中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (20): 3634-3638.doi: 10.3969/j.issn.1673-8225.2012.20.006

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

大鼠膝关节软骨与不同强度跑台运动的影响*★

詹荔琼1，党  娜2，高美钦3，倪国新1, 4   

  1. 福建医科大学，  1康复治疗学系运动与康复研究室，2生理及病理生理学系，3肿瘤研究室，福建省福州市350004；4南方医科大学南方医院创伤骨科，广东省广州市
    510515
  • 收稿日期:2011-09-27 修回日期:2011-10-26 出版日期:2012-05-13 发布日期:2012-05-13
  • 通讯作者: 倪国新，教授，南方医科大学南方医院创伤骨科，广东省广州市 510515 nigxlx@gmail.com guoxinni@yahoo.com
  • 作者简介:詹荔琼★，女，1986年生，福建省莆田市人，汉族，2011年福建医科大学毕业，硕士，主要从事运动与骨性关节炎的发生研究。 zhanlq08@163.com
  • 基金资助:

    福建医科大学教授基金项目资助(2007BS004)。

Effects of treadmill running exercise with different intensity on articular cartilage in rats  

Zhan Li-qiong1, Dang Na2, Gao Mei-qin3, Ni Guo-xin1, 4   

  1. 1Laboratory of Exercise and Rehabilitation, Department of Rehabilitation Medicine, 2Department of Physiology and Pathophysiology, 3Laboratory of Experimental Tumor, Fujian Medical University, Fuzhou 350004, Fujian Province, China; 4Department of Orthopedics and Trauma, Nanfang Hospital, Southern Medical University, Guangzhou  510515, Guangdong Province, China
  • Received:2011-09-27 Revised:2011-10-26 Online:2012-05-13 Published:2012-05-13
  • Contact: Ni Guo-xin, Professor, Laboratory of Exercise and Rehabilitation, Fujian Medical University, Fuzhou 350004, Fujian Province, China; Department of Rehabilitation Medicine, Department of Orthopedics and Trauma, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China nigxlx@gmail.com guoxinni@yahoo.com
  • About author:Zhan Li-qiong★, Master, Laboratory of Exercise and Rehabilitation, Department of Rehabilitation Medicine, Fujian Medical University, Fuzhou 350004, Fujian Province, China zhanlq08@163.com
  • Supported by:

    the Professor Foundation of Fujian Medical University, No. 2007BS004*

PDF

512

被引次数

12

摘要：

背景：适量的运动是维持正常关节组织形态结构及生理功能的必要条件，过度运动或制动均可导致关节软骨退变。
目的：观察不同强度跑台运动对大鼠膝关节软骨的影响。
方法：将18只雄性成年Wistar大鼠随机分安静组、低强度运动组、高强度运动组。6周后 ELISA法测定血清基质金属蛋白酶3水平，并行蕃红-O染色、基质金属蛋白酶3与Ⅱ型胶原免疫组织化学染色及Mankin评分，反转录聚合酶链反应检测软骨基质金属蛋白酶3的mRNA表达。
结果与结论：高强度运动组Mankin评分、血清及软骨中基质金属蛋白酶3表达均显著高于安静组与低强度运动组(P < 0.05)，基质糖氨多糖及Ⅱ型胶原含量均显著低于安静组与低强度运动组(P < 0.05)；低强度运动组与安静组差异无显著性意义。说明高强度运动可造成大鼠膝关节软骨退行性变，且软骨运动性损伤可能与基质金属蛋白酶3表达增强有关。
 

关键词: 跑台运动, 软骨, 关节软骨, 基质金属蛋白酶3, 运动强度

Abstract:

BACKGROUND: Moderate activities are necessary to maintain the normal structure, histological morphology and physiological function of joints. Excessive exercise and immobility lead to articular cartilage degeneration.
OBJECTIVE: To explore the effects of treadmill running exercise with different intensity on articular cartilage of rat knee joints.
METHODS: A total of 18 male adult Wistar rats were randomly assigned into three groups: sedentary group, low intensity exercise group and high intensity exercise group. Serum samples were collected to measure matrix metalloproteinase 3 content using enzyme linked immunosorbent assay. In addition, cartilage characteristics were observed by safranin-O staining. Matrix metalloproteinase 3 and type Ⅱ collagen were stained by immunohistochemical staining, and were evaluated by Mankin’s histological grading system. The mRNA expression of matrix metalloproteinase 3 in cartilage was detected by reverse transcription-PCR.
RESULTS AND CONCLUSION: Compared with sedentary group and low intensity exercise group, significant differences were found in high intensity exercise group in terms of Mankin’s score, matrix metalloproteinase 3 concentrations in the serum and cartilage (P < 0.05). The contents of matrix glycosaminoglycan and type Ⅱ collagen in high intensity exercise group were significantly lower than those in the sedentary group and low intensity exercise group (P < 0.05). There was no significant difference between sedentary group and low intensity exercise group. These results indicate that high intensity exercise can lead to articular cartilage degeneration in rat knees, and exercise-induced cartilage injury may be related to the increasing expression of matrix metalloproteinase 3.
 

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