中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (16): 2875-2878.doi: 10.3969/j.issn.1673-8225.2012.16.006

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

金纳米链及抗表皮生长因子受体抗体/金纳米链共轭物近红外热疗可启动  细胞凋亡通路*☆

丛林海，彭淑昆，何晓光，杨一兵   

  1. 昆明医学院第一附属医院耳鼻咽喉科，云南省昆明市  650031
  • 收稿日期:2012-01-09 修回日期:2012-02-08 出版日期:2012-04-15 发布日期:2012-04-15
  • 通讯作者: 何晓光，博士，教授，昆明医学院第一附属医院耳鼻咽喉科，云南省昆明市 650031 hexg1018@ yahoo.com.cn
  • 作者简介:丛林海☆，男，1972年生，黑龙江省伊春市人，汉族，2011年昆明医学院毕业，博士，主治医师，主要从事头颈肿瘤基础及临床研究。conglinhai2008@126.com 并列第一作者：彭淑昆，女，云南省昆明市人，汉族，1986年昆明医学院毕业，主管技师。
  • 基金资助:

    国家自然科学基金项目(81160324/H1625)，课题名称：EGFR-mAb功能化修饰金纳米棒光热诱导Hep-2细胞调往分子机制研究。

Gold nanochain and anti-epidermal growth factor receptor/Au near-infrared hyperthermia can start apoptosis pathway

Cong Lin-hai, Peng Shu-kun, He Xiao-guang, Yang Yi-bing   

  1. Department of Otorhinolaryngology, First Affiliated Hospital of Kunming Medical University, Kunming  650031, Yunnan Province, China
  • Received:2012-01-09 Revised:2012-02-08 Online:2012-04-15 Published:2012-04-15
  • Contact: author: He Xiao-guang, Doctor, Professor, Department of Otorhinolaryngology, First Affiliated Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China hexg1018@ yahoo.com.cn
  • About author:Cong Lin-hai☆, Doctor, Attending physician, Department of Otorhinolaryngology, First Affiliated Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China Conglinhai2008@ 126.com Peng Shu-kun, Technician in charge, Department of Otorhinolaryngology, First Affiliated Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No.81160324/H1625*

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摘要：

背景：金纳米颗粒在近红外激光照射下对肿瘤细胞具有杀伤效应。
目的：评价金纳米链及抗表皮生长因子受体抗体/金纳米链共轭物近红外热疗对人喉癌Hep-2细胞的热杀伤作用并探讨其凋亡通路。
方法：取生长良好的人喉鳞癌Hep-2细胞，分别应用金纳米链或抗表皮生长因子受体抗体/金纳米链共轭物溶液进行干预，在此基础上应用8 W/cm2近红外激光(808 nm)照射6 min，以单独应用1640培养液培养及单独给予近红外激光照射的细胞作对照。
结果与结论：透射电镜观察发现，金纳米链热疗后Hep-2细胞有明显的损伤，抗表皮生长因子受体抗体/金纳米链热疗后细胞的损伤程度最重。Western blot检测显示，金纳米链热疗和抗表皮生长因子受体抗体/金纳米链热疗后细胞热休克蛋白70和促凋亡蛋白Bax表达明显增高，而抑凋亡蛋白Bcl-2表达明显降低。说明金纳米链及抗表皮生长因子受体抗体/金纳米链共轭物近红外热疗可通过启动细胞凋亡通路杀伤人喉癌Hep-2细胞。
关键词：金纳米链；抗表皮生长因子受体抗体；Hep-2细胞；热疗；凋亡；生物材料；纳米技术
缩略语注释：EGFR：epidermal growth factor receptor，表皮生长因子受体；Hsp70：heat shock protein 70，热休克蛋白70
doi:10.3969/j.issn.1673-8225.2012.16.006

关键词: 金纳米链, 抗表皮生长因子受体抗体, Hep-2细胞, 热疗, 凋亡, 生物材料, 纳米技术

Abstract:

BACKGROUND: Gold nanoparticles have a killing effect on tumor cells under the near-infrared laser irradiation.
OBJECTIVE: To evaluate the killing effect of gold nanochain and anti-epidermal growth factor receptor (EGFR)/Au conjugates in near-infrared hyperthermia on human laryngeal cancer Hep-2 cells and to explore relevant apoptosis pathway.
METHODS: Human laryngeal squamous carcinoma Hep-2 cells were extracted and intervened in gold nanochain or anti-EGFR/Au solution. Then, the Hep-2 cells were exposed to the near-infrared laser irradiation of 8 W/cm2 (808 nm) for       6 minutes. The cells cultured alone in 1640 culture medium or only exposed to near-infrared laser were as controls.
RESULTS AND CONCLUSION: Under transmission electron microscope, the Hep-2 cells cultured in the gold nanochain solution after hyperthermia were significantly damaged, and the cells in the anti-EGFR/Au solution after hyperthermia were damaged worst. The Western blot detection exhibited that the expressions of heat-shock protein 70 and pro-apoptotic protein Bax were significantly increased, while the expression of anti-apoptotic protein Bcl-2 was obviously decreased exposed to gold nanochain and anti-EGFR/Au near-infrared hyperthermia. These suggest that gold nanochain and anti-EGFR/Au near-infrared hyperthermia can kill human laryngeal cancer Hep-2 cells via apo-ptosis pathway activation.

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