中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (36): 5787-5792.doi: 10.3969/j.issn.2095-4344.2017.36.009

• 骨组织构建 bone tissue construction • 上一篇    下一篇

低浓度木瓜蛋白酶诱导兔早期膝骨关节炎滑膜炎症的时间曲线

张康乐1，郭珈宜2，刘  源3，范仪铭4，高  山4，李  峰2，郭艳幸2   

  1. 1明光市中医院，安徽省滁州市  239400；2河南省洛阳正骨医院，河南省洛阳市  471000；3河南中医药大学，河南省郑州市  450000；4湖南中医药大学，湖南省长沙市  410000
  • 收稿日期:2017-07-09 出版日期:2017-12-28 发布日期:2018-01-04
  • 通讯作者: 郭艳幸，博士生导师，教授，主任医师，河南省洛阳正骨医院河南省骨科医院，河南省洛阳市 471000
  • 作者简介:张康乐，男，1990年生，汉族，医师，于明光市中医院工作。
  • 基金资助:

    河南省科技厅项目(112102310008)；国家中医药管理局“平乐郭氏正骨流派传承工作室”建设项目([2012]228)；河南省中医药管理局“河南省中医临床学科领军人才培育计划”项目(HNZYLJ20131009)

Synovial inflammation of a rabbit model of early-stage knee osteoarthritis induced by low concentration of papain at different time points

Zhang Kang-le1, Guo Jia-yi2, Liu Yuan3, Fan Yi-ming4, Gao Shan4, Li Feng2, Guo Yan-xing2   

  1. 1Mingguang Hospital of Traditional Chinese Medicine, Chuzhou 239400, Anhui Province, China; 2Luoyang Orthopedic Hospital of Henan Province, Luoyang 471000, Henan Province, China; 3Henan University of Chinese Medicine, Zhengzhou 450000, Henan Province, China; 4Hunan University of Chinese Medicine, Changsha 410000, Hunan Province, China
  • Received:2017-07-09 Online:2017-12-28 Published:2018-01-04
  • Contact: Guo Yan-xing, Doctoral supervisor, Professor, Chief physician, Luoyang Orthopedic Hospital of Henan Province, Luoyang 471000, Henan Province, China
  • About author:Zhang Kang-le, Physician, Mingguang Hospital of Traditional Chinese Medicine, Chuzhou 239400, Anhui Province, China
  • Supported by:

    the Project of Henan Provincial Department of Science and Technology, No. 112102310008; the Project of the State Administration of Traditional Chinese Medicine of China, No. [2012]228; the “Leading Talent Training Plan in Clinical Medicine of Henan Province” by the Traditional Chinese Medicine Administration of Henan Province, No. HNZYLJ20131009

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被引次数

1

摘要：

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文题释义：
木瓜蛋白酶：是一种蛋白水解酶，在酸性、中性、碱性环境下均能分解蛋白。木瓜蛋白酶在关节内可以溶解关节软骨，从而刺激滑膜组织炎症，产生一系列骨关节炎、滑膜炎症状，在实验研究中常用于制备骨关节炎动物模型。
滑膜炎症：是由多种原因引起滑膜组织增生、分泌增多、炎性细胞增多，滑膜炎症在骨关节炎的发生发展中有重要作用，与骨关节炎的形成密切相关。滑膜炎症时滑液中炎性介质分泌增加，打破关节内原有的平衡，加重软骨破坏，反过来软骨的损伤又能刺激滑膜炎的分泌。所以在研究骨关节炎的同时对滑膜炎症要有充足的认识。
摘要
背景：膝关节滑膜炎症在早期膝骨关节炎的发生发展中起重要作用。 
目的：比较低浓度木瓜蛋白酶不同诱导时间下兔膝骨关节炎滑膜炎症的变化，为研究膝关节滑膜炎提供数据支持。
方法：将24只新西兰大耳白兔随机分为4组，分别在第1，4，7天右膝关节注入2%的木瓜蛋白酶和      0.03 mol/L L-半胱氨酸混合液0.5 mL，在末次注射后第1，2，3周将兔子分批处死，空白对照组在第3周处死，记录兔膝关节局部皮温和周径，并取滑膜及髌下脂肪垫组织行病理学观察及酶联免疫吸附测定。
结果与结论：①在造模后第1周有大量关节积液，膝关节局部皮温及周径均高于第2，3周；②滑膜组织中白细胞介素1、肿瘤坏死因子α、基质金属蛋白酶13浓度在第1，2，3周均高于空白组，且第1周时浓度最高，第2，3周差异无显著性意义；③滑膜组织形态学显示，第1，2，3周滑膜组织均增生、变厚，有炎性细胞浸润，随着造模时间的延长，滑膜组织中血管增生明显增加；④说明低浓度木瓜蛋白酶和0.03 mol/L L-半胱氨酸混合在1周内能形成兔膝关节滑膜炎症，并产生大量关节积液，随时间增加滑膜组织增厚、血管增生明显，关节积液减少。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0003-3039-5923(张康乐)

关键词: 组织构建, 骨组织工程, 膝关节, 骨关节炎, 滑膜炎症, 木瓜蛋白酶, 动物模型

Abstract:

BACKGROUND: Synovitis plays an important role in the occurrence and development of early knee osteoarthritis.
OBJECTIVE: To compare synovial inflammation in a rabbit mode of knee osteoarthritis induced by injecting low concentration of papain at different time points, thus providing reference for the study on synovitis in knee osteoarthritis.
METHODS: Twenty-four New Zealand white rabbits were divided randomly into four groups, and received the 
injection of 0.5 mL mixture of 2% papain with 0.03 mol/L L-cysteine into the right knee at 1, 4 and 7 days, respectively. The model rabbits were respectively sacrificed at 1, 2 and 3 weeks after the last injection, and the rabbits in the blank control group were killed at 3 weeks. The local skin temperature and circumference of the knee were recorded, and the synovium and infrapatellar fat pad were separated from the right knees for histopathological observation and ELSA.
RESULTS AND CONCLUSION: At the 1st week after modeling, joint effusion was significantly increased, local skin temperature and circumference of knee joint were higher than those at the 2nd, and 3rd weeks. The levels of interleukin-1, tumor necrosis factor-α and matrix metalloproteinase-13 in the synovium in the three experimental groups were higher than those in the blank control group at 1, 2 and 3 weeks after modeling; the levels peaked in the 1st week, but no significant fluctuation appeared in the 2nd and 3rd weeks. There were synovial tissue hyperplasia, thickening, and inflammatory cell infiltration in the 1st, 2nd and 3rd weeks, and the proliferation of synovial tissue increased significantly with time. These findings indicate that the intra-articular injection of low concentration of papain and 0.03 mol/L L-cysteine mixtures contributes to a rabbit model of knee synovial inflammation within 1 week, with significantly joint effusion. However, significant synovial tissue thickening and vascular hyperplasia are observed; meanwhile, the joint effusion is decreased obviously.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoarthritis, Knee, Synovitis, Papain, Models, Animal, Tissue Engineering

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