中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (3): 470-475.doi: 10.3969/j.issn.2095-4344.0592

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

补肾健脾方影响高脂肪饮食骨质疏松模型大鼠脂代谢及瘦素的变化

李  平1，林  煜1，朱  曦2，张怡元1，肖莉莉1，华  鹏1，林学义1   

  1.  (1厦门大学附属福州第二医院骨科，福建省福州市  350007；2福建中医药大学，福建省福州市  350102)
  • 收稿日期:2018-07-23
  • 通讯作者: 林煜，博士，讲师，厦门大学附属福州第二医院骨科，福建省福州市 350007
  • 作者简介:李平，男，1974年生，福建省福州市人，汉族，1997年福建中医药大学毕业，副主任医师，主要从事中医骨伤临床与基础研究。
  • 基金资助:

    福建省自然科学基金(2017J01332)，项目负责人：林煜；福州市卫生系统科技项目(2017-S-wq15)，项目负责人：李平

Bushen Jianpi Recipe affects lipid metabolism and leptin expression in rats with osteoporosis receiving a high fat diet

Li Ping1, Lin Yu1, Zhu Xi2, Zhang Yiyuan1, Xiao Lili1, Hua Peng1, Lin Xueyi1   

  1.  (1Department of Orthopedics, Fuzhou Second Hospital Affiliated to Xiamen University, Fuzhou 350007, Fujian Province, China; 2Fujian University of Traditional Chinese Medicine, Fuzhou 350102, Fujian Province, China)
  • Received:2018-07-23
  • Contact: Lin Yu, MD, Lecturer, Fujian University of Traditional Chinese Medicine, Fuzhou 350102, Fujian Province, China
  • About author:Li Ping, Associate chief physician, Department of Orthopedics, Fuzhou Second Hospital Affiliated to Xiamen University, Fuzhou 850007, Fujian Province, China
  • Supported by:

    the Natural Science Foundation of Fujian Province, No. 2017J01332 (to LY); the Science and Technology Project of Fuzhou Health Department, No. 2017-S-wq15 (to LP)

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摘要：

文章快速阅读：

文题释义：
补肾健脾方：“肾主骨”、“补先后天”理论为组方原则，是针对“肾亏脾虚”的病机特点，以煅狗骨、淫羊藿、山茱萸、党参、山药组方而成，通过补肾健脾，达到强筋壮骨之目的方剂。
瘦素脂代谢与骨质疏松：老年女性，由于绝经也可导致体内瘦组织与脂肪的成分的改变，而且现阶段发现骨质疏松在肥胖的老年人中的发生率要大于非肥胖者，由此推断瘦素可能是骨质疏松症发病机制中的一个重要调节因素。
摘要
背景：绝经也可导致体内瘦组织与脂肪成分的改变，现阶段发现骨质疏松在肥胖的老年人中的发生率要大于非肥胖者，由此推断瘦素可能是骨质疏松症发病机制中的一个重要调节因素。
目的：探讨补肾健脾方对高脂肪饮食骨质疏松大鼠脂代谢及瘦素的影响。
方法：将6月龄SPF级雌性SD大鼠220只随机分成模型组170只和假手术组50只。模型组行双侧卵巢结扎切除术，假手术组除未行卵巢结扎切除外，其余步骤同模型组。手术后12周，模型鉴定成功后，手术组随机分成正常饮食模型组、高脂饮食模型组、高脂饮食健骨颗粒组和高脂饮食辛伐他丁组各40只。各组于术后第13周开始给药，健骨颗粒组给予健骨颗粒2 g/(kg•d)，生理盐水2 mL溶解后灌胃，辛伐他丁组给予辛伐他丁100 μg/(kg•d)灌胃，假手术组和正常饮食模型组以2 mL生理盐水灌胃，1次/d。于用药2，6，12，24周后测体质量，取血清、腰椎和股骨近端备用。双能X射线测定右侧胫骨骨密度，ELISA法测定血清骨钙素、血清抗酒石酸酸性磷酸酶5b(TRACT-5b)、总胆固醇、三酰甘油、瘦素、瘦素受体的水平，qPCR法测定大鼠骨组织瘦素、瘦素受体mRNA表达。
结果与结论：①高脂饮食健骨颗粒组大鼠的体质量高于同期正常饮食模型组和高脂饮食模型组，但低于假手术组和高脂饮食辛伐他汀组，同时表达的瘦素、瘦素受体、骨钙素水平均高于正常饮食模型组和高脂饮食模型组，TRACT-5b、胆固醇、三酰甘油的浓度均低于正常饮食模型组和高脂饮食模型组，各组比较差异均有显著性意义(P < 0.05)；②结果表明，补肾健脾方可以调整大鼠脂代谢紊乱、降低血液黏滞度、改善血管壁结构的同时还能通过上调大鼠血清和骨组织中瘦素、瘦素受体的表达，改善大鼠骨代谢水平，防治原发性骨质疏松症。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0003-4680-2669(李平)

关键词: 补肾健脾, 健骨颗粒, 骨质疏松, 脂代谢, 瘦素, 组织构建

Abstract:

BACKGROUND: Menopause can also lead to changes in lean tissue and fat composition. Osteoporosis is found to be more common in obese elderly than in non-obese people. Thereafter, leptin may be an important regulatory factor in the pathogenesis of osteoporosis.
OBJECTIVE: To investigate the effect of Bushen Jianpi Recipe on lipid metabolism and leptin in high fat diet rats with osteoporosis.
METHODS: Totally 220 SPF Sprague-Dawley rats aged 6 months were randomly divided into model group (n=170) and sham group (n=50). The model group underwent bilateral ovarian ligation and the sham group received the same intervention as the model group except that ovarian ligation was not performed. At 12 weeks after surgery, the rat models were randomized into normal, high-fat, high-fat plus Jiangu Granule (2 g/(kg•d)), and high-fat plus simvastatin (100 μg/(kg•d)) groups (n=40 per group). The administration began at 13 weeks after surgery via gavage. The normal and sham groups were given 2 mL of normal saline, once daily. After 2, 6, 12 and 24 weeks of treatment, the body mass was measured. The serum, lumbar vertebrae and proximal femurs were removed. The bone mineral density of the right tibia was measured by double energy X-ray. The concentration of serum osteocalcin, tartrate-resistant acid phosphatase isoform 5b (TRACP-5b), total cholesterol, triacylglycerol, leptin and leptin receptor were detected by ELISA. The mRNA expression of leptin and leptin receptor in bones was determined by qPCR.
RESULTS AND CONCLUSION: The rat body mass in the high-fat plus Jiangu Granule group was higher than that in the normal and high-fat groups at the same time, but lower than that in the sham and high-fat plus simvastatin groups. The expression levels of leptin and leptin receptor, and osteocalcin level in the high-fat plus Jiangu Granule group were significantly higher than those in the normal and high-fat groups, and the contents of TRACP-5b, cholesterol, and triacylglycerol were significantly lower than those in the Leptin and leptin receptor, the BGP concentration were higher than those in the normal diet model group and the high fat diet model group. The concentrations of TRACT-5b, cholesterol, and triglycerides were lower than those in the normal and high-fat groups (P < 0.05). These results indicate that Bushen Jianpi Recipe can regulate lipid metabolism disturbance, reduce blood viscosity and improve the structure of blood vessel wall. It can also increase the expression of leptin and leptin receptor in serum and bones of rats, improve bone metabolism, and prevent and treat primary osteoporosis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Drugs, Chinese Herbal, Osteoporosis, Leptin, Receptors, Leptin, Tissue Engineering

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