可促进骨修复

殷 诺,薛 锋,肖海军,丁 亮,袁君杰,潘明芒,喻 都,鞠金勇,朱龙章   

  1. 上海市奉贤区中心医院(上海交通大学附属第六人民医院南院),上海市 201499
  • 修回日期:2019-02-17 出版日期:2019-06-18 发布日期:2019-06-18
  • 通讯作者: 朱龙章,硕士,副主任医师,上海市奉贤区中心医院(上海交通大学附属第六人民医院南院),上海市 201499
  • 作者简介:殷诺,男,1982年生,黑龙江省哈尔滨市人,汉族,2008年上海交通大学医学院毕业,硕士,主治医师,主要从事组织工程骨修复的相关研究。
  • 基金资助:

    上海市医学重点专科建设项目(ZK2015B06),项目负责人:薛锋;上海市奉贤区科委项目(奉卫科2010-01),项目负责人:殷诺;上海市健康医学院种子基金项目(HMSF-17-21-025),项目负责人:殷诺

Bone repair using skeletal muscle stem cells combined with platelet-rich plasma

Yin Nuo, Xue Feng, Xiao Haijun, Ding Liang, Yuan Junjie, Pan Mingmang, Yu Du, Ju Jinyong, Zhu Longzhang   

  1. Shanghai Fengxian District Central Hospital (South Campus, Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University), Shanghai 201499, China
  • Revised:2019-02-17 Online:2019-06-18 Published:2019-06-18
  • Contact: Zhu Longzhang, Master, Associate chief physician, Shanghai Fengxian District Central Hospital (South Campus, Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University), Shanghai 201499, China
  • About author:Yin Nuo, Master, Attending physician, Shanghai Fengxian District Central Hospital (South Campus, Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University), Shanghai 201499, China
  • Supported by:

    Shanghai Key Medical Specialist Construction Project, No. ZK2015B06 (to XF); a grant from Shanghai Fengxian District Science and Technology Commission, Fengweike2010-01 (to YN); and the Seed Fund of Shanghai University of Medicine & Health Sciences, No. HMSF-17-21-025 (to YN)

摘要:

文章快速阅读:

文题释义:
富血小板血浆:
1993年Hood等首先提出富血小板血浆概念,富血小板血浆是自体全血经离心后得到的血小板浓缩物,并发现富血小板血浆含有丰富的血小板,其数目比全血中数目高3倍以上。血小板中含有大量的生长因子,如血小板衍生生长因子、转化生长因子β、类胰岛素生长因子、表皮生长因子、血管内皮生长因子等。目前,在口腔科及骨科领域,富血小板血浆研究很热门。
骨骼肌干细胞:是来源于骨骼肌的间充质干细胞,又称肌源性间充质干细胞。间充质干细胞是干细胞家族的重要成员,来源于发育早期的中胚层,属于多能干细胞,间充质干细胞最初在骨髓中发现,因其具有多向分化潜能、造血支持和促进干细胞植入、免疫调控和自我复制等特点而日益受到人们的关注。

 

摘要
背景:
富血小板血浆含有的血小板颗粒富含多种活性因子,将其作为促进干细胞分化的细胞因子进行组织工程骨修复具有一定的优势。
目的:探讨富血小板血浆复合骨骼肌干细胞促进兔肱骨近端骨缺损修复的效果。
方法:制备兔富血小板血浆,提取兔比目鱼肌组织中的骨骼肌干细胞。将28只新西兰白兔随机分为4组,自然愈合组、骨骼肌干细胞组,富血小板血浆组、复合材料组,每组7只,均制备肱骨近端骨缺损模型,后3组造模后骨缺损处分别植入同种异体骨骼肌干细胞、自体富血小板血浆、富血小板血浆复合骨骼肌干细胞。术后进行CT检查及组织学观察骨愈合情况。
结果与结论:①术后4周起复合材料组影像学上成骨速率明显高于其他3组(P < 0.05);骨骼肌干细胞组、富血小板血浆组成骨速率无明显差异(P > 0.05),均明显高于自然愈合组(P < 0.05);②复合材料组骨缺损处组织学评分最高,骨骼肌干细胞组和富血小板血浆组组织学评分无明显差异,自然愈合组组织学评分最低;③结果表明,富血小板血浆和骨骼肌干细胞都具有促进骨修复的作用,而二者结合能更好地修复骨缺损。


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
ORCID: 0000-0001-9736-3382(殷诺)

关键词: 富血小板血浆, 骨骼肌干细胞, 肱骨近端骨缺损, 诱导成骨, 骨修复, 骨折愈合, 成骨速度

Abstract:

BACKGROUND: Platelet-rich plasma contains a variety of active factors, which has certain advantages as a cytokine that promotes stem cell differentiation for bone tissue engineering.
OBJECTIVE: To investigate the effect of skeletal muscle stem cells combined with platelet-rich plasma to promote the repair of rabbit bone.
METHODS: (1) Rabbit platelet-rich plasma was prepared and skeletal muscle stem cells from rabbit soleus muscle were isolated. Twenty-eight New Zealand white rabbits were randomly divided into four groups (n=7/group), in which the proximal humeral bone defect model was prepared in each rabbit, followed by implantation of nothing (natural healing group), allogeneic skeletal muscle stem cells, autologous platelet-rich plasma or platelet-rich plasma combined with skeletal muscle stem cells, respectively. CT and histological examinations were performed to detect bone healing postoperatively.
RESULTS AND CONCLUSION: In the postoperative 4-week CT film, new bone formation was faster in the combination group than the other three groups (P < 0.05). No significant difference in the bone formation rate was observed in the skeletal muscle stem cell and platelet-rich plasma groups (P > 0.05), but the bone formation rate in these two groups was significantly better than that in the natural healing group (P < 0.05). (2) Histological score of the one defect specimens was highest in the combination group and lowest in the natural healing group, and there was no significant difference between the skeletal muscle stem cell and platelet-rich plasma groups. These results reveal that both platelet-rich plasma and skeletal muscle stem cells could promote bone repair, and their combination could achieve better outcomes in bone repair.

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