中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (30): 4912-4920.doi: 10.12307/2022.774
• 干细胞综述 stem cell review • 上一篇
吴梦鑫1,梁文红2,杨 琨3,韩盈盈4
收稿日期:
2021-08-13
接受日期:
2021-09-18
出版日期:
2022-10-28
发布日期:
2022-03-29
通讯作者:
韩盈盈,博士,副教授,遵义医科大学生命科学研究院口腔疾病研究特色重点实验室,贵州省遵义市 563000
作者简介:
吴梦鑫,男,1996 年生,四川省绵阳市人,汉族,遵义医科大学在读硕士,主要从事牙周组织再生方面的研究。
基金资助:
Wu Mengxin1, Liang Wenhong2, Yang Kun3, Han Yingying4
Received:
2021-08-13
Accepted:
2021-09-18
Online:
2022-10-28
Published:
2022-03-29
Contact:
Han Yingying, MD, Associate professor, Key Laboratory of Oral Diseases, Institute of Life Sciences, Zunyi Medical University, Zunyi 563000, Guizhou Province, China
About author:
Wu Mengxin, Master candidate, Hospital of Stomatology, Zunyi Medical University, Zunyi 563000, Guizhou Province, China
Supported by:
摘要:
文题释义:
牙周膜干细胞:又称牙周膜间充质细胞,是由Seo等在2004年首次分离出的一种独特的细胞群,易于获得,并显示出间充质干细胞的重要特性,如自我更新、多潜能和免疫调节。不仅在在牙周复合体的再生方面显示出潜力,而且在其他牙齿和非牙齿组织的再生方面也显示出了潜力。
牙周组织再生:牙周病变过程中牙周组织因炎症而破坏,如何获得牙周组织结构和功能的重建——即牙周组织再生,是牙周病研究领域中的重要课题。
背景:牙周病是一种牙齿支持组织的慢性炎症,这些组织的破坏导致牙齿缺失。牙周组织再生是牙周治疗的最终目标。
目的:综述牙周膜干细胞的生物学特性及其在促进牙周组织再生方面的各种影响因素。
方法:应用计算机检索PubMed、CNKI、万方、ScienceDirect、Medline数据库,以“Periodontal ligament stem cells,Periodontal tissue regeneration”及“牙周膜干细胞,牙周组织再生”为关键词,检索2004-2021年发表的相关文献。根据纳入排除标准筛选文献,最终纳入72篇文献进行综述。
结果与结论:牙周炎的特征是炎症导致牙齿支撑结构的进行性损害,直到牙齿脱落。牙周再生治疗的一个宏伟目标是修复牙周组织中丢失或受损的支持组织,包括牙槽骨、牙周膜和牙骨质,并可能有效减少牙周炎引起的牙齿丢失。干细胞用于牙周再生是目前再生研究的热点,也是牙周炎的一种潜在治疗方法。牙周膜干细胞可能是最适合作为细胞来源的细胞,生物学的深入研究也强调了牙周膜干细胞是很有前途的免疫调节剂。然而,有各种各样的影响因素增强或抑制牙周膜干细胞在牙周组织再生中所发挥的作用,了解各种因素对应的影响机制将有利于在此领域拥有更深入的研究。
缩略语:牙周膜干细胞:periodontal ligament stem cells,PDLSCs;基质细胞衍生因子1:stromal cell-derived factor 1,SDF-1
https://orcid.org/0000-0002-6780-1385 (吴梦鑫)
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
中图分类号:
吴梦鑫, 梁文红, 杨 琨, 韩盈盈. 牙周膜干细胞促进牙周组织再生的影响因素[J]. 中国组织工程研究, 2022, 26(30): 4912-4920.
Wu Mengxin, Liang Wenhong, Yang Kun, Han Yingying. Influencing factors of periodontal ligament stem cells promoting periodontal tissue regeneration[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(30): 4912-4920.
