中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (27): 4417-4422.doi: 10.3969/j.issn.2095-4344.2015.27.028

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

敲除H2-eb1基因建立的变应性鼻炎模型小鼠

李林格，冯  娟，胡  斌，寿  玺，张  春，田  钰，江春荣，张  瑜，张  华   

  1. 新疆医科大学第一附属医院耳鼻喉科，新疆维吾尔自治区乌鲁木齐市  830054
  • 出版日期:2015-06-30 发布日期:2015-06-30
  • 通讯作者: 张华，博士，教授，主任医师，新疆医科大学第一附属医院耳鼻喉科，新疆维吾尔自治区乌鲁木齐市 830054
  • 作者简介:李林格，汉族，2008年兰州大学毕业，主要从事变应性鼻炎方面的研究。
  • 基金资助:

    国家自然科学基金(81160125)

Establishing mouse models of allergic rhinitis by knocking out H2-eb1 gene 

Li Lin-ge, Feng Juan, Hu Bin, Shou Xi, Zhang Chun, Tian Yu, Jiang Chun-rong, Zhang Yu, Zhang Hua   

  1. Department of Otorhinolaryngology, the First Teaching Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Online:2015-06-30 Published:2015-06-30
  • Contact: Zhang Hua, M.D., Professor, Chief physician, Department of Otorhinolaryngology, the First Teaching Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • About author:Li Lin-ge, Department of Otorhinolaryngology, the First Teaching Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81160125

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摘要：

背景：HLA-DRB1与变应性鼻炎发病发病有关，构建HLA-DRB1基因敲除动物模型，不仅为阐明变应性鼻炎发病机制、同时也为相关疾病发病机制的阐明提供了良好的途径，然而未查阅到利用H2-eb1基因敲除小鼠进行相关研究的报道。
目的：构建HLA-DRB1基因敲除动物模型。
方法：经杂合子小鼠近亲繁殖，获得纯合子、野生型和杂合子小鼠。经基因及蛋白鉴定确认，采用随机数字表法选取8周龄雌性野生型(H2-eb1+/+)小鼠12只和H2-eb1-/-小鼠12只，将12只H2-eb1+/+小鼠和12只H2-eb1-/-小鼠以卵清蛋白致敏激发，建立小鼠变应性鼻炎模型。将另12只H2-eb1+/+小鼠以PBS代替卵清蛋白激发作为对照。
结果与结论：与对照小鼠相比，变应性鼻炎模型小鼠血清中卵清蛋白IgE、白细胞介素4水平明显升高，γ-干扰素水平明显降低；而与基因敲除野生型小鼠(H2-eb1+/+)相比，基因敲除H2-eb1-/-变应性鼻炎小鼠IgE、白细胞介素4水平较低，γ-干扰素水平较高。提示H2-eb1基因在变应性鼻炎的发病机制中的Th1/Th2失衡有重要调节作用。

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

关键词: 实验动物, 基因病毒载体及相关因子动物模型, 变态反应性疾病, 变应性鼻炎, 基因敲除, 基因型聚合酶链反应, 免疫印迹, 近亲繁殖, Th1/Th2平衡, 国家自然科学基金

Abstract:

BACKGROUND: HLA-DRB1 is related to the pathogenesis of allergic rhinitis. Construction of HLA-DRB1 gene knockout animal models not only elucidates the pathogenesis of allergic rhinitis, but also provides a good way for the elucidation of the pathogenesis of allergic rhinitis-related diseases.
OBJECTIVE: To establish the HLA-DRB1 gene knockout animal models.
METHODS: Homozygous, wild-type and heterozygous mice were obtained by inbreeding of the heterozygous mice. Confirmed by gene and protein identification, 24 female wild-type (H2-eb1+/+) mice and 12 H2-eb1-/- mice aged 8 weeks were selected according to the random number table. 12 H2-eb1+/+ mice and 12 H2-eb1-/- mice were sensitized with ovalbumin to establish the mouse models of allergic rhinitis. Another 12 mice were sensitized with PBS as comparison.
RESULTS AND CONCLUSION: Compared with the control mice, serum levels of ovalbumin-specific IgE and interleukin-4 were significantly increased, while serum level of γ-interferon was significantly decreased in the mouse models of allergic rhinitis. Serum levels of IgE and interleukin-4 were lower, while serumγ- interferon level was higher, in H2-eb1-/- gene knockout mice of allergic rhinitis than those in the H2-eb1+/+ gene knockout wild-type mice. These results suggest that H2-eb1 gene may play an important role in regulating Th1/Th2 imbalance in the pathogenesis of allergic rhinitis.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Rhinitis, Allergic, Perennial, Gene Knockout Techniques, Inbreeding, Th1/Th2 Balance

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