中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (43): 8005-8008.doi: 10.3969/j.issn.2095-4344.2012.43.006

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

聚磷酸钙纤维/羟基磷灰石/明胶软骨组织工程支架材料的制备及性能

王彦平，朱凌云，张红梅   

  1. 兰州交通大学机电工程学院材料科学与工程系，甘肃省兰州市 730070
  • 收稿日期:2012-01-14 修回日期:2012-03-11 出版日期:2012-10-21 发布日期:2013-04-08
  • 通讯作者: 王彦平，硕士，副教授，兰州交通大学机电学院材料系，甘肃省兰州市 730070 wangyp@ mail.lzjtu.cn
  • 作者简介:王彦平★，男，1974年生，甘肃省会宁县人，汉族，2003年兰州交通大学毕业，硕士，副教授，主要从事生物医用材料研究。 wangyp@ mail.lzjtu.cn

Preparation and properties of calcium polyphosphate fiber/hydroxyapatite/gelatin scaffolds for cartilage tissue engineering

Wang Yan-ping, Zhu Ling-yun, Zhang Hong-mei   

  1. Department of Materials Science and Engineering, School of Mechatronic Engineering, Lanzhou Jiaotong University, Lanzhou 730070, Gansu Province, China
  • Received:2012-01-14 Revised:2012-03-11 Online:2012-10-21 Published:2013-04-08
  • Contact: Wang Yan-ping★, Master, Associate professor, Department of Materials Science and Engineering, School of Mechatronic Engineering, Lanzhou Jiaotong University, Lanzhou 730070, Gansu Province, China wangyp@ mail.lzjtu.cn
  • About author:Wang Yan-ping★, Master, Associate professor, Department of Materials Science and Engineering, School of Mechatronic Engineering, Lanzhou Jiaotong University, Lanzhou 730070, Gansu Province, China wangyp@ mail.lzjtu.cn

PDF

741

被引次数

9

摘要：

背景：目前明胶基组织工程支架材料存在力学性能低、生物相容性差、降解速率难以控制等缺陷。
目的：通过添加聚磷酸钙纤维和羟基磷灰石改善明胶支架材料的性能。
方法：以自制聚磷酸钙纤维和羟基磷灰石为添加材料，明胶为基体材料，以戊二醛为交联剂，采用溶媒浇铸/粒子滤取技术制备配比为50/10/40的聚磷酸钙纤维/羟基磷灰石/明胶软骨组织工程支架复合材料。测试支架材料的物理力学性能，并观察其微观结构。
结果与结论：采用溶胶凝胶法制得的羟基磷灰石粉末结晶程度较差，经900 ℃下煅烧0.5 h后，可制得结晶程度较高的羟基磷灰石粉末。聚磷酸钙纤维/羟基磷灰石/明胶软骨组织工程支架材料具有三维、连通、微孔网状空间结构，孔隙率在65%-90%之间，满足软骨组织工程对其支架材料孔隙的要求。戊二醛的交联作用和聚磷酸钙纤维的增强作用，克服了明胶在制备多孔支架时容易收缩的缺点，制得高孔隙率三维连通的支架材料。

关键词: 软骨, 组织工程, 明胶, 聚磷酸钙纤维, 羟基磷灰石, 支架材料, 生物材料

Abstract:

BACKGROUND: Nowadays, the gelatin scaffold for tissue engineering has many disadvantages, such as low stiffness and strength, bad biocompatibility and uncontrollable degradation rate.
OBJECTIVE: To improve the properties of gelatin scaffold by adding calcium polyphosphate fibers and hydroxyapatite.
METHODS: Calcium polyphosphate fiber and hydroxyapatite were taken as reinforced materials and gelatin was used as matrix materials, besides, glutaric dialdehyde was used as the cross linker in a ratio of 50/10/40. The composite scaffolds for cartilage tissue engineering were prepared by solvent-casting and particulate-leaching technique, and then their physical and mechanical properties as well as microstructure were tested.
RESULTS AND CONCLUSION: The hydroxyapatite powder prepared by sol-gel method had a low crystallization degree, but it had a high crystallization degree after sintered at 900 ℃ for 0.5 hour. The scaffolds with porosity of 65%-90% possessed three-dimensional, interconnecting, micro-hole network structure, and can meet the porosity require of cartilage tissue engineering for scaffolds. The effect of glutaraldehyde cross-linking and improvement of calcium polyphosphate fiber can overcome the disadvantage of easily shrinking of gelatin when the porous scaffold scaffolds are prepared, and provide promise to prepare a high-porosity scaffold material which has three-dimensional and interconnection structure.

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