[1] LAUDENBACH JM, KUMAR SS. Common Dental and Periodontal Diseases. Dermatol Clin. 2020;38(4):413-420. [2] GBD 2016 Disease and Injury Incidence and Prevalence Collaborators. Global, regional, and national incidence, prevalence, and years lived with disability for 328 diseases and injuries for 195 countries, 1990-2016: a systematic analysis for the Global Burden of Disease Study 2016. Lancet. 2017;390(10100):1211-1259. [3] HERNÁNDEZ-MONJARAZ B, SANTIAGO-OSORIO E, MONROY-GARCÍA A, et al. Mesenchymal Stem Cells of Dental Origin for Inducing Tissue Regeneration in Periodontitis: A Mini-Review. Int J Mol Sci. 2018;19(4): 944. [4] AMANO A. Periodontal diseases and systemic diseases. Clin Calcium. 2017;27(10):1383-1391. [5] BARTOLD P M, GRONTHOS S, IVANOVSKI S, et al. Tissue engineered periodontal products. J Periodontal Res. 2016;51(1):1-15. [6] CHEN FM, JIN Y. Periodontal tissue engineering and regeneration: current approaches and expanding opportunities. Tissue Eng Part B Rev. 2010;16(2):219-255. [7] SEO BM, MIURA M, GRONTHOS S, et al. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet. 2004; 364(9429):149-155. [8] PARK CH, KIM KH, LEE YM, et al. Advanced Engineering Strategies for Periodontal Complex Regeneration. Materials(Basel). 2016;9(1):57. [9] GAY I C, CHEN S, MACDOUGALL M. Isolation and characterization of multipotent human periodontal ligament stem cells. Orthod Craniofac Res. 2007;10(3):149-160. [10] WADA N, MENICANIN D, SHI S, et al. Immunomodulatory properties of human periodontal ligament stem cells. J Cell Physiol. 2009;219(3): 667-676. [11] ZHANG J, AN Y, GAO L N, et al. The effect of aging on the pluripotential capacity and regenerative potential of human periodontal ligament stem cells. Biomaterials. 2012;33(29):6974-6986. [12] FORTINO VR, CHEN RS, PELAEZ D, et al. Neurogenesis of neural crest-derived periodontal ligament stem cells by EGF and bFGF. J Cell Physiol. 2014;229(4):479-488. [13] TANG HN, XIA Y, XU J, et al. Assessment of cellular materials generated by co-cultured ‘inflamed’ and healthy periodontal ligament stem cells from patient-matched groups. Exp Cell Res. 2016;346(1):119-129. [14] ABUARQOUB DA, ASLAM N, BARHAM RB, et al. The effect of platelet lysate in culture of PDLSCs: an in vitro comparative study. Peer J. 2019; 7:e7465. [15] TIAN Y, LIU M, LIU Y, et al. The performance of 3D bioscaffolding based on a human periodontal ligament stem cell printing technique. J Biomed Mater Res A. 2021;109(7):1209-1219. [16] SUAID FF, RIBEIRO FV, GOMES TR, et al. Autologous periodontal ligament cells in the treatment of Class III furcation defects: a study in dogs. J Clin Periodontol. 