中国组织工程研究

中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (8): 1362-1366.doi: 10.3969/j.issn.2095-4344.2013.08.006

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

神经营养因子3纳米微球载体基因工程转染许旺细胞

宗海斌1，贾金领2，董玉珍2，周慧聪2   

  1. 1新乡医学院基础医学院机能实验室，河南省新乡市 453003
    2 新乡医学院第一附属医院骨外科，河南省卫辉市 453100
  • 收稿日期:2012-04-21 修回日期:2012-05-30 出版日期:2013-02-19 发布日期:2013-03-23
  • 通讯作者: 董玉珍，博士，副主任医师，副教授，新乡医学院第一附属医院骨外科，河南省卫辉市 453100 dongyuzhen1998@163.com
  • 作者简介:宗海斌，男，1969年生，上海市人，汉族，1992年河南理工大学毕业，讲师，主要从事神经损伤后的再生与康复研究。 laohai0044@163.com

Transfection of Schwann cells with neurotrophic factor-3 nanoparticles

Zong Hai-bin1, Jia Jin-ling2, Dong Yu-zhen2, Zhou Hui-cong2   

  1. 1 Skill Laboratory of Basic Medical Science of Xinxiang Medical College, Xinxiang 453003, Henan Province, China
    2 Department of Orthopedics, the First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan Province, China
  • Received:2012-04-21 Revised:2012-05-30 Online:2013-02-19 Published:2013-03-23
  • Contact: Dong Yu-zhen, Doctor, Associate chief physician, Associate professor, Department of Orthopedics, the First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan Province, China dongyuzhen1998@163.com
  • About author:Zong Hai-bin, Lecturer, Skill Laboratory of Basic Medical Science of Xinxiang Medical College, Xinxiang 453003, Henan Province, China laohai0044@163.com

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摘要：

背景：纳米微球基因转染技术携带或增强种植细胞可持续稳定延长其释放，保持其活性和作用时间。
目的：观察纳米微球载体转染神经营养因子3基因修饰的许旺细胞对坐骨神经缺损的促进和修复作用。
方法：采用纳米微球载体将神经营养因子3基因转染入体外培养的新生Wistar大鼠许旺细胞。取Wistar成年大鼠80只制作坐骨神经缺损模型，将4种不同的移植物注入细胞外基质凝胶-聚乳酸聚羟基乙酸共聚物管桥接管内：空白对照组未注入其他任何物质，细胞组注入许旺细胞，基因组注入神经营养因子3基因，实验组注入神经营养因子3基因修饰的许旺细胞。
结果与结论：术后坐骨神经及肌纤维横截面积染色比较，实验组优于细胞组、基因组(P < 0.01)，细胞组、基因组均优于空白对照组(P < 0.01)，细胞组、基因组组间比较差异无显著性意义(P > 0.05)。表明神经营养因子3基因转染许旺细胞移植，可相互促进，再造神经再生的微环境，促进并修复缺损坐骨神经缺损。

关键词: 生物材料, 纳米生物材料, 基因工程, 纳米微球载体, 神经营养因子3, 许旺细胞, 神经缺损, 省级基金, 生物材料图片文章

Abstract:

BACKGROUND: The technology of nanoparticle carrier transferring gene can carry or enhance plant cell
sustainable stability, extend its release and maintain its activity and duration.
OBJECTIVE: To explore the effects of nanoparticle carrier with neurotrophic factor-3 on transfection of Schwann cells in facilitating and repairing sciatic nerve defects.
METHODS: The neurotrophic factor-3 genes were transfected to the Schwann cells of newborn Wistar rats with nanoparticle carrier. Eighty adult Wistar rats were selected to make the sciatic nerve defect model. Then four kinds of grafts were injected into the pipe bridge takeover of extracellular matrix gel-poly( lactide-co-glycolide): the blank control group was not injected with other substances, the cell group was injected with Schwann cells, the gene group was injected with neurotrophic factor-3 gene, and the experimental group was injected with neurotrophic factor-3 gene modified Schwann cells.
RESULTS AND CONCLUSION: The staining of sciatic nerve and muscle fiber cross-sectional area was better in the experimental group than in the cell group and gene group (P < 0.01), which was better in the cell and gene groups than the blank control group (P < 0.01); but, there was no significant difference between the cell group and gene group (P > 0.05). It indicated that neurotrophic factor-3 gene modified Schwann cells transplantation can reconstruct the microenvironment of nerve regeneration and promote repair of sciatic nerve defects.

Key words: biomaterials, nano-biomaterials, genetic engineering, nanoparticle carrier, neurotrophic factor-3, Schwann cells, nerve defects, provincial grants-supported paper, biomaterial photographs-containing paper

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