中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (44): 6667-6672.doi: 10.3969/j.issn.2095-4344.2016.44.019

• 骨与关节临床实践 clinical practice of the bone and joint • 上一篇    下一篇

细胞膜微粒CD31、CD54与酒精性股骨头缺血性坏死的关系：随机对照实验方案

杨 云1，范海燕2，黄 健1，马忠平1，张志峰1   

  1. 1内蒙古医科大学第二附属医院，内蒙古自治区呼和浩特市 010030；2内蒙古医科大学附属医院，内蒙古自治区呼和浩特市 010030
  • 修回日期:2016-08-16 出版日期:2016-10-28 发布日期:2016-10-28
  • 通讯作者: 黄健，博士，主任医师，内蒙古医科大学第二附属医院，内蒙古自治区呼和浩特市 010030
  • 作者简介:杨云，男，1980年生，内蒙古自治区包头市人，汉族，2013年内蒙古医科大学毕业，硕士，主治医师，主要从事骨与关节疾病诊断及治疗研究。 并列第一作者：范海燕，女，1980年生，内蒙古自治区呼和浩特市人，汉族，2015年内蒙古医科大学毕业，硕士，主治医师，主要从事骨与关节疾病影像学诊断研究。
  • 基金资助:

    内蒙古自治区卫生和计划生育委员会医疗卫生科研项目(201301061)；内蒙古医科大学青年创新基金项目(YKD2015QNCX028)

Relationship of cell membrane microparticles CD31 and CD54 with alcohol-induced avascular necrosis of the femoral head: study protocol for a randomized controlled trial

Yang Yun1, Fan Hai-yan2, Huang Jian1, Ma Zhong-ping1, Zhang Zhi-feng1   

  1. 1Second Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China; 2Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
  • Revised:2016-08-16 Online:2016-10-28 Published:2016-10-28
  • Contact: Huang Jian, M.D., Chief physician, Second Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
  • About author:Yang Yun, Master, Attending physician, Second Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China Fan Hai-yan, Master, Attending physician, Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China Yang Yun and Fan Hai-yan contributed equally to this paper.
  • Supported by:

    the Medical and Health Research Project of the Inner Mongolia Autonomous Region Health and Family Planning Commission, No. 201301061; the Youth Innovation Fund Project of Inner Mongolia Medical University, No. YKD2015QNCX028

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摘要：

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文题释义：
细胞膜微粒：是血管内皮损伤后从细胞表面脱落的一种小的膜性囊泡状物质，由磷脂和蛋白构成，直径在0.1-1 μm，主要由血小板和内皮细胞释放。
细胞膜微粒CD31、CD54的功能：细胞膜微粒起源不同，作用不同。CD54由内皮源性微粒特异表达，CD31由血小板及内皮细胞源性微粒共同表达；CD31、CD54可损伤血管内皮细胞，启动内源性凝血，影响血管舒张功能；CD31表面富含血小板因子、组织因子和黏附因子，可黏附于暴露的内皮下胶原；同时CD31还含有凝血因子，可为凝血酶原的激活提供更大的催化面积。因此细胞膜微粒CD31、CD54可通过介导血管炎症反应、促凝、影响血管舒缩及促进血管内皮损伤导致股骨头内微血管损伤。
 
摘要
背景：细胞膜微粒CD31、CD54可通过介导血管炎症反应、促凝、影响血管舒缩及促进血管内皮损伤导致股骨头内微血管损伤。文献已证实细胞膜微粒在激素性股骨头坏死中发挥重要作用，但是目前尚未见微粒与酒精性股骨头缺血性坏死相关性的研究。
方法/设计：随机对照动物实验。健康雄性Wistar大鼠随机分为2组，模型组连续6个月灌胃给予烈性白酒制备酒精性股骨头缺血性坏死模型，空白对照组给予等量生理盐水灌胃。干预1-6个月，每月2组各随机取6只大鼠，分批取血，流式细胞仪检测血清细胞膜微粒CD31、CD54水平；取双侧股骨头，固定、脱钙、石蜡包埋切片，行苏木精-伊红染色光镜下进行空骨陷窝计数确定股骨头坏死情况，再行Verhoeff 氏染色及MSB微血栓染色观察股骨头内微血管损伤及微血栓形成情况；并分析细胞膜微粒CD31、CD54水平与股骨头坏死、血管内皮损伤及微血栓形成的相关性。
讨论：实验结果拟探讨细胞膜微粒CD31、CD54在酒精性股骨头坏死中的作用，更加深入探讨酒精性股骨头坏死发病机制，对其早期诊断、早期治疗提供新的理论依据，可能为其治疗提供一种新的靶点。
伦理批准：实验方案经内蒙古医科大学动物实验伦理委员会批准，批准号为YKD2016154。大鼠的实验操作和取材遵循《关于善待实验动物的指导性意见》的规定，并与美国国立卫生与健康研究院的指南一致。

中国组织工程研究杂志出版内容重点：人工关节；骨植入物；脊柱；骨折；内固定；数字化骨科；组织工程

ORCID: 0000-0002-5382-4252(杨云)

关键词: 组织构建, 骨组织工程, 细胞膜微粒, 酒精性股骨头坏死

Abstract:

BACKGROUND: Cell membrane microparticles CD31 and CD54 lead to microvascular injury in the femoral head by mediating vascular inflammatory response, promoting blood clotting, affecting vasomotion and promoting vascular endothelial injury. Studies have verified that membrane particles play an important role in steroid-induced necrosis of the femoral head, but there is no studies concerning relationship between microparticles and alcohol-induced avascular necrosis of femoral head.

METHODS/DESIGN: This is a randomized controlled animal study. Healthy male Wistar rats will be randomly assigned to two groups. In the model group, rats will be intragastrically administered hard liquor for 6 consecutive months to prepare models of alcohol-induced avascular necrosis of the femoral head. Blank controls will be intragastrically given an equal volume of physiological saline. In 1-6 months of intervention, six rats will be randomly selected from each group every month. Blood will be collected separately. Flow cytometry will be used to detect serum cell membrane particles CD31, CD54 levels. Bilateral femoral head will be fixed, decalcified, embedded in wax, and then sections. After hematoxylin-eosin staining, empty bone lacuna will be quantified under a light microscope to identify femoral head necrosis. Verhoff’s staining and MSB microthrombosis staining will be used to observe microvascular injury and microvascular thrombosis in the femoral head, and to analyze the correlation of CD31 and CD54 levels with femoral head necrosis, vascular endothelial injury and microvascular thrombosis.
DISCUSSION: This study will investigate the effects of CD31 and CD54 on alcohol-induced avascular necrosis of the femoral head, explore the pathogenesis of alcohol-induced avascular necrosis of the femoral head, provide a new theoretical basis for early diagnosis and early treatment, and may provide a new target for its treatment.
ETHICS APPROVAL: The protocol has been approved by the Animal Experimental Ethics Committee of Inner Mongolia Medical University (approval number YKD2016154). Experimental procedures and materials of rats will be in accordance with the Guide for the Care and Use of Laboratory Animals, which is consistent with the guide of National Institutes of Health. 

中国组织工程研究杂志出版内容重点：人工关节；骨植入物；脊柱；骨折；内固定；数字化骨科；组织工程

Key words: Femur Head Necrosis, Membrane Proteins, Tissue Engineering

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