2012;39(4):377-384. [17] MENICANIN D, MROZIK KM, WADA N, et al. Periodontal-ligament-derived stem cells exhibit the capacity for long-term survival, self-renewal, and regeneration of multiple tissue types in vivo. Stem Cells Dev. 2014;23(9):1001-1011. [18] KOMAKI M. Pericytes in the Periodontal Ligament. Adv Exp Med Biol. 2019;1122:169-186. [19] HUANG CY, PELAEZ D, DOMINGUEZ-BENDALA J, et al. Plasticity of stem cells derived from adult periodontal ligament. Regen Med. 2009; 4(6):809-821. [20] KAWANABE N, MURATA S, MURAKAMI K, et al. Isolation of multipotent stem cells in human periodontal ligament using stage-specific embryonic antigen-4. Differentiation. 2010;79(2):74-83. [21] YAMASHITA YM, FULLER MT, JONES DL. Signaling in stem cell niches: lessons from the Drosophila germline. J Cell Sci. 2005;118(Pt 4): 665-672. [22] ELEUTERIO E, TRUBIANI O, SULPIZIO M, et al. Proteome of human stem cells from periodontal ligament and dental pulp. PLoS One. 2013;8(8): e71101. [23] SHI S, BARTOLD PM, MIURA M, et al. The efficacy of mesenchymal stem cells to regenerate and repair dental structures. Orthod Craniofac Res. 2005;8(3):191-199. [24] LIU J, LI Q, LIU S, et al. Periodontal Ligament Stem Cells in the Periodontitis Microenvironment Are Sensitive to Static Mechanical Strain. Stem Cells Int. 2017;2017:1380851. [25] MONNOUCHI S, MAEDA H, YUDA A, et al. Mechanical induction of interleukin-11 regulates osteoblastic/cementoblastic differentiation of human periodontal ligament stem/progenitor cells. J Periodontal Res. 2015;50(2):231-239. [26] LIU J, ZHAO Z, RUAN J, et al. Stem cells in the periodontal ligament differentiated into osteogenic, fibrogenic and cementogenic lineages for the regeneration of the periodontal complex. J Dent. 2020;92: 103259. [27] BUENO C, RAMIREZ C, RODRÍGUEZ-LOZANO FJ, et al. Human adult periodontal ligament-derived cells integrate and differentiate after implantation into the adult mammalian brain. Cell Transplant. 2013; 22(11):2017-2028. [28] BUENO C, MARTÍNEZ-MORGA M, MARTÍNEZ S. Non-proliferative neurogenesis in human periodontal ligament stem cells. Sci Rep. 2019; 9(1):18038. [29] NG TK, YANG Q, FORTINO VR, et al. MicroRNA-132 directs human periodontal ligament-derived neural crest stem cell neural differentiation. J Tissue Eng Regen Med. 2019;13(1):12-24. [30] IWASAKI K, KOMAKI M, AKAZAWA K, et al. Spontaneous differentiation of periodontal ligament stem cells into myofibroblast during ex vivo expansion. J Cell Physiol. 2019;234(11):20377-20391. [31] SHIN C, KIM M, HAN JA, et al. Human periodontal ligament stem cells suppress T-cell proliferation via down-regulation of non-classical major histocompatibility complex-like glycoprotein CD1b on dendritic cells. J Periodontal Res. 2017;52(1):135-146. [32] KUKOLJ T, TRIVANOVIĆ D, DJORDJEVIĆ IO, et al. Lipopolysaccharide can modify differentiation and immunomodulatory potential of periodontal ligament stem cells via ERK1,2 signaling. J Cell Physiol. 2018;233(1): 447-462. [33] MISAWA MYO, SILVÉRIO RUIZ KG, NOCITI FH JR, et al. Periodontal ligament-derived mesenchymal stem cells modulate neutrophil responses via paracrine mechanisms. J Periodontol. 