中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (25): 4561-4565.doi: 10.3969/j.issn.1673-8225.2012.25.001

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

脱基质小牛骨粉复合骨髓基质干细胞的成骨作用

李 祥1，黄代营2，陈松龄2，朱双喜1   

  1. 中山大学附属第一医院，1黄埔院区口腔科，2口腔科，广东省广州市 510080
  • 收稿日期:2011-12-06 修回日期:2012-02-04 出版日期:2012-06-17 发布日期:2013-11-04
  • 通讯作者: 陈松龄，男，博士，教授，博士生导师，中山大学附属第一医院口腔科，广东省广州市 510080 chensongling@ hotmail.com
  • 作者简介:李祥☆，男，1971年出生，安徽省蚌埠市人，汉族，2010年中山大学博士毕业，主治医师，主要从事牙种植方面的研究。 hpyylx@163.co
  • 基金资助:

    广东省科技厅科研基金(2009B050700025)；广州市黄埔区科技局项目(WS11001)

Osteogenetic effect of Bio-Oss bone powder combined with bone marrow stromal stem cells

Li Xiang1, Huang Dai-ying2, Chen Song-ling2, Zhu Shuang-xi1   

  1. 1Department of Stomatology, Huangpu Branch, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China;
    2Department of Stomatology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
  • Received:2011-12-06 Revised:2012-02-04 Online:2012-06-17 Published:2013-11-04
  • Contact: Chen Song-ling, Doctor, Professor, Doctoral supervisor, Department of Stomatology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China chensongling@ hotmail.com
  • About author:Li Xiang☆, Doctor, Attending physician, Department of Stomatology, Huangpu Branch, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China hpyylx@163.com

PDF

451

被引次数

3

摘要：

背景：利用骨组织工程技术在上颌窦提升中的成骨研究是目前口腔种植学到研究热点。
目的：探讨骨组织工程在上颌窦底提升同期牙种植中的成骨效果。
方法：体外分离培养犬骨髓基质干细胞，将细胞与脱基质小牛骨粉复合培养并向成骨细胞定向诱导分化。健康成年犬12只行双侧上颌窦底提升同期牙种植，一侧植入复合物，另一侧植入脱基质小牛骨粉做对照。
结果与结论：种植体稳固无松动，上颌窦黏膜完整。实验侧可见新生骨形成较早、骨量较多；对照侧新生骨形成较慢。X射线显示实验侧新生骨质致密，与种植体结合紧密。随时间的增加，牵出力增大，12，24周时两侧差异有显著性意义(P < 0.05)。组织形态学检测显示新生骨面积逐渐增加，12，24周时差异有显著性意义(P < 0.05)。提示组织工程化骨在上颌窦提升同期牙种植中可获得良好的成骨效果。

关键词: 小牛骨粉, 骨髓基质干细胞, 牙种植, 上颌窦提升, 骨组织工程, 生物材料

Abstract:

BACKGROUND: The use of bone tissue engineering in maxillary sinus augmentation is a research hotspot in oral implantology at present.
OBJECTIVE: To investigate the osteogenic effect of bone tissue engineering on maxillary sinus floor augmentation with simultaneous dental implant.
METHODS: The bone marrow stromal stem cells were isolated from dogs and cultured in vitro. Then, the cells were cultured with Bio-Oss bone powder and induced to differentiate to osteoblasts. Twelve healthy adult dogs were subjected to bilateral maxillary sinus elevation with simultaneous dental implant. One side was implanted with the compound of bone marrow stromal stem cells and Bio-Oss bone power, and the other side was implanted with Bio-Oss bone power alone as controls.
RESULTS AND CONCLUSION: Gross observation showed that the implants were stable and not loose, as well as maxillary sinus mucosa was intact. New bone at the experimental side formed earlier and increased in bone mass, while new bone at the control side formed more slowly. X-ray examination showed that new bone at the experimental side was dense and combined tightly with implants. Biomechanical testing indicated that the pull-out force was increased with time, and there were significant differences between the experimental and control sides at weeks 12 and 24 (P < 0.05). Histomorphological analysis exhibited that new bone area was increased gradually, and there were significant differences between the experimental and control sides at weeks 12 and 24 (P < 0.05). These findings suggest that the tissue engineered bone can obtain a good effect on maxillary sinus augmentation with simultaneous dental implant.

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