中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (42): 6737-6741.doi: 10.3969/j.issn.2095-4344.2014.42.003

• 骨组织构建 bone tissue construction • 上一篇    下一篇

去卵巢骨质疏松大鼠骨组织和骨髓细胞FoxO1蛋白在全身垂直振动条件下的变化

文思敏1，2，卜淑敏1，高梦锦2   

  1. 1首都体育学院，北京市  100191
    2山西大学商务学院，山西省太原市  030031
  • 修回日期:2014-09-09 出版日期:2014-10-08 发布日期:2014-10-08
  • 通讯作者: 卜淑敏，教授，硕士生导师，首都体育学院，北京市 100191
  • 作者简介:文思敏，女，1987年生，山西省襄汾县人，汉族，首都体育学院在读硕士，主要从事运动生理生化理论与应用的研究。
  • 基金资助:

    北京市市属高等院校长城学者项目资助(CIT&TCD20130337)

Effects of whole-body vertical vibration on the protein expression of FoxO1 in bone tissue and bone marrow cells of ovariectomized osteoporosis rats

Wen Si-min1, 2, Bu Shu-min1, Gao Meng-jin2   

  1. 1Capital University of Physical Education and Sports, Beijing 100191, China
    2Business College of Shanxi University, Taiyuan 030031, Shanxi Province, China
  • Revised:2014-09-09 Online:2014-10-08 Published:2014-10-08
  • Contact: Bu Shu-min, Professor, Master’s supervisor, Capital University of Physical Education and Sports, Beijing 100191, China
  • About author:Wen Si-min, Studying for master’s degree, Capital University of Physical Education and Sports, Beijing 100191, China
  • Supported by:

    Project of Great Wall Scholar, Beijing Municipal Commission of Education, No. CIT&TCD20130337

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摘要：

背景：FoxO1蛋白参与多种多样的细胞和生理过程，包括细胞增殖与凋亡、活性氧的反应、长寿、癌症、细胞周期和代谢的调节等。研究认为振动负荷能促进新陈代谢，延缓肌肉疲劳，加速骨适应性重建。
目的：进一步验证全身垂直振动对去卵巢骨质疏松大鼠骨组织和骨髓细胞叉头框转录因子O亚族1(FoxO1)蛋白表达的影响。
方法：将36只健康3月龄雌性SD大鼠，按体质量分层后随机分为假手术组、去卵巢静止组和去卵巢振动组。在手术后第11周，对去卵巢振动组大鼠实施7周每天不间断的全身垂直振动治疗，2次/d，每次振动训练15 min，间隔10 min，振动频率为90 Hz，振动幅度为0.5 mm。于末次处理结束24-48 h内，游离各组大鼠第5腰椎、右侧股骨和胫骨，用双能X射线骨密度仪检测右股骨和第5腰椎的离体骨密度，用Western blot检测右胫骨近端和骨髓细胞FoxO1蛋白的表达。
结果与结论：骨密度检测结果显示，与去卵巢静止组比较，去卵巢振动组大鼠股骨远端和近端以及第5腰椎骨密度显著增加，而股骨中段无显著变化。Western blot检测结果显示：FoxO1蛋白在胫骨组织中不表达，但在骨髓细胞中表达。与假手术组比较，去卵巢静止组大鼠骨髓细胞FoxO1蛋白表达水平无显著变化；与去卵巢静止组比较，去卵巢振动组大鼠骨髓细胞FoxO1蛋白表达水平显著下降。结果表明，全身垂直振动能抑制FoxO1蛋白在去卵巢大鼠骨髓细胞中的表达。



中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

关键词: 组织构建, 骨组织工程, 全身垂直振动, 去卵巢骨质疏松大鼠, 绝经后骨质疏松, 叉头框转录因子O亚族1(FoxO1)

Abstract:

BACKGROUND: FoxO1 participates in diverse processes of cell physiology, including cell proliferation and apoptosis, reactive oxygen species, longevity, cancer, the regulation of cell cycle and metabolism. Vibration loading is believed to promote metabolism, delay muscle fatigue, and facilitate adaptive bone reconstruction.
OBJECTIVE: To investigate the effects of whole-body vertical vibration on the protein expression of FoxO1 in tibia and bone marrow cell of ovariectomized (OVX) osteoporosis rats.
METHODS: Thirty-six healthy 3-month-old female Sprague-Dawley rats were randomly divided into the following three groups by body weight: sham-operation (sham) group, OVX group, and OVX whole-body vertical vibration (OVX+V) group. At the 11th week after operation, the OVX+V group rats were vibrated on a vibration platform twice per day for 7 weeks according to the following schedule: whole-body vertical vibration for 15 minutes, with an interval of 10 minutes. The frequency was 90 Hz and the amplitude was 0.5 mm. Within 24-48 hours after the last treatment, the fifth lumbar vertebra as well as the right femur and tibia were isolated. The bone mineral density of the fifth lumbar vertebra and right femur was detected by dual-energy Χ-ray absorptiometry. The FoxO1 protein expression in proximal tibia and bone marrow cells was detected by western blot assay.
RESULTS AND CONCLUSION: As revealed by the results of bone mineral density, the bone mineral densities of the proximal and distal femur as well as the fifth lumbar vertebra in OVX+V group were significantly increased while no significant difference could be seen in the mid shaft. As revealed by the results of western blot, there was no FoxO1 protein expression in the tibia, but it could be detected in the bone marrow cells. Compared with the sham group, there was no significant difference in FoxO1 protein expression in bone marrow cells of OVX group; compared with the OVX group, the protein expression level of FoxO1 in bone marrow cells of the OVX+V group was significantly reduced. The results suggested that whole-body vertical vibration could inhibit the protein expression of FoxO1 in bone marrow cells of osteoporosis rats.



中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

Key words: vibration, ovariectomy, osteoporosis, postmenopausal

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