中国组织工程研究

中国组织工程研究 ›› 2025, Vol. 29 ›› Issue (2): 379-384.doi: 10.12307/2025.271

• 脊柱组织构建 spinal tissue construction • 上一篇    下一篇

改良结扎法用于脊髓损伤造模的优势分析

李道辉1，徐晓霜2，李郑涛1，田新鹏3，毕航川1，刘  源1，戴永文1，陈凌强1   

  1. 1昆明医科大学第一附属医院骨科，云南省昆明市  650032；2昆明医科大学公共卫生学院，云南省昆明市  650500；3西昌市人民医院，四川省凉山彝族自治州  615000
  • 收稿日期:2023-12-08 接受日期:2024-02-08 出版日期:2025-01-18 发布日期:2024-05-25
  • 通讯作者: 陈凌强，教授，博士生导师，昆明医科大学第一附属医院骨科，云南省昆明市 650032 共同通讯作者：戴永文，在读硕士，昆明医科大学第一附属医院骨科，云南省昆明市 650032
  • 作者简介:李道辉，男，1997年生，湖南省郴州市人，汉族，昆明医科大学第一附属医院在读硕士。 共同第一作者：徐晓霜，女，1999年生，重庆市人，汉族，昆明医科大学公共卫生学院在读硕士。
  • 基金资助:
    国家自然科学基金项目(81660215，81860093)，项目负责人：陈凌强；云南省科技厅科技计划项目(202101AT070226，202101AY070001-31)，项目负责人：陈凌强

Advantages of modified ligation method for spinal cord injury modeling 

Li Daohui1, Xu Xiaoshuang2, Li Zhengtao1, Tian Xinpeng3, Bi Hangchuan1, Liu Yuan1, Dai Yongwen1, Chen Lingqiang1   

  1. 1Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China; 2School of Public Health, Kunming Medical University, Kunming 650500, Yunnan Province, China; 3Xichang People’s Hospital, Liangshan Yi Autonomous Prefecture 615000, Sichuan Province, China
  • Received:2023-12-08 Accepted:2024-02-08 Online:2025-01-18 Published:2024-05-25
  • Contact: Chen Lingqiang, Professor, Doctoral supervisor, Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China Co-corresponding author: Dai Yongwen, Master candidate, Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
  • About author:Li Daohui, Master candidate, Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China Xu Xiaoshuang, Master candidate, School of Public Health, Kunming Medical University, Kunming 650500, Yunnan Province, China Li Daohui and Xu Xiaoshuang contributed equally to this work.
  • Supported by:
    National Natural Science Foundation of China, Nos. 81660215 and 81860093 (to CLQ); Science and Technology Programs of Yunnan Provincial Science and Technology Department, Nos. 202101AT070226 and 202101AY070001-31 (to CLQ)

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摘要：


文题释义：
基于压迫法改良的结扎法：压迫法是指以钳夹和球囊等压迫脊髓，形成脊髓挫伤和挤压伤的一种造模方式。基于压迫法改良的结扎法(后续均简称结扎法)是利用缝针和缝线将脊髓不完全结扎来模拟脊髓的挫伤和挤压伤的一种改良型造模方式。
排尿反射：是一种不自主的过程，膀胱容积信息被传递到中枢神经系统，特别是在导水管周围灰质被处理并传递到脑干脑桥中的巴林顿核(Bar，也称为脑桥排尿中心)，这种压力信息最终会达到阈值压力，从而触发排尿。 
排尿行为恢复：当脊髓损伤后，排尿反射由于失去高级中枢的易化作用，常常在脊髓损伤的急性期出现较为严重的尿潴留；排尿行为恢复则是大鼠脊髓损伤后适应失去高级中枢控制后由尿潴留转变为溢出性排尿。

背景：目前根据脊髓损伤的损伤方式不同衍生出不同的模型构建方法，传统物理损伤造模方法都有各自的优缺点，尚缺乏较为有效和稳定的脊髓损伤动物模型。
目的：建立一种可复制、可调控、创伤小、死亡率低、模型更稳定、适用范围广、术后护理时间短的脊髓损伤大鼠模型。
方法：将体质量和鼠龄相似的40只SD大鼠随机分为对照组和改良组，每组20只，对照组采用钳夹造模法构建脊髓损伤模型，改良组选用基于压迫法改良的结扎法，在开窗的基础上使用缝线结扎的方法进行脊髓损伤的造模。比较两组大鼠的排尿行为、血尿、脓尿(感染率)、死亡率、脊柱畸形率及术后1，3，5，7 d的BBB运动功能学评分。
结果与结论：基于压迫法改良的结扎法建立的脊髓损伤大鼠模型比常规建模方法排尿行为恢复较快、血尿率低、感染率低、死亡率低、脊柱畸形率低，BBB评分结果更集中稳定(1周内均 < 2分)，由此可证明基于压迫法改良的结扎法更适用于脊髓损伤大鼠模型的建立。

https://orcid.org/0009-0001-8945-8872(李道辉)；https://orcid.org/0009-0000-4442-6001(徐晓霜)
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 改良, 脊髓损伤模型, 钳夹造模法, 结扎法, 排尿反射, 排尿行为, 脊柱畸形, BBB评分

Abstract: BACKGROUND: Currently, different methods of model establishment have been derived from different injury modes of spinal cord injury. Traditional physical injury modeling methods have their own advantages and disadvantages, and there is a lack of more effective and stable animal models of spinal cord injury.
OBJECTIVE: To establish a reproducible, controllable, trauma-free, low-mortality, more stable, widely applicable, and short-term postoperative care rat model of spinal cord injury.
METHODS: Forty Sprague-Dawley rats with similar body mass and ages were randomly divided into a control group and an improved group, with 20 rats in each group. Animal models of spinal cord injury in the control group were constructed using a clip model method, while the improved group used a modified ligation method based on the compression method to make the spinal cord injury models using suture ligation based on fenestration. Postoperative comparisons were made between the two groups, assessing urination behavior, hematuria, pyuria (infection rate), mortality, scoliosis rate and Basso-Beattie-Bresnahan locomotor rating scale scores at 1, 3, 5, and 7 days after modeling.
RESULTS AND CONCLUSION: Compared with the conventional modeling method, the modified ligation method based on the compression method resulted in faster recovery of urination behavior, lower hematuria rate, lower infection rate, lower mortality rate, lower scoliosis rate, and more concentrated and stable Basso-Beattie-Bresnahan scores (all below 2 points within 1 week). This proves that the modified ligation method based on compression is more suitable for the establishment of spinal cord injury models in rats.

Key words: improvement, spinal cord injury model, clip model method, ligation method, urination reflex, urination behavior, scoliosis, BBB score

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