中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (41): 7653-7656.doi: 10.3969/j.issn.1673-8225.2011.41.013

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

MG63和MG63/ADR骨肉瘤裸鼠模型血管内皮生长因子和核因子κB1的表达

孙  佼1，张春林2，吴小三3，赵世昌2，赵  曜2，曾炳芳2   

  1. 1苏州大学医学部上海市第六人民医院骨科，上海市  200233
    2上海交通大学附属第六人民医院骨科，上海市  200233
    3六安市人民医院骨科，安徽省六安市  237005
  • 收稿日期:2011-04-01 修回日期:2011-06-06 出版日期:2011-10-08 发布日期:2011-10-08
  • 通讯作者: 张春林，副主任医师，硕士生导师，上海交通大学附属第六人民医院骨科，上海市 200233 shzhangchunlin@gmail.com
  • 作者简介:孙佼★，男，1985年生，山东省淄博市人，汉族，硕士，医师，主要从事骨创伤修复及骨肿瘤骨病的研究。 Sun8516@163.com
  • 基金资助:

    国家自然科学基金资助项目(30700850)。

Vascular endothelial growth factor and nuclear factor-kappa B1 expression in a nude mouse model of MG63 and MG63/ADR osteosarcoma

Sun Jiao1, Zhang Chun-lin2, Wu Xiao-san3, Zhao Shi-chang2, Zhao Yao2, Zeng Bing-fang2   

  1. 1Department of Orthopedics, Shanghai Sixth People's Hospital, School of Medicine， Soochow University, Shanghai  200233, China
    2Department of Orthopedics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai  200233, China
    3Department of Orthopedics, Liuan People's Hospital, Liuan  237005, Anhui Province, China
  • Received:2011-04-01 Revised:2011-06-06 Online:2011-10-08 Published:2011-10-08
  • Contact: Zhang Chun-lin, Associate chief physician, Department of Orthopedics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200233, China shzhangchunlin@ gmail.com
  • About author:Sun Jiao★, Studying for master’s degree, Physician, Department of Orthopedics, Shanghai Sixth People's Hospital, School of Medicine, Soochow University, Shanghai 200233, China sun8516@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30700850*

PDF

378

摘要：

背景：恶性肿瘤耐药研究中发现核因子κB1及血管内皮生长因子基因可能与肿瘤耐药密切相关。
目的：观察MG63和MG63/ADR骨肉瘤裸鼠模型中血管内皮生长因子和核因子κB1表达的差异。
方法：对数期生长的MG63细胞采用阿霉素浓度递增培养法冲击诱导建立MG63/ADR细胞株。BALB/C裸鼠随机分为2组，分别将含5.0×106个MG63和MG63/ADR细胞的0.2 mL细胞悬液采用皮下接种法注入裸鼠的一侧腋部皮下。第6周处死裸鼠取材。
结果与结论：注射MG63和MG63/ADR细胞的2组裸鼠成瘤率均为100%；免疫组织化学及RT-PCR方法检测发现MG63/ADR组瘤灶中血管内皮生长因子和核因子κB1基因和蛋白的表达均明显高于MG63组(P < 0.05)。说明血管内皮生长因子和核因子κB1表达的上调可能是导致骨肉瘤耐药的重要原因。

关键词: 阿霉素, 耐药, 肿瘤, 血管内皮生长因子, 核因子&kappa, B1

Abstract:

BACKGROUND: Studies concerning sarcoma drug resistance found that vascular endothelial growth factor (VEGF) and nuclear factor-kappa B1 (NF-κB1) may be closely related to drug resistance, but few studies are involved in osteosarcoma. Establishment of animal models and gene expression of VEGF and NF-κB1 may reveal the association between genes and drug resistance in osteosarcoma.
OBJECTIVE: To observe gene expression differences of VEGF and NF-κB1 in a nude mouse model of MG63 and MG63/ADR osteosarcoma.
METHODS: An adriamycin-resistant human osteosarcoma cell subline (MG63/ADR) was established in vitro using gradually increased concentration of adriamycin (ADM) in culture. BALB/C nude mice were divided into MG63 and MG63/ADR groups with 10 mice in each group. The animal model of osteosarcoma xenografts was constructed by subcutaneous inoculation of osteosarcoma MG63 and MG63/ADR cells respectively. Two group mice were killed at 6 weeks and gene expression differences of VEGF and NF-κB1 in the tumor formation of two groups were detected using immunohistochemistry and RT-PCR method.
RESULTS AND CONCLUSION: Both VEGF and NF-κB1 expression in MG63/ADR group was significantly higher compared with the MG63 group. The upregulation of VEGF and NF-κB1 may be an important reason of drug resistance in osteosarcoma, providing an experimental basis for exploring the molecular mechanism of drug resistance in osteosarcoma and finding more effective targets of osteosarcoma treatment.

中图分类号: