中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (52): 7757-7764.doi: 10.3969/j.issn.2095-4344.2016.52.001

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

可注射聚富马酸丙二醇酯/β-磷酸三钙骨水泥的体外生物活性及其降解性

马正宇2，杨  峰2，王  靖2，刘昌胜1，2
  

  1. 1华东理工大学超细材料制备与应用教育部重点实验室，上海市  200237；2华东理工大学教育部医用生物材料工程研究中心，上海市  200237
  • 收稿日期:2016-09-22 出版日期:2016-12-16 发布日期:2016-12-16
  • 通讯作者: 王靖，副教授，硕士生导师，华东理工大学教育部医用生物材料工程研究中心，上海市 200237 刘昌胜，教育部“长江学者”特聘教授，博士生导师，华东理工大学教育部医用生物材料工程研究中心，上海市 200237
  • 作者简介:马正宇，女，1990年生，河南省安阳市人，汉族，硕士，主要从事生物医用材料研究。
  • 基金资助:

    国家自然科学基金(31330028，31470923，31271011)

In vitro bioactivity and degradability of injectable poly(propylene fumarate)/ beta-tricalcium phosphate bone cement

Ma Zheng-yu2, Yang Feng2, Wang Jing2, Liu Chang-sheng1, 2 
  

  1. 1Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China; 2Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China
  • Received:2016-09-22 Online:2016-12-16 Published:2016-12-16
  • Contact: Wang Jing, Associate professor, Master’s supervisor, Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China Liu Chang-sheng, Professor, Doctoral supervisor, Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China; Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China
  • About author:Ma Zheng-yu, Master, Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China
  • Supported by:

    the National Natural Science Foundation of China, No. 31330028, 31470923, 31271011

PDF

260

摘要：

文章快速阅读：

 

文题释义：
聚富马酸丙二醇酯：是一种主链上带有碳碳双键的线性可降解聚酯，在一定条件下可室温固化；另一方面，其降解产物为具有生物相容性的富马酸和丙二醇，可通过机体自身新陈代谢排出体外。
β-磷酸三钙：与骨无机成分类似，生物相容性好，降解较快，有利于成骨相关细胞长入，促进血管及新骨生成。β-磷酸三钙与高分子材料复合，作为性能改良剂通常用于增强高分子材料的力学性能、生物相容性和骨传导性，以及调节材料的降解性。

背景：聚富马酸丙二醇酯具有室温固化特性，β-磷酸三钙具有良好的生物相容性，但是目前缺乏聚富马酸丙二醇酯/β-磷酸三钙复合骨水泥的系统研究。
目的：制备聚富马酸丙二醇酯/β-磷酸三钙复合骨水泥，考察其体外生物活性和降解性。
方法：采用液相沉淀法制备β-磷酸三钙，两步法合成聚富马酸丙二醇酯高分子，制备可注射聚富马酸丙二醇酯/β-磷酸三钙复合骨水泥。①体外矿化：将聚富马酸丙二醇酯/β-磷酸三钙复合骨水泥、聚甲基丙烯酸甲酯骨水泥分别浸泡于模拟体液中7 d，检测材料表面羟基磷灰石沉积。②体外降解：将聚富马酸丙二醇酯/β-磷酸三钙复合骨水泥浸泡于PBS中，在相应的时间点检测吸水率、失重率、体系pH值、压缩强度及表面和断面形貌变化。
结果与结论：①体外矿化：可注射聚富马酸丙二醇酯/β-磷酸三钙复合骨水泥表面能够沉积羟基磷灰石，聚甲基丙烯酸甲酯骨水泥表面未沉积羟基磷灰石；②体外降解：聚富马酸丙二醇酯/β-磷酸三钙在PBS中63 d内体系pH值稳定，材料降解温和；84 d材料失重率为13.5%；扫描电镜显示聚富马酸丙二醇酯/β-磷酸三钙表面为溶蚀降解，压缩强度随着降解时间延长逐渐降低，有利于降解过程中力学性能的完整性和持续性；③结果表明：聚富马酸丙二醇酯/β-磷酸三钙复合骨水泥是一种具备体外矿化和降解能力的材料。

关键词: 生物材料, 骨生物材料, 聚富马酸丙二醇酯/β-磷酸三钙, 骨修复, 可注射, 生物活性, 体外降解, 国家自然科学基金

Abstract:

BACKGROUND: Poly(propylene fumarate) (PPF) can crosslink at room temperature, and β-tricalcium phosphate (β-TCP) has good biocompatibility, but PPF/β-TCP composite bone cement has not yet been systematically studied.
OBJECTIVE: To prepare PPF/β-TCP composite bone cement and to explore its in vitro bioactivity and degradability.
METHODS: β-TCP and PPF were respectively synthesized by liquid-phase precipitation and a two-step method, and PPF/β-TCP composite bone cement was prepared through mixing PPF with β-TCP. The    in vitro bioactivity of PPF/β-TCP was compared with the commercial poly(methyl methacrylate) (PMMA) through the ability of forming hydroxyapatite after immersed in simulated body fluid for 7 days. The in vitro degradability of PPF/β-TCP was studied via investigating the transformation of pH values, water uptake and mass loss, compressive strength and morphology at each time point.
RESULTS AND CONCLUSION: There were hydroxyapatites formed on the PPF/β-TCP material, but none on the commercial PMMA material. The pH values of the PPF/β-TCP were stable in PBS for 63 days, indicating its degradation is moderate; the mass loss was up to 13.5% after 84 days. Scanning electron microscope displayed the degraded PPF/β-TCP surface, and its compressive strength was decreased gradually, which good for the integrity and sustainability of mechanical properties during degradation. These results suggest that PPF/β-TCP bone cement holds mineralization and degradability in vitro.

Key words: Calcium Phosphates, Biodegradation, Environmental, Tissue Engineering

中图分类号: