中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (26): 4101-4109.doi: 10.3969/j.issn.2095-4344.0932

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

磷酸镁/胶原肽复合骨水泥：胶原肽的缓凝作用

曹霄峰1，2，王毅虎1，2，3，路浩军1，2，马 铭1，2，郭燕川1，2，3，毛克亚4，李江涛5   

  1. 中国科学院理化技术研究所，1光化学转换与功能材料重点实验室，5低温工程学重点实验室，北京市 100190；2中国科学院理化技术研究所杭州研究院生物材料研究中心，浙江省杭州市310018；3中国科学院大学，北京市 100049；4解放军总医院(301医院)骨科，北京市 100853
  • 收稿日期:2018-01-16
  • 通讯作者: 郭燕川，博士，研究员，博士生导师，中国科学院理化技术研究所，北京市 100190
  • 作者简介:曹霄峰，男，1982年生，安徽省怀远县人，2011年南京大学毕业，博士，助理研究员，主要从事骨修复材料的研究。
  • 基金资助:

    国家自然科学基金项目(51372276，21506236)；中国科学院科技服务网络计划(STS计划) (KFJ-STS-ZDTP-016)

Magnesium phosphate/collagen peptide composite cements: the setting retardation effect of collagen peptides

Cao Xiao-feng1, 2, Wang Yi-hu1, 2, 3, Lu Hao-jun1, 2, Ma Ming1, 2, Guo Yan-chuan1, 2, 3, Mao Ke-ya4, Li Jiang-tao5   

  1. 1Key Laboratory of Photochemical Conversion and Optoelectronic Material, 5Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China; 2Biomaterials Research Center, Hangzhou Branch of Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Hangzhou 310018, Zhejiang Province, China; 3University of Chinese Academy of Sciences, Beijing 100049, China; 4Department of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, China
  • Received:2018-01-16
  • Contact: Guo Yan-chuan, Ph.D., Professor, Doctoral supervisor, Key Laboratory of Photochemical Conversion and Optoelectronic Material, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
  • About author:Cao Xiao-feng, Ph.D., Assistant professor
  • Supported by:

    the National Natural Science Foundation of China, No. 51372276, 21506236; Science and Technology Service Network Initiative of the Chinese Academy of Sciences, No. KFJ-STS-ZDTP-016

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摘要：

文章快速阅读：

 
文题释义：
磷酸镁骨水泥：磷酸镁骨水泥粉末由氧化镁、磷酸盐、缓凝剂等成分组成，与固化液混合后具有自固化性质。磷酸镁骨水泥具有优异的生物相容性和生物活性，与骨骼具有良好的粘结作用，易被再吸收，是一种较为理想的骨缺损修复材料。但磷酸镁骨水泥也存在反应速率快，凝固时间短，反应放热高等缺点，所以需要对磷酸镁骨水泥的配方进行优化。

胶原肽：主要是从动物的骨骼和皮肤中提取，是胶原的水解产物。相比于胶原，胶原肽更易被人体吸收和消化，具有预防和治疗骨关节炎以及骨质疏松的功效。最新的研究结果表明骨胶原肽对成骨细胞的增殖与分化具有促进作用。

 

背景：磷酸镁骨水泥是一种具有良好生物相容性和生物活性的骨修复材料，克服了磷酸钙骨水泥的一些缺点，但也存在固化过程反应速率快，凝固时间短，高放热等缺点。胶原肽是一种多肽混合物，具有增强人体骨密度，预防和治疗骨关节炎以及骨质疏松等性质。目前文献中未见将胶原肽引入磷酸镁骨水泥制成磷酸镁/胶原肽复合骨水泥并研究其理化性质和细胞毒性的报道。
目的：制备一种固化速率与反应放热可调的磷酸镁/胶原肽复合骨水泥，研究其理化性质与细胞毒性。
方法：将低活性氧化镁、磷酸二氢钾、十水合四硼酸钠混合制成磷酸镁骨水泥，将不同量的胶原肽引入其中，形成磷酸镁/胶原肽复合骨水泥。测试了复合骨水泥的初凝、终凝时间，反应过程温度变化以及模拟体液中浸泡1 d和7 d后的抗压强度。四唑盐比色实验测试了骨水泥的细胞毒性。
结果与结论：将胶原肽加入磷酸镁骨水泥中制得了磷酸镁/胶原肽复合骨水泥材料。胶原肽的含量影响了复合骨水泥的理化和生物性质。胶原肽含量的增加延长了复合骨水泥材料的初凝和终凝时间，降低了复合骨水泥水化固化过程中的最高温度，延后了出现最高温度的时间。磷酸镁骨水泥以及复合骨水泥的水化固化产物都是MgKPO4•6H2O。上述实验结果表明胶原肽对复合骨水泥具有良好的缓凝作用，不改变固化产物的物相。机械性质的实验结果表明适量的胶原肽能够提高复合骨水泥的抗压强度，但进一步提高胶原肽的含量会降低复合骨水泥的抗压强度。同时试样在模拟体液中静置时间也对骨水泥的抗压强度具有明显的影响。四唑盐比色实验结果表明胶原肽的加入进一步促进了MC3T3-E1细胞和L929细胞的增殖。结果为推动磷酸镁/胶原肽复合骨水泥在临床中应用提供了技术依据。

 

ORCID: 0000-0003-4435-5869(曹霄峰)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 骨水泥, 镁化合物, 鸟粪石, 多肽, 胶原肽, 缓凝作用, 抗压强度, 细胞毒性, 生物材料

Abstract:

BACKGROUND: Magnesium phosphate cement (MPC) is a kind of bone repair material with excellent biocompatibility and bioactivity, which has overcome some disadvantages of calcium phosphate cements. However, the MPC still has some drawbacks such as the fast reaction rate in the setting process, the short initial and final setting times, and the high temperature in the setting process. Collagen peptides (CP) as polypeptide mixtures have the properties of enhancing bone mineral density, and preventing and treating osteoarthritis and osteoporosis. So far, there are few reports on the composite cements composed of MPC and CP. The physicochemical properties and cytotoxicity of the composites cements have not been well documented.

OBJECTIVE: To prepare the MPC/CP composite cement with controllable setting rate and setting reaction heat and to study the physicochemical properties and cytotoxicity of the composite cement.
METHODS: The MPC were prepared by combining low-activity MgO, KH2PO4, and Na2B4O7•10H2O. Different amounts of CP were introduced into the MPC to form MPC/CP composite cements. We measured the initial and final setting times, the temperature evolution during the setting process and the compressive strength of the composite cements soaked in the simulated body fluid for 1 and 7 days. The MTT assay was used to evaluate the cytotoxicity of the composite cements.

RESULTS AND CONCLUSION: The addition of CP altered the physicochemical and biological properties of the composite bone cements. With the increasing of the CP content, prolonged initial and final setting times, reduced maximum temperatures during the setting process, and delayed time for the maximum temperature were observed. The hydrated and cured products of MPC and the composite bone cement were MgKPO4•6H2O. The above experimental results suggested that the CP had a good retarding effect on the composite bone cement without changing the phase of the cured product. Experimental findings regarding the mechanical properties showed that with the increasing of the CP content, the compressive strengths of the composite cements increased firstly and then decreased. The storing time in the simulated body fluid was also the influencing factor for the compressive strength of the composite cement. Results from the MTT assay showed that the addition of CP promoted the proliferation of MC3T3-E1 cells and L929 cells. Overall findings provide a technical basis for the clinical application of MPC/CP composite cements.

 

Key words: Magnesium Compounds, Biocompatible Materials, Tissue Engineering

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