2019;90(7): 747-755. [34] DU J, SHAN Z, MA P, et al. Allogeneic bone marrow mesenchymal stem cell transplantation for periodontal regeneration. J Dent Res. 2014; 93(2):183-188. [35] DAGHRERY A, FERREIRA JA, DE SOUZA ARAÚJO IJ, et al. A Highly Ordered, Nanostructured Fluorinated CaP-Coated Melt Electrowritten Scaffold for Periodontal Tissue Regeneration. Adv Healthc Mater. 2021; e2101152. [36] ZHOU M, GAO S, ZHANG X, et al. The protective effect of tetrahedral framework nucleic acids on periodontium under inflammatory conditions. Bioact Mater. 2021;6(6):1676-1688. [37] LIU S, WANG YN, MA B, et al. Gingipain-Responsive Thermosensitive Hydrogel Loaded with SDF-1 Facilitates In Situ Periodontal Tissue Regeneration. ACS Appl Mater Interfaces. 2021;13(31):36880-36893. [38] ISAAC A, JIVAN F, XIN S, et al. Microporous Bio-orthogonally Annealed Particle Hydrogels for Tissue Engineering and Regenerative Medicine. ACS Biomater Sci Eng. 2019;5(12):6395-6404. [39] AMMAR MM, WALY GH, SANIOUR SH, et al. Growth factor release and enhanced encapsulated periodontal stem cells viability by freeze-dried platelet concentrate loaded thermo-sensitive hydrogel for periodontal regeneration. Saudi Dent J. 2018;30(4):355-364. [40] SHEN S, ZHANG Y, ZHANG S, et al. 6-Bromoindirubin-3’-oxime Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells and Facilitates Bone Regeneration in a Mouse Periodontitis Model. ACS Biomater Sci Eng. 2021;7(1):232-241. [41] LAM L RW, SCHILLING K, ROMAS S, et al. Electrospun core-shell nanofibers with encapsulated enamel matrix derivative for guided periodontal tissue regeneration. Dent Mater J. 2021. doi: 10.4012/dmj.2020-412. [42] PENG W, REN S, ZHANG Y, et al. MgO Nanoparticles-Incorporated PCL/Gelatin-Derived Coaxial Electrospinning Nanocellulose Membranes for Periodontal Tissue Regeneration. Front Bioeng Biotechnol. 2021;9: 668428. [43] SHANG L, LIU Z, MA B, et al. Dimethyloxallyl glycine/nanosilicates-loaded osteogenic/angiogenic difunctional fibrous structure for functional periodontal tissue regeneration. Bioact Mater. 2021;6(4): 1175-1188. [44] IWASAKI K, AKAZAWA K, NAGATA M, et al. Angiogenic Effects of Secreted Factors from Periodontal Ligament Stem Cells. Dent J (Basel). 2021;9(1):9. [45] QIU J, WANG X, ZHOU H, et al. Enhancement of periodontal tissue regeneration by conditioned media from gingiva-derived or periodontal ligament-derived mesenchymal stem cells: a comparative study in rats. Stem Cell Res Ther. 2020;11(1):42. [46] XIONG X, YANG X, DAI H, et al. Extracellular matrix derived from human urine-derived stem cells enhances the expansion, adhesion, spreading, and differentiation of human periodontal ligament stem cells. Stem Cell Res Ther. 2019;10(1):396. [47] YU B, HU J, LI Q, et al. CircMAP3K11 Contributes to Proliferation, Apoptosis and Migration of Human Periodontal Ligament Stem Cells in Inflammatory Microenvironment by Regulating TLR4 via miR-511 Sponging. Front Pharmacol. 2021;12:633353. [48] YAN B, ZHANG H, DAI T, et al. Necrostatin-1 promotes ectopic periodontal tissue like structure regeneration in LPS-treated PDLSCs. PLoS One. 2018;13(11):e0207760. [49] YAN B, WEI K, HOU L, et al. Receptor-Interacting Protein 3/Caspase-8 May Regulate Inflammatory Response and Promote Tissue Regeneration in the Periodontal Microenvironment. Med Sci Monit. 2018;24:5247-5257. [50] XING Y, ZHANG Y, JIA L, et al. Lipopolysaccharide from Escherichia coli stimulates osteogenic differentiation of human periodontal ligament stem cells through Wnt/β-catenin-induced TAZ elevation. Mol Oral Microbiol. 2019;34(1): doi: 10.1111/omi.12249. [51] LIU L, LIU K, YAN Y, et al. Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells. Biomed Res Int. 2018;2018:7849294. [52] WANG H, LI J, ZHANG X, et al. Priming integrin alpha 5 promotes the osteogenic differentiation of human periodontal ligament stem cells due to cytoskeleton and cell cycle changes. J Proteomics. 2018; 179:122-130. [53] DUAN X, LIN Z, LIN X, et al. Study of platelet-rich fibrin combined with rat periodontal ligament stem cells in periodontal tissue regeneration. J Cell Mol Med. 2018;22(2):1047-1055. [54] GUO L, HOU Y, SONG L, et al. D-Mannose Enhanced Immunomodulation of Periodontal Ligament Stem Cells via Inhibiting IL-6 Secretion. Stem Cells Int. 2018;2018:7168231. [55] YAN W, CAO Y, YANG H, et al. CB1 enhanced the osteo/dentinogenic differentiation ability of periodontal ligament stem cells via p38 MAPK and JNK in an inflammatory environment. Cell Prolif. 2019;52(6): e12691. [56] AGHAMOHAMADI Z, KADKHODAZADEH M, TORSHABI M, et al. A compound of concentrated growth factor and periodontal ligament stem cell-derived conditioned medium. Tissue Cell. 2020;65:101373. [57] NIE F, ZHANG W, CUI Q, et al. Kaempferol promotes proliferation and osteogenic differentiation of periodontal ligament stem cells via Wnt/β-catenin signaling pathway. Life Sci. 2020;258:118143. [58] TAKEUCHI T, MASUNO K, UMEDA M, et al. Palmitate induces apoptosis and inhibits osteogenic differentiation of human periodontal ligament stem cells. Arch Oral Biol. 2020;112:104681. [59] ZHANG R, LIANG Q, KANG W, et al. Metformin facilitates the proliferation, migration, and osteogenic differentiation of periodontal ligament stem cells in vitro. Cell Biol Int. 2019; doi: 10.1002/cbin. 11202. [60] YANG S, GUO L, SU Y, et al. Nitric oxide balances osteoblast and adipocyte lineage differentiation via the JNK/MAPK signaling pathway in periodontal ligament stem cells. Stem Cell Res Ther. 2018;9(1):118. [61] SHI B, SHAO B, YANG C, et al. Upregulation of JHDM1D-AS1 protects PDLSCs from H(2)O(2)-induced apoptosis by decreasing DNAJC10 via phosphorylation of eIF2α. Biochimie. 2019;165:48-56. [62] FU X, FENG Y, SHAO B, et al. Activation of the ERK/Creb/Bcl‑2 pathway protects periodontal ligament stem cells against hydrogen peroxide‑induced oxidative stress. Mol Med Rep. 2019;19(5): 3649-3657. [63] FENG Y, FU X, LOU X. Notch pathway deactivation mediated by F-box/WD repeat domain-containing 7 ameliorates hydrogen peroxide-induced apoptosis in rat periodontal ligament stem cells. Arch Oral Biol. 2019;100:93-99. [64] WANG Y, LI J, QIU Y, et al. Low‑intensity pulsed ultrasound promotes periodontal ligament stem cell migration through TWIST1‑mediated SDF‑1 expression. Int J Mol Med. 2018;42(1):322-330. [65] LI H, ZHOU J, ZHU M, et al. Low-intensity pulsed ultrasound promotes the formation of periodontal ligament stem cell sheets and ectopic periodontal tissue regeneration. J Biomed Mater Res A. 2021;109(7): 1101-1112. [66] YAMAUCHI N, TAGUCHI Y, KATO H, et al. High-power, red-light-emitting diode irradiation enhances proliferation, osteogenic differentiation, and mineralization of human periodontal ligament stem cells via ERK signaling pathway. J Periodontol. 2018;89(3):351-360. [67] HASEGAWA D, HASEGAWA K, KANEKO H, et al. MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells. Stem Cells Int. 2020;2020:9672673. [68] ZHANG J, ZHANG C, YANG H, et al. Depletion of PRDM9 enhances proliferation, migration and chemotaxis potentials in human periodontal ligament stem cells. Connect Tissue Res. 2020;61(5):498-508. [69] YANG X, XIONG X, ZHOU W, et al. Effects of human urine-derived stem cells on the cementogenic differentiation of indirectly-cocultured periodontal ligament stem cells. Am J Transl Res. 2020;12(2):361-378. [70] RAMENZONI LL, RUSSO G, MOCCIA MD, et al. Periodontal bacterial supernatants modify differentiation, migration and inflammatory cytokine expression in human periodontal ligament stem cells. PLoS One. 2019;14(7):e0219181. [71] CHEN FM, GAO LN, TIAN BM, et al. Treatment of periodontal intrabony defects using autologous periodontal ligament stem cells: a randomized clinical trial. Stem Cell Res Ther. 2016;7:33. [72] SáNCHEZ N, FIERRAVANTI L, NúñEZ J, et al. Periodontal regeneration using a xenogeneic bone substitute seeded with autologous periodontal ligament-derived mesenchymal stem cells: A 12-month quasi-randomized controlled pilot clinical trial. J Clin Periodontol. 2020; 47(11):1391-1402. |
[1] | 范亚茹, 李瑞欣, 李凤集, 罗睿, 刘浩, 严颖彬. 载吲哚菁绿聚乳酸-羟基乙酸共聚物微球的表征及其光热效应 [J]. 中国组织工程研究, 2022, 26(在线): 1-6. |
[2] | 姚晓玲, 彭建城, 许岳荣, 杨志东, 张顺聪. 可变角度零切迹前路椎间融合内固定系统治疗脊髓型颈椎病:30个月随访[J]. 中国组织工程研究, 2022, 26(9): 1377-1382. |
[3] | 朱 婵, 韩栩珂, 姚承佼, 周 倩, 张 强, 陈 秋. 人体唾液成分与骨质疏松/骨量低下[J]. 中国组织工程研究, 2022, 26(9): 1439-1444. |
[4] | 金 涛, 刘 林, 朱晓燕, 史宇悰, 牛建雄, 张同同, 吴树金, 杨青山. 骨关节炎与线粒体异常[J]. 中国组织工程研究, 2022, 26(9): 1452-1458. |
[5] | 张立创, 徐 浩, 马迎辉, 熊梦婷, 韩海慧, 鲍嘉敏, 翟伟韬, 梁倩倩. 免疫调控淋巴回流功能治疗类风湿关节炎的机制及前景[J]. 中国组织工程研究, 2022, 26(9): 1459-1466. |
[6] | 王 景, 熊 山, 曹 金, 冯林伟, 王 信. 白细胞介素3在骨代谢中的作用及机制[J]. 中国组织工程研究, 2022, 26(8): 1260-1265. |
[7] | 朱 婵, 韩栩珂, 姚承佼, 张 强, 刘 静, 邵 明. 针刺治疗帕金森病:动物实验显示的作用机制[J]. 中国组织工程研究, 2022, 26(8): 1272-1277. |
[8] | 安维政, 何 萧, 任 帅, 刘建宇. 肌源干细胞在周围神经再生中的潜力[J]. 中国组织工程研究, 2022, 26(7): 1130-1136. |
[9] | 范一鸣, 刘方煜, 张洪宇, 李 帅, 王岩松. 脊髓损伤后室管膜区内源性神经干细胞反应的系列问题[J]. 中国组织工程研究, 2022, 26(7): 1137-1142. |
[10] | 黄晨玮, 费彦亢, 朱梦梅, 李鹏昊, 于 兵. 谷胱甘肽在干细胞“干性”及调控中的重要作用[J]. 中国组织工程研究, 2022, 26(7): 1119-1124. |
[11] | 惠小珊, 白 京, 周思远, 王 阶, 张金生, 何庆勇, 孟培培. 中医药调控干细胞诱导分化的理论机制[J]. 中国组织工程研究, 2022, 26(7): 1125-1129. |
[12] | 田 川, 朱向情, 杨再玲, 鄢东海, 李 晔, 王严影, 杨育坤, 何 洁, 吕冠柯, 蔡学敏, 舒丽萍, 何志旭, 潘兴华. 骨髓间充质干细胞调控猕猴卵巢的衰老[J]. 中国组织工程研究, 2022, 26(7): 985-991. |
[13] | 郭 嘉, 丁琼桦, 刘 泽, 吕思懿, 周泉程, 高玉花, 白春雨. 间充质干细胞来源外泌体的生物学特性及免疫调控作用[J]. 中国组织工程研究, 2022, 26(7): 1093-1101. |
[14] | 吴玮玥, 郭晓东, 包崇云. 工程化外泌体在骨修复再生中的应用[J]. 中国组织工程研究, 2022, 26(7): 1102-1106. |
[15] | 周洪琴, 吴丹丹, 杨 琨, 刘 琪. 传递特定miRNA的外泌体可调控成骨并促进成血管[J]. 中国组织工程研究, 2022, 26(7): 1107-1112. |
牙周炎是最常见的口腔疾病之一,如果不治疗可能会对个体产生不可逆转的后遗症和整体心理和生理影响,从而降低生活质量。晚期牙周病导致牙齿脱落的负担很重[1]。根据全球疾病负担研究(2016),严重的牙周病是世界上第11位最流行的疾病[2]。牙周炎会持续破坏牙周组织,如果不治疗会导致牙齿脱落[3]。牙周炎还与各种系统性疾病的发生和预后密切相关,包括心血管疾病、癌症、肥胖、糖尿病和慢性肾炎等[4]。因此,探索有效、安全、可转化为临床的牙周治疗方法是世界各国迫切的健康需求。牙周治疗的宏伟目标是再生多种牙周组织,包括受损牙周组织中的牙槽骨、牙骨质和牙周膜(PDL)[5]。虽然非手术牙周治疗可以通过物理方法去除病原体和坏死组织来防止疾病进展,但在治疗部位只有少量牙周组织可以再生[3]。引导组织再生(GTR)等技术在牙周手术中的应用可以不规则地修复牙槽骨和软组织,但总体结果并不一定令人满意,而且缺乏临床预见性[6]。尽管新的生物材料和生长因子丰富了牙周缺损的治疗方法,但临床试验表明它们的疗效仍然存在争议,丢失牙周组织的结构和功能再生仍然具有挑战性[5]。
干细胞可以自我更新和分化为多种细胞类型,因此具有巨大的治疗潜力。2004年,从人牙周组织中鉴定出干细胞,称为牙周膜干细胞(periodontal ligament stem cells,PDLSCs),开创了牙周再生研究的新纪元[7]。从那时起,人们发现其他干细胞在适当的诱导条件下具有形成多个牙周组织的能力。除了干细胞的再生能力外,干细胞接受免疫调节的能力在获得成功的结果中也起着同样重要的作用。目前,干细胞的使用被认为是牙周治疗的主流策略,特别是对于牙周复合体的完全再生,这不仅意味着重建合适的牙槽骨,而且还意味着通过定向插入新形成的牙周膜组织来诱导牙根表面的牙骨质形成[8]。
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
1.1.8 检索文献量 共检索到文献808篇,其中英文文献425篇,中文文献383篇。
1.2 文献筛选标准
1.2.1 纳入标准 有关牙周膜干细胞介绍的文献;与牙周组织再生有关的文献;有关牙周膜干细胞在牙周组织再生方面的文献
1.2.2 排除标准 ①与该文主题不相干的文章;②重复性研究;③较为陈旧的文章。
1.3 质量评估及数据的提取 共检索到 808篇文献,通过阅读文章标题及摘要进行初步筛选,资料收集者共同评估相关文献的重复性、非相关性、陈旧性;通读全文内容后,将72篇文献纳入并进行综述。文献检索流程,见图2。
牙周组织再生疗法是通过应用一些生物材料、生长因子和细胞而发展起来的。目前,关于这一疗法的研究主要集中在探讨牙周膜干细胞的再生能力上,研究已有十余年,但至今仍局限在动物模型和细胞研究上,缺乏相关的临床研究。作者通过查阅文献,仅发现2篇文献对PDLSCs应用于临床的安全性和可行性进行了临床研究,并证实其是安全的且不会产生不良反应,但与对照组(不含PDLSCs的治疗组)相比,实验组(含PDLSCs的治疗组)未检测到具有统计学意义的差异[71-72]。而且以此篇综述所引用的文章看,研究主要偏向于PDLSCs促进牙周组织再生的影响因素,甚少研究PDLSCs抑制牙周组织再生的影响因素,前人的综述主要集中在PDLSCs的再生潜力、动物模型研究等,而此次综述从一个不同的角度——影响因素入手,探讨促进或抑制PDLSCs影响牙周组织再生的影响因素,总结了各影响因素对PDLSCs在牙周组织再生方面的促进或抑制作用,为更多的临床研究铺路。然而各种各样的影响因素太多,故此次综述以近两年最新的研究文章为主,揭示了该领域的研究趋势,为后来的研究人员提供研究思路。目前来看,这一领域的基础研究还有很长的路要走。为了确保临床应用的安全性、重复性和成本效益,制定更好的培养、储存、扩增和分化PDLSCs的方案是一项挑战。
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
文题释义:
牙周膜干细胞:又称牙周膜间充质细胞,是由Seo等在2004年首次分离出的一种独特的细胞群,易于获得,并显示出间充质干细胞的重要特性,如自我更新、多潜能和免疫调节。不仅在在牙周复合体的再生方面显示出潜力,而且在其他牙齿和非牙齿组织的再生方面也显示出了潜力。
牙周组织再生:牙周病变过程中牙周组织因炎症而破坏,如何获得牙周组织结构和功能的重建——即牙周组织再生,是牙周病研究领域中的重要课题。
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
ScienceDirect数据库(www.ScienceDirect.com),是荷兰一家全球著名的学术期刊出版商,每年出版大量的学术图书和期刊,大部分期刊被SCI、SSCI、EI收录,是世界上公认的高品位学术期刊。 #br# Medline数据库(ovidsp.dc2.ovid.com),是美国国立医学图书馆(The National Library of Medicine,简称NLM)生产的国际性综合生物医学信息书目数据库,是当前国际上最权威的生物医学文献数据库。
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||