中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (28): 5160-5164.doi: 10.3969/j.issn.2095-4344.2012.28.007

• 肌肉肌腱韧带组织构建 tissue construction of the muscle, tendon and ligament • 上一篇    下一篇

前列腺素E2干预后交叉韧带成纤维细胞和滑膜细胞的迁移

陈荣富1，王春莉2，谢 静2，黄 伟1，梁 熙1，宋国立2, 3○，符纯锋1，陈 诚1   

  1. 1重庆医科大学附属第一医院骨科，重庆市 400016；
    2重庆大学生物工程学院，重庆市 400030；
    3加州大学圣迭亚哥分校骨科系及生物工程系，美国加利福尼亚州 CA92093-0412
  • 收稿日期:2012-03-07 修回日期:2012-05-07 出版日期:2012-07-08 发布日期:2012-07-08
  • 通讯作者: 黄伟，男，1968年生，博士后，教授，主任医师，硕士生导师，重庆医科大学附属第一医院骨科，重庆市 400016 huangwei68huangwei@163.com
  • 作者简介:陈荣富★，男，1986年生，四川省乐至县人，汉族， 2012年重庆医科大学毕业，硕士，医师，从事骨关节疾病等方面的研究与骨科临床工作。 271500790@qq.com

Effects of prostaglandin E2 on the migration of posterior cruciate ligament fibroblasts and synovial cells

Chen Rong-fu1, Wang Chun-li2, Xie Jing2, Huang Wei1, Liang Xi1, KL Paul Sung2, 3○, Fu Chun-feng1, Chen Cheng1   

  1. 1Department of Orthopedics, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;
    2College of Bioengineering, Chongqing University, Chongqing 400030, China;
    3Department of Orthopedics and Bioengineering, University of California, Califonia CA 92093-0412, USA
  • Received:2012-03-07 Revised:2012-05-07 Online:2012-07-08 Published:2012-07-08
  • Contact: Huang Wei, Doctor, Professor, Chief physician, Master’s supervisor, Department of Orthopedics, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China huangwei68huangwei @163.com
  • About author:Chen Rong-fu★, Master, Physician, Department of Orthopedics, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China 271500790@qq.com

PDF

379

摘要：

背景：细胞迁移是组织损伤后修复过程中的重要环节之一，但是膝关节交叉韧带损伤后局部产生的细胞因子前列腺素E2对后交叉韧带成纤维细胞和滑膜细胞迁移的影响尚未见报道。
目的：在后交叉韧带成纤维细胞与滑膜细胞单培养和共培养的条件下，研究前列腺素E2对此2种细胞迁移的影响。
方法：体外培养人后交叉韧带成纤维细胞和滑膜细胞，分别进行2种细胞的单培养和Transwell共培养，并用质量浓度10 μg/L的前列腺素E2干预。比较单培养和共培养下细胞生长状态；细胞划痕试验检测24 h时细胞的迁移率。
结果与结论：共培养使后交叉韧带成纤维细胞和滑膜细胞的迁移率较单培养增高128%和48%。前列腺素E2分别使单培养下后交叉韧带成纤维细胞和滑膜细胞的迁移率降低了28%和14%；使共培养下细胞的迁移率较相应的共培养组未处理细胞下降37%和24%。证实，在前列腺素E2的刺激下，后交叉韧带成纤维细胞和滑膜细胞迁移率均明显降低，尤其对共培养下后交叉韧带成纤维细胞迁移率的抑制更显著。

关键词: 细胞迁移, 后交叉韧带, 共培养, 滑膜细胞, 前列腺素E2, 组织构建

Abstract:

BACKGROUND: Cells migration is one of the important steps in the process of wound repair. But the effects of prostaglandin E2 on the migration of posterior cruciate ligament fibroblasts and synovial cells remain unclear.
OBJECTIVE: To observe the effects of prostaglandin E2 on the migration of posterior cruciate ligament fibroblasts and synovial cells under mono-culture and Transwell co-culture in vitro.
METHODS: Posterior cruciate ligament fibroblasts and synovial cells were mono-cultured and co-cultured by Transwell. Then the mono-cultured cells or co-cultured cells were treated by 10 μg/L prostaglandin E2. Cell growth status was observed and compared. Cell migration was assessed by wound healing assay at 24 hours after treatment.
RESULTS AND CONCLUSION: The migration rates of posterior cruciate ligament fibroblasts and synovial cells under co-culture were increased by 128% and 48% compared with the mono-cultured cells. Prostaglandin E2 decreased the migration rates of the mono-cultured posterior cruciate ligament fibroblasts and synovial cells by 28% and 14% compared with the mono-culture control, and also decreased the migration rates of the co-cultured cells by 37% and 24% compared with the co-culture group. These results indicate that prostaglandin E2 inhibited the migration of both posterior cruciate ligament fibroblasts and synovial cells, especially stronger in co-cultured posterior cruciate ligament fibroblasts.

中图分